Skip to main content

Plasma Albumin Synthesis in Perfused Rat Liver

  • Chapter
Stoffwechsel der isoliert perfundierten Leber

Abstract

14C labelled amino acids have been used extensively to study protein synthesis in vivo and in tissues. To evaluate the rate of synthesis from the 14C incorporation, it is necessary to know the specific activity of the amino acid at the site of synthesis. This is often difficult to establish. It has been frequently assumed that the specific activity of the amino acid in blood or tissue fluids equals that in the cell, but this may not be always true.

Supported by Grant A M — 07633 from the U. S. Public Health Service.

Work performed during tenure as Established Investigator of the American Heart Association.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 44.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 59.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Swick, R. W.: Measurement of protein turnover in rat liver. J. biol. Chem. 231, 751–764 (1958).

    PubMed  CAS  Google Scholar 

  2. Reeve, E. B., J. R. Pearson, and D. C. Marrz: Plasma protein synthesis in the liver: method for measurement of albumin formation in vivo. Science 139, 914–916 (1963).

    Article  PubMed  CAS  Google Scholar 

  3. Mcfarlane, A. S.: Measurements of synthesis rates of liver produced plasma proteins. Biochem. J. 89, 277–290 (1963).

    PubMed  CAS  Google Scholar 

  4. Mcfarlane, A. S., L. IRoNs, A. Koj, and E. Regoeczi: The measurement of synthesis rates of albumin and fibrinogen in rabbits. Biochem. J. 95, 536–540 (1965).

    PubMed  CAS  Google Scholar 

  5. Katz, J., G. Bonorris, and A. L. Sellers: Albumin metabolism in amino- nucleoside nephrotic rats. J. Lab. Clin. Med. 62, 910–934 (1963).

    PubMed  CAS  Google Scholar 

  6. Miller, L. L., C. G. Bly, M. L. Watson, and W. F. Bale: The dominant role of the liver in plasma protein synthesis. A direct study of the isolated perfused rat liver with the aid of lycine-E-C14. J. exp. Med. 94, 431–453 (1951).

    Article  PubMed  CAS  Google Scholar 

  7. Manometric techniques (3rd ed.). Edited by W. W. Umbreit, R. H. Burris, and J. F. Stauffer. Minneapolis: Burgess 1957, p. 149.

    Google Scholar 

  8. Gordon, A. H., and L. H. Mutschler: Synthesis rates of certain plasma proteins by the perfused rat liver. In: Protides of the biological fluids. Proc. 12th Colloq., Bruges, 1964. Edited by H. Peeters. Amsterdam: Elsevier 1965, p. 475.

    Google Scholar 

  9. John, D. W., and L. L. Miller: Influence of actinomycin D and puromycin on net synthesis of plasma albumin and fibrinogen by the isolated perfused rat liver. J. biol. Chem. 241, 4817–4824 (1966).

    PubMed  CAS  Google Scholar 

  10. Marsh, J. B., and D. L. Drabkin: Experimental reconstruction of metabolic pattern of lipid nephrosis: key role of hepatic protein synthesis in hyperlipemia. Metabolism 9, 946–955 (1960).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1968 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Katz, J., Sellers, A.L., Bonorris, G. (1968). Plasma Albumin Synthesis in Perfused Rat Liver. In: Staib, W., Scholz, R. (eds) Stoffwechsel der isoliert perfundierten Leber. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39856-2_8

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-39856-2_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-38916-4

  • Online ISBN: 978-3-662-39856-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics