Abstract
HLA class II molecules were isolated from a large panel of Epstein Barr virus-transformed B-cell lines by immunoprecipitation with the monoclonal antibodies Tu22, IA3, and SPV-L3. The radiolabeled immunocomplexes were analyzed by two-D gel electrophoresis. Such assays demonstrated that SPV-L3 (not used in this Workshop; therefore, see reference 1) is truly locus-specific and isolates only one type of HLA-DQ molecules from HLA-DR-DQ homozygous B-cell lines that originate from consanguineous origin.
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Bontrop RE, Baas EJ, Otting N, Schreuder GMTh, Giphart MJ. Molecular diversity of HLA-DQ: DQ alpha chain isoelectric point differences and their relation to serologically defined HLA-DQ allospecificities. Immunogenetics 1987; 25: 305.
Lotteau V, Teyton L, Burroughs D, Charron D. A novel HLA class II molecule (DR -DQ) created by mismatched isotype pairing. Nature 1987; 329: 339.
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© 1989 Springer Science+Business Media New York
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Bontrop, R.E., Otting, N., Giphart, M.J. (1989). Comparative Analysis of the Two-Dimensional Gel Profiles of the Monoclonal Antibodies Tu22, IA3, and SPV-L3. In: Dupont, B. (eds) Immunobiology of HLA. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39946-0_110
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DOI: https://doi.org/10.1007/978-3-662-39946-0_110
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