Advertisement

Nephrology pp 630-640 | Cite as

Rapid Cell Volume Regulation by the Mouse Medullary Thick Ascending Limb of Henle

  • Steven C. Hebert
  • Adam M. Sun

Summary

In this paper, we summarize our current knowledge regarding cell volume regulation in mouse medullary thick ascending loop of Henle (MTAL) cells. It has become apparent that arginine vasopressin (ADH) plays a central role in this process (at least in certain species). During antidiuresis ADH increases the rate of NaCl absorption by the MTAL, thereby enhancing the single effect of countercurrent multiplication. In addition, ADH is required for MTAL cells to regulate their volume in the more hypertonic environment. ADH appears to activate normally quiescent basolateral Na+:H+ exchangers which mediate Na+ uptake into MTAL cells during volume regulatory increase (VRI). This action of ADH may be mediated via an increase in cytosolic calcium. The trade off for this effect of ADH appears to be that the MTAL cells are no longer able to regulate their cell volume completely following reductions in interstitial osmolality. This is a direct result of an inverse relationship between the rates of salt absorption and volume regulatory decrease (VRD). This may not present a problem for the MTAL in vivo for two reasons. First, when interstitial osmolality is increased, NaCl absorption is reduced (see [1–3]). Second, when interstitial osmolality decreases during the transition from an antidiuretic to a water diuretic state the circulating level of ADH falls, and consequently, the rapid VRD response would be restored.

