Subunit and Spatial Relationships of HLA-DQ Polymorphisms That Subdivide the DQw3 Specificity

  • S. F. Radka
  • K. Nelson
  • J. V. Johnston

Abstract

Polymorphism of the HLA-DQ subregion is more extensive than classical DQ serologic specificities presently define. Recently, a DQ polymorphism termed TA10 DQw7 was defined (1) which has 100% correlation with a restriction length polymorphism of the DQ β gene (2). The TA10 epitope was found to exist on a subset of β chains carrying the DQw3 epitope, which have an electrophoretic mobility distinct from that of TA10 DQw3+ β chains. To determine more precisely the spatial relationships between the TA10 and DQw3 epitopes, we have analyzed several monoclonal antibodies detecting these specificities by Western blotting and competitive inhibition of antibody binding.

Keywords

Electrophoresis Polypeptide FITC Hines Lori 

References

  1. 1.
    Schreuder GMT, Doxiadis T, Parleviet J, Grosse-Wilde H. DR DQ LB and TA10 specificities of Ninth Workshop homozygous typing cells. In: Albert ED, Baur MP, Mayr WR (Eds.): Histocompatibility Testing. Berlin, Springer-Verlag, 1984, p 243.Google Scholar
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    Radka SF, Scott RG, Stewart SA. Molecular complexity of HLA-DQw3: The TA10 determinant is located on a subset of DQw3 ß chains. Hum Immunol 1987; 18: 287PubMedCrossRefGoogle Scholar
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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • S. F. Radka
    • 1
  • K. Nelson
    • 1
  • J. V. Johnston
    • 1
  1. 1.Genetic Systems CorpSeattleUSA

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