Comparative Analysis of the Two-Dimensional Gel Profiles of the Monoclonal Antibodies Tu22, IA3, and SPV-L3

  • Ronald E. Bontrop
  • Nel Otting
  • Marius J. Giphart

Abstract

HLA class II molecules were isolated from a large panel of Epstein Barr virus-transformed B-cell lines by immunoprecipitation with the monoclonal antibodies Tu22, IA3, and SPV-L3. The radiolabeled immunocomplexes were analyzed by two-D gel electrophoresis. Such assays demonstrated that SPV-L3 (not used in this Workshop; therefore, see reference 1) is truly locus-specific and isolates only one type of HLA-DQ molecules from HLA-DR-DQ homozygous B-cell lines that originate from consanguineous origin.

Keywords

Electrophoresis Univer 

References

  1. 1.
    Bontrop RE, Baas EJ, Otting N, Schreuder GMTh, Giphart MJ. Molecular diversity of HLA-DQ: DQ alpha chain isoelectric point differences and their relation to serologically defined HLA-DQ allospecificities. Immunogenetics 1987; 25: 305.PubMedCrossRefGoogle Scholar
  2. 2.
    Lotteau V, Teyton L, Burroughs D, Charron D. A novel HLA class II molecule (DR -DQ) created by mismatched isotype pairing. Nature 1987; 329: 339.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Ronald E. Bontrop
    • 1
  • Nel Otting
    • 1
  • Marius J. Giphart
    • 1
  1. 1.Department of Immunohematology and Blood BankUniversity Hospital LeidenLeidenThe Netherlands

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