Keywords

Basolateral Membrane Arginine Vasopressin Renal Epithelial Cell Volume Regulatory Decrease Cell Volume Regulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hebert SC, Andreoli TE (1984) Control of NaCI transport in the thick ascending limb. Am J Physiol 246: F745–F75PubMedGoogle Scholar
  2. 2.
    Hebert SC, Culpepper RM, Andreoli TE (1981) NaCI transport in mouse medullary thick ascending limbs. III Modulation of the ADH effect by peritubular osmolality. Am J Physiol 241: F443–F45PubMedGoogle Scholar
  3. 3.
    Molony DA, Andreoli TE (1988) Diluting power of thick limbs of Henle: I. Peritubular hypertonicity blocks basolateral Cl channels. Am J Physiol 255: F1128–F1137PubMedGoogle Scholar
  4. 4.
    Hebert SC (1986) Hypertonic cell volume regulation in mouse thick limbs. I. ADH dependency and nephron heterogeneity. Am J Physiol 250: C907–C919PubMedGoogle Scholar
  5. 5.
    Hebert SC, Culpepper RM, Andreoli TE (1981) NaCI transport in mouse medullary thick ascending limbs. I Functional nephron heterogeneity and ADH-stimulated NaCI cotransport. Am J Physiol 241: F412–F431PubMedGoogle Scholar
  6. 6.
    Hebert SC (1986) Hypertonic cell volume regulation in mouse thick limbs. II. Na-H and Cl-HCO3 exchange in basolateral membranes. Am J Physiol 250: C920–C931PubMedGoogle Scholar
  7. 7.
    Hebert SC, Sun A (1988) Hypotonie cell volume regulation in mouse medullary thick ascending limb: effects of ADH. Am J Physiol 255: F962–F969PubMedGoogle Scholar
  8. 8.
    Kirk KL, Schafer JA, DiBona DR (1987) Cell volume regulation in rabbit proximal straight tubule in vitro. Am J Physiol 252: F922–F932PubMedGoogle Scholar
  9. 9.
    Lohr J, Grantham JJ (1986) Isovolumetric regulation in anistonic media: A new approach to study compensated cell volume regulation in isolated proximal S2 segments. Kidney Int 29: 419Google Scholar
  10. 10.
    Hoffmann EK, Simonsen LO (1989) Membrane mechanisms in volume and pH regulation in vertebrate cells. Physiol Rev 69: 315–382PubMedGoogle Scholar
  11. 11.
    Sun A, Hebert SC (1989) Rapid hypertonic cell volume regulation in the perfused inner medullary collecting duct. Kidney Int 36: 831–842PubMedCrossRefGoogle Scholar
  12. 12.
    Hebert SC, Friedman PA, Andreoli TE (1984) Effects of antidiuretic hormone on cellular conductive pathways in mouse medullary thick ascending limbs of Henle: I. ADH increases transcellular conductive pathways. J Membr Biol 80: 201–219Google Scholar
  13. 13.
    Reeves WB, McDonald GA, Mehta P, Andreoli TE (1989) Activation of K. channels in renal medullary vesicles by cAMP-dependent protein kinase. J Membr Biol 109: 65–72PubMedCrossRefGoogle Scholar
  14. 14.
    Taniguchi J, Guggino WB (1989) Membrane stretch: a physiological stimulator of Ca“-activated K. channels in thick ascending limb. Am J Physiol 257–26: F347–F352Google Scholar
  15. 15.
    Chamberlin ME, Strange K (1989) Anisotonic cell volume regulation: a comparative view. Am J Physiol 257 (26): C159–C173PubMedGoogle Scholar
  16. 16.
    Hebert SC (1987) Volume regulation in renal epithelial cells. Semin Nephrol 7: 48–60PubMedGoogle Scholar
  17. 17.
    Eveloff JL, Warnock DG (1987) Activation of ion transport systems during cell volume regulation. Am J Physiol 252: F1–F10PubMedGoogle Scholar
  18. 18.
    Blumenfeld JD, Grossman EB, Sun AM, Hebert SC (1989) Sodium-coupled ion cotransport and volume regulatory increase response. Kidney Int 36: 434–440PubMedCrossRefGoogle Scholar
  19. 19.
    Kikeri D, Azar S, Sun A, Zeidel ML, Hebert SC (1990) Na’:H’ antiporter and Na’(HCO3-)„ symporter regulate intracellular pH in mouse medullary thick limbs. Am J Physiol 258: F445–F456PubMedGoogle Scholar
  20. 20.
    Kikeri D, Sun A, Zeidel ML, Hebert SC (1989) Cell membranes impermeable to NH 3. Nature 339: 478–480PubMedCrossRefGoogle Scholar
  21. 21.
    Hebert SC, Sun A, Kikeri D (1990) Interrelationships among cell volume, pH and salt transport in thick ascending limb (TAL) and inner medullary collecting duct (IMCD) cells from mammalian kidney: Effects of vasopressin (abstract). Renal Physiol Biochem 13: 168–169Google Scholar
  22. 22.
    Sun A, Hebert SC (1990) Antidiuretic hormone (ADH) and cytosolic Cap’ regulate the basolateral (BI) Na’:H’ antiporter involved in the rapid volume regulatory increase (VRI) response of mouse medullary thick limbs ( MTAL) (abstract ). Clin Res 38: 313AGoogle Scholar
  23. 23.
    Grinstein S, Rothstein A (1986) Mechanism of regulation of the Na’/H’ exchanger. J Membr Biol 90: 1–12PubMedCrossRefGoogle Scholar
  24. 24.
    Ussing HH (1960) Active and passive transport of the alkali metal ions. In: Ussing HH, Kruhoffer P, Thaysen JH, Thorn NA (eds) The alkali metal ions in biology. Springer, Berlin, pp 45–143CrossRefGoogle Scholar
  25. 25.
    Weinman EJ, Shenolikar S, Kahn AM (1987) cAMP-associated inhibition of Na’-H’ exchanger in rabbit brush-border membranes. Am J Physiol 252: F19–F25Google Scholar
  26. 26.
    Borgese F, Garcia-Romeu F, Motias R (1987) Control of cell volume and ion transport by ß-adrenergic catecholamines in erythrocytes of rainbow trout, Salmo gairdneri. J Physiol (Lond) 382: 123–144Google Scholar
  27. 27.
    Grinstein S, Cohen S (1987) Cytoplasmic [Ca“] and intracellular pH in lymphocytes. Role of membrane potential and volume-activated Na’/H’ exchange. J Gen Physiol 89: 185–213Google Scholar
  28. 28.
    Hoffmann EK, Simonsen LO (1989) Membrane mechanisms in volume and pH regulation in vertebrate cells. Physiol Rev 69: 315–382PubMedGoogle Scholar
  29. 29.
    Mason MJ, Smith JD, Garcia-Soto JD-J, Grinstein S (1989) Internal pH-sensitive site couples CI--HCO3- exchange to Na’:H’ antiport in lymphocytes. Am J Physiol 256–25: C428–C433Google Scholar
  30. 30.
    Dobyan DC, Magill LS, Friedman PA, Hebert SC, Bulger RE (1982) Carbonic anhydrase histochemistry in rabbit and mouse kidneys. Anat Rec 204: 185–197PubMedCrossRefGoogle Scholar

Copyright information

© Springer Japan 1991

Authors and Affiliations

  • Steven C. Hebert
  • Adam M. Sun
    • 1
  1. 1.Laboratory of Molecular Physiology and Biophysics, Renal Division, Department of MedicineBrigham and Women’s HospitalBostonUSA

Personalised recommendations