Comparative Analysis of the Two-Dimensional Gel Profiles of the Monoclonal Antibodies Tu22, IA3, and SPV-L3
HLA class II molecules were isolated from a large panel of Epstein Barr virus-transformed B-cell lines by immunoprecipitation with the monoclonal antibodies Tu22, IA3, and SPV-L3. The radiolabeled immunocomplexes were analyzed by two-D gel electrophoresis. Such assays demonstrated that SPV-L3 (not used in this Workshop; therefore, see reference 1) is truly locus-specific and isolates only one type of HLA-DQ molecules from HLA-DR-DQ homozygous B-cell lines that originate from consanguineous origin.
KeywordsSialic Acid Large Panel Alpha Chain Positive Cell Line Human Immunol