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Subunit and Spatial Relationships of HLA-DQ Polymorphisms That Subdivide the DQw3 Specificity

  • S. F. Radka
  • K. Nelson
  • J. V. Johnston

Abstract

Polymorphism of the HLA-DQ subregion is more extensive than classical DQ serologic specificities presently define. Recently, a DQ polymorphism termed TA10 DQw7 was defined (1) which has 100% correlation with a restriction length polymorphism of the DQ β gene (2). The TA10 epitope was found to exist on a subset of β chains carrying the DQw3 epitope, which have an electrophoretic mobility distinct from that of TA10 DQw3+ β chains. To determine more precisely the spatial relationships between the TA10 and DQw3 epitopes, we have analyzed several monoclonal antibodies detecting these specificities by Western blotting and competitive inhibition of antibody binding.

Keywords

Restriction Fragment Length Polymorph Restriction Length Polymorphism Restriction Fragment Length Polymorph Analysis Restriction Fragment Length Polymorph Pattern Homozygous Typing Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Schreuder GMT, Doxiadis T, Parleviet J, Grosse-Wilde H. DR DQ LB and TA10 specificities of Ninth Workshop homozygous typing cells. In: Albert ED, Baur MP, Mayr WR (Eds.): Histocompatibility Testing. Berlin, Springer-Verlag, 1984, p 243.Google Scholar
  2. 2.
    Kim SJ, Holbeck SL, Nisperos B, Hansen JA, Maeda H, Nepom GT. Identification of a polymorphic variant associated with HLA-DQw3 and characterized by specific restriction sites within the DQ ß gene. Proc Natl Acad Sci USA 1985; 82: 8139.PubMedCrossRefGoogle Scholar
  3. 3.
    Radka SF, Scott RG, Stewart SA. Molecular complexity of HLA-DQw3: The TA10 determinant is located on a subset of DQw3 ß chains. Hum Immunol 1987; 18: 287PubMedCrossRefGoogle Scholar
  4. 4.
    Cohen BB, Moxley M, Crichton D, Deane DL, Steele CM. A mild procedure for separating polypeptide chains prior to immunoprecipitation and Western blotting analysis. J Immunol Meth 1984; 75: 99.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • S. F. Radka
    • 1
  • K. Nelson
    • 1
  • J. V. Johnston
    • 1
  1. 1.Genetic Systems CorpSeattleUSA

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