Abstract
My interest in the biochemistry of alcohol arose recently from observations made in experiments on the rate of gluconeogenesis in the perfused liver in the presence of lactate and glycerol. On simultaneous addition of glycerol and lactate the rates of gluceneogenesis were found to be higher than the sum of the rates found with the two substrates alone. Thus, lactate alone formed glucose at a rate (μumole/min/g) of 1.06, glycerol of 0.48 and both together of 1.90. As glycerol, in the form of α-glycerophosphate, can act as a hydrogen donor in the cytoplasm we thought that the enhanced rate of gluconeogenesis might be connected with the reducing capacity of glycerol.
The work reported in this contribution was carried out in collaboration with Mr. R. Hems and Dr. R. A. Freeland.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Black, W. J.: Canad. J. Biochem. 44, 1301 (1966).
Blanchaer, M. C.: Canad. J. Biochem. 43, 17 (1965).
Bortz, W. M., and F. Lynen: Biochem. Z. 337, 505, 339, 77 (1963).
Brown, T. M., and A. M. Harvey: J. Amer. med. Ass. 117, 12 (1941).
Field, J. B., H. E. Williams, and G. E. Mortimore: J. clin. Invest. 42, 497 (1963).
Forsander, O., N. Raiha, and H. Suomalainen: Z. Physiol. Chem. 312, 243 (1958).
Forsander, O., N. Raiha, M. P. Salaspuro, P. Mäenpää: Biochem. J. 94, 259 (1965).
Freinkel, N., R. A. Arky, D. L. Singer, A. K. Cohen, S. J. Bleicher, J. B. Anderson, C. K. Silbert, and A. E. Foster: Diabetes, 14, 350 (1965).
Howard, C. F. Jr., and J. M. Lowenstein: J. biol. Chem. 240, 4170 (1965).
Isselbacher, K. J., and N. Greenberger: New Engl. J. Med. 270, 351 and 402 (1964).
Isselbacher, K. J., and S. M. Krane: J. biol. Chem. 236, 2394 (1961).
Kalckar, H. M., and E. S. Maxwell: Biochem. Biophys. Acta 22, 588 (1956).
Krebs, H. A.: Proc. roy. Soc. Ser. B. 159, 545 (1964).
Lieber, C. S. In: Biochemical factors in alcoholism. Ed. by R. P. Maickel, Oxford: Pergamon Press (1967).
Lieber, C. S., D. P. Jones, M. S. Losowsky and C. S. Davidson: J. clin. Invest. 41, 1863 (1962).
Lochner, A., and L. L. Madison: Clin. Res. 11, 40 (1963).
Lochner, A., J. Wulff, and L. L. Madison: Metabolism, 16, 1 (1967).
Maxwell, E. S.: J. biol. Chem. 229, 139 (1957).
Mendeloff, A. I.: J. clin. Invest. 33, 1298 (1954).
Meyerhof, O.: Die chemischen Vorgänge im Muskel, Berlin: J. Springer (1930).
Nikkila, E. A., and K. Ojala: Life Sci. 2, 717 (1963)
Nikkila, E. A., and K. Ojala: Proc. Soc. exp. Biol. Med. 113, 814 (1963).
Reboucas, G., and K. J. Isselbacher: J. clin. Invest. 40, 1355 (1961).
Robinson, E. A., H. M. Kalckar, H. Troedsson, and K. Sanford: J. biol. Chem. 241, 2737 (1966).
Salaspuro, M. P.: Scand. J. Clin. Invest. 18, Suppl. 92, 145 (1966).
Salaspuro, M. P., and P. H. Mcenpää: Biochem. J. 100, 768 (1966).
Schmidt, E., and F. W. Schmidt: Kiln. Wschr. 38, 957 (1960).
Scrutton, M. C., and M. F. Utter: J. biol. Chem. 240, 1 (1965).
Seligson, D., S. S. Waldstein, B. Gige, W. H. Mekowey, and Sbovow: Clin. Res. 1, 86 (1953).
Smith, M. E., and H. W. Newman: J. biol. Chem. 234, 1544 (1959).
Tubbs, P. K., and P. B. Garland: Biochem. J. 93, 550 (1964).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1968 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Krebs, H.A. (1968). Effects of Ethanol on Gluconeogenesis in the Perfused Rat Liver. In: Staib, W., Scholz, R. (eds) Stoffwechsel der isoliert perfundierten Leber. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39856-2_19
Download citation
DOI: https://doi.org/10.1007/978-3-662-39856-2_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-38916-4
Online ISBN: 978-3-662-39856-2
eBook Packages: Springer Book Archive