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Cloning of a cDNA Encoding Class II Specific DNA Binding Protein

  • Daniel K. Didier
  • Joel Schiffenbauer
  • Susan L. Woulfe
  • Benjamin D. Schwartz

Abstract

The class II molecules of the HLA complex are expressed primarily on immunocompetent cells including B-cells, activated T cells, and macrophages. A coordinate level of expression is seen between the class II α and β subunits. Recent studies have focused on the mechanisms underlying this coordinate level of expression, and have localized most regulatory control to the 5′ upstream region of the class II genes specifically to sequences that have been termed the Z (or W), X, and Y boxes (1–3). We designed our studies to isolate and characterize the regulatory proteins that interact with the X and Y boxes of the DRα gene. We found a number of nuclear proteins that bind to the class II box. Based on the cells from which the nuclear proteins were isolated, these proteins appear to be both negative and positive regulatory factors. We have directly cloned a cDNA corresponding to a gene that encodes a protein which appears relatively specific for the Y box, and the transcription of this gene appears inversely proportional to class II expression.

Keywords

Phorbol Myristate Acetate Coordinate Level Murine Sarcoma Virus Retarded Band Transcriptional Control Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Daniel K. Didier
    • 1
  • Joel Schiffenbauer
    • 1
  • Susan L. Woulfe
    • 1
  • Benjamin D. Schwartz
    • 1
  1. 1.Howard Hughes Medical Institute and Departments of Medicine and Microbiology and ImmunologyWashington University School of MedicineSt. LouisUSA

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