Abstract
Any in vitro analysis of differentiation should take into account three important factors. One is the artifactual nature of the in vitro conditions. The very nature of the culture methodology creates an artifact. This is not meant to imply a deprecation of the study of a created artifact, for in many instances much useful information has been obtained from such studies. The investigator must be aware as to how much transference may be given to in vivo phenomena from in vitro studies. Another factor to take cognizance of is that negative aspects of differentiation in vitro (i. e. “dedifferen-tiation”, or the failure of differentiation) may not be a manifestation of a basic mechanism of differentiation. Observations of this sort may usually be ascribed to the fault of the experimenter and the conditions given the tissue in its foreign environment. A third point to be considered is that the phenotypic expression of differentiation may not be obvious to the observer and may require refined techniques of assay. A cell or tissue that appears “undifferentiated” may actually possess a differentiated metabolic pattern.
Supported by Grant HD-00380 from the National Institute of Child Health and Human Development, National Institutes of Health, and National Science Foundation Grant GB-3674 and GB-6748.
Career Development Awardee of the National Institute of Child Health and Human Development.
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References
Cahn, R. D., and M. B. Cahn: Heritability of cellular differentiation: Clonal growth and expression of differentiation in retinal pigment cells in vitