Influence of Niclosamide and Phenprocoumone on the Level of Energy Phosphates and Selected Substrates of Glycolysis in Rat Liver

Abstract

In preliminary investigations we determined the intravenous LD₅₀ of both decoupling substances (niclosamide 8.25 mg/kg, phenprocoumone 55 mg/kg). We established a distinct increasing during 10 min to 1 h. We gained the liver sample by the double hasket (two sledges) and stop freeze method of Faupel et al.(Arch.Biochem.Biophys. 148, 509, 1972). We cooled the samples between aluminium blocks extremly cooled by liquid nitrogen (−195° C). We determined the anoxia time of the liver. This time is necessary to calculate the level of our substrates back to the zero point. In the following alkaline and acid decomposition we pretreated the samples for the photometric tests. We measured following substrates in 3 hours: ATP, ADP, AMP, glucose 6 phosphate, lactate, pyruvate, citrate, and glycogen. The substrates of the adenylate system changed in a typical kind after the administration of decoupling substances. ATP was decreased in the first 30 min. ADP and AMP increased in the same time. During the tests we established an enhancement of glycolysis. The glycogen level changed in correspondence to glucose 6 phosphate. The citrate level increased by niclosamide administration distinctly.