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Comparative Analysis of the Two-Dimensional Gel Profiles of the Monoclonal Antibodies Tu22, IA3, and SPV-L3

  • Ronald E. Bontrop
  • Nel Otting
  • Marius J. Giphart

Abstract

HLA class II molecules were isolated from a large panel of Epstein Barr virus-transformed B-cell lines by immunoprecipitation with the monoclonal antibodies Tu22, IA3, and SPV-L3. The radiolabeled immunocomplexes were analyzed by two-D gel electrophoresis. Such assays demonstrated that SPV-L3 (not used in this Workshop; therefore, see reference 1) is truly locus-specific and isolates only one type of HLA-DQ molecules from HLA-DR-DQ homozygous B-cell lines that originate from consanguineous origin.

Keywords

Sialic Acid Large Panel Alpha Chain Positive Cell Line Human Immunol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bontrop RE, Baas EJ, Otting N, Schreuder GMTh, Giphart MJ. Molecular diversity of HLA-DQ: DQ alpha chain isoelectric point differences and their relation to serologically defined HLA-DQ allospecificities. Immunogenetics 1987; 25: 305.PubMedCrossRefGoogle Scholar
  2. 2.
    Lotteau V, Teyton L, Burroughs D, Charron D. A novel HLA class II molecule (DR -DQ) created by mismatched isotype pairing. Nature 1987; 329: 339.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Ronald E. Bontrop
    • 1
  • Nel Otting
    • 1
  • Marius J. Giphart
    • 1
  1. 1.Department of Immunohematology and Blood BankUniversity Hospital LeidenLeidenThe Netherlands

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