Nephrology pp 1376-1379 | Cite as

Strategies for Evaluating the Molecular Structure of Membrane Transport Systems

  • Ernest M. Wright


The strategies that have been successfully employed to identify and characterize the brush border Na+/glucose cotransporter are summarized. These include the use of photoaffinity and covalent reagents, expression cloning, polyclonal antibodies to defined domains of the predicted sequence, and cDNA probes. The successful application of these strategies to renal membrane proteins will significantly advance the study of transporters for organic molecules.


Brush Border Expression Cloning Gaba Transporter Intestinal Brush Border Intestinal Brush Border Membrane 
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Copyright information

© Springer Japan 1991

Authors and Affiliations

  • Ernest M. Wright
    • 1
  1. 1.Department of PhysiologyUCLA School of MedicineLos AngelesUSA

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