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Stoffwechsel der isoliert perfundierten Leber pp 142–154Cite as

Studies on the Mechanism of Fatty Acid and Glucagon Stimulated Gluconeogenesis in the Perfused Rat Liver

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Summary

Acetate, at a concentration of 5 or 10 mM, stimulated gluconeogenesis from lactate (10 mM) to the extent of 40% in the perfused, 24-hr. fasted rat liver. The rate of acetoacetate production was increased three-fold when acetate was present alone at a concentration of 5 or 10 mM. This increased ketogenesis was completely suppressed by lactate.

No increase in liver acetyl-CoA was observed during acetate-, oleate- or glucagon-stimulated gluconeogenesis. These data indicate that acetyl-CoA activation of pyruvic carboxylase can play no major role in the increased gluconeogenesis or ketogenesis under fasting conditions. By using lactate as substrate, one can exclude a „sparing effect“ as a possible reason for the observed effects. Since an increase in medium β-hydroxybutyrate/acetoacetate ratio occurs during oleate- and glucagon-stimulated gluconeogenesis, an increase in NADH provision for the triose phosphate dehydrogenase reaction can be a possible explanation for the observed stimulation.

Supported by the Deutsche Forschungsgemeinschaft, Bad Godesberg, Germany.

NATO Postdoctoral Fellow 1966–1967.

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References

  1. Struck, E., J. Ashmore, and O. Wieland: In G. Weber (Ed.): Advances in enzyme regulation, 4, p. 219, New York: Pergamon Press, Inc., 1966.

    Google Scholar 

  2. Williamson, J. R., R. A. Kreisberg, and P. W. Felts: Proc. Nat. Acad. Sci. 56, 247 (1966).

    Article  CAS  Google Scholar 

  3. Teufel, H., L. A. Menahan, J. C. Shipp, S. Boning, and O. Wieland: Fed. Europ. Biochem. Soc. (submitted ), 1967.

    Google Scholar 

  4. Haynes, R. C., Jr.: In G. Weber (Ed.): Advances in enzyme regulation, 3, p. 111, New York: Pergamon Press, Inc., 1965.

    Google Scholar 

  5. Willms, B.: This book, p. 118.

    Google Scholar 

  6. Williamson, J. R.: Biochem. J. 101, 11c (1966).

    PubMed  CAS  Google Scholar 

  7. Williamson, J. R., P. H. Wright, W. J. Malaisse, and J. Ashmore: Biochem. Biophys. Res. Communs. 24, 765 (1966).

    Article  CAS  Google Scholar 

  8. Penhos, J. C., C. H. Wu, J. Daunas, M. Reitman, and R. Levine: Diabetes 15, 740 (1966).

    PubMed  CAS  Google Scholar 

  9. Utter, M. F., D. B. Keech, and M. C. Scrutton: In G. Weber (Ed.): Advances in enzyme regulation, 2, p. 49, New York: Pergamon Press, Inc., 1964.

    Google Scholar 

  10. Hems, R., B. D. Ross, M. N. Berry, and H. A. Krebs: Biochem. J. 101, 284 (1966).

    PubMed  CAS  Google Scholar 

  11. Wollenberger, A., O. Ristau, and G. Schoffa: Pflüg. Arch. ges. Physiol. 270, 399 (1960).

    CAS  Google Scholar 

  12. Lowry, O. H., J. V. Passonneau, F. X. Hasselberger, and D. W. SCHULZ: J. Biol. Chem. 239, 18 (1964).

    PubMed  CAS  Google Scholar 

  13. Bücher, Th.: Biochim. Biophys. Acta. 1, 292 (1947).

    Article  Google Scholar 

  14. Buckel, W., and H. Eggerer: Biochem. Z. 343, 29 (1963).

    Google Scholar 

  15. Garland, P. B., D. Shepherd, and D. W. Yates: Biochem. J. 97, 587 (1965).

    PubMed  CAS  Google Scholar 

  16. Hepp, D., E. Prüsse, H. Weiss, and O. WIELAND: Biochem. Z. 344, 87 (1966).

    CAS  Google Scholar 

  17. Steele, R. G. D., and J. H. TORAH.: Principles and procedures of statistics. New York: McGraw-Hill Book Co., Inc., 1960.

    Google Scholar 

  18. Cohen, P. P.: J. Biol. Chem. 119, 333 (1937).

    CAS  Google Scholar 

  19. Drahota, Z., P. Hahn, A. Kleinzellner, and A. Kostolanska: Biochem. J. 93, 61 (1964).

    PubMed  CAS  Google Scholar 

  20. Jowett, M., and J. H. Quastel: Biochem. J. 29, 2159 (1935).

    PubMed  CAS  Google Scholar 

  21. Loeb, A.: Biochem. Z. 47, 118 (1912).

    Google Scholar 

  22. Friedmann, E.: Biochem. Z. 55, 436 (1913).

    Google Scholar 

  23. Embden, G., and A. Loeb: Biochem. Z. 88, 246 (1913).

    Google Scholar 

  24. Wieland, O., L. Weiss, and I. Eger-Neufeldt: In G. Weber (Ed.): Advances in enzyme regulation, 2, p. 85, New York: Pergamon Press, Inc., 1964.

    Google Scholar 

  25. Krebs, H. A.: In G. Weber (Ed.): Advances in enzyme regulation, 4, p. 339, New York: Pergamon Press, Inc., 1966.

    Google Scholar 

  26. Lynen, F., M. Matsuhashi, S. Numa, and E. Schweizer: In: The control of lipid metabolism. Biochem. Soc. Symp. No. 24, p. 43, New York: Academic Press, 1963.

    Google Scholar 

  27. Scholz, R.: This book, p. 25.

    Google Scholar 

  28. Krebs, H. A., R. N. Speake, and R. Hems: Biochem. J. 94, 712 (1965).

    PubMed  CAS  Google Scholar 

  29. Ross, B. D., R. Hems, and H. A. Krebs: Biochem. J. 102, 942 (1967).

    PubMed  CAS  Google Scholar 

  30. Sokal, J. E.: Endocrinology 78, 538 (1966).

    Article  PubMed  CAS  Google Scholar 

  31. Williamson, J. R., B. Herczeg, H. Coles, and R. Danish: Biochem. Biophys. Res. Communs. 24, 437 (1966).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Klinisch-Chemisches Institut, Städt. Krankenhaus München-Schwabing, Deutschland

    Lawrence A. Menahan, Brian D. Ross & Otto Wieland

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  1. Lawrence A. Menahan
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  2. Brian D. Ross
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  3. Otto Wieland
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Editor information

Editors and Affiliations

  1. Institut für Physiologische Chemie, Universitä Düsseldorf, Deutschland

    W. Staib

  2. Physiologisch-Chemisches Institut, Universität München, Deutschland

    R. Scholz

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Menahan, L.A., Ross, B.D., Wieland, O. (1968). Studies on the Mechanism of Fatty Acid and Glucagon Stimulated Gluconeogenesis in the Perfused Rat Liver. In: Staib, W., Scholz, R. (eds) Stoffwechsel der isoliert perfundierten Leber. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39856-2_12

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  • DOI: https://doi.org/10.1007/978-3-662-39856-2_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-38916-4

  • Online ISBN: 978-3-662-39856-2

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