Cloning of a cDNA Encoding Class II Specific DNA Binding Protein
The class II molecules of the HLA complex are expressed primarily on immunocompetent cells including B-cells, activated T cells, and macrophages. A coordinate level of expression is seen between the class II α and β subunits. Recent studies have focused on the mechanisms underlying this coordinate level of expression, and have localized most regulatory control to the 5′ upstream region of the class II genes specifically to sequences that have been termed the Z (or W), X, and Y boxes (1–3). We designed our studies to isolate and characterize the regulatory proteins that interact with the X and Y boxes of the DRα gene. We found a number of nuclear proteins that bind to the class II box. Based on the cells from which the nuclear proteins were isolated, these proteins appear to be both negative and positive regulatory factors. We have directly cloned a cDNA corresponding to a gene that encodes a protein which appears relatively specific for the Y box, and the transcription of this gene appears inversely proportional to class II expression.
KeywordsPhorbol Myristate Acetate Coordinate Level Murine Sarcoma Virus Retarded Band Transcriptional Control Element
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Sullivan KE, Calman AF, Makamishi M, Tsang S, Wang Y, Peterlin BM. A model for the transcriptional regulation of MHC class II genes. Immunology Today 1987; 8: 289.CrossRefGoogle Scholar
Miwa K, Doyle C, Strominger JL. Sequence-specific interactions of nuclear factors with conserved sequences of human class II major histocompatibility complex genes. Proc Natl Acad Sci USA 1987; 84: 4939.PubMedCrossRefGoogle Scholar
Dorn A, Durand B, Marfing C, Le Meur M, Benoist C, Mathis D. Conserved major histocompatibility complex class II boxes-X and Y— are transcriptional control elements and specifically bind nuclear proteins. Proc Natl Acad Sci USA 1987; 84: 6249.PubMedCrossRefGoogle Scholar
Dorn A, Bollekens J, Staub A, Benoist C, Mathis D. A multiplicity of CCAAT box-binding proteins. Cell 1987; 50: 863.PubMedCrossRefGoogle Scholar
Dignam JD, Lebovitz RM, Roeder RG, Accurate transcriptional initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl Acids Res 1983; 11: 1475.PubMedCrossRefGoogle Scholar
Bauer B, Steinberg J, Laemmli VK, Weintraub H. The detection of DNA-binding proteins by protein blotting. Nucl Acids Res 1980; 8: 1.CrossRefGoogle Scholar
Strauss F, Varshaysky A. A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome. Cell 1984; 37: 889.PubMedCrossRefGoogle Scholar
© Springer Science+Business Media New York 1989