Summary
The strategies that have been successfully employed to identify and characterize the brush border Na+/glucose cotransporter are summarized. These include the use of photoaffinity and covalent reagents, expression cloning, polyclonal antibodies to defined domains of the predicted sequence, and cDNA probes. The successful application of these strategies to renal membrane proteins will significantly advance the study of transporters for organic molecules.
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References
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© 1991 Springer Japan
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Wright, E.M. (1991). Strategies for Evaluating the Molecular Structure of Membrane Transport Systems. In: Hatano, M. (eds) Nephrology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-35158-1_141
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DOI: https://doi.org/10.1007/978-3-662-35158-1_141
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-70074-6
Online ISBN: 978-3-662-35158-1
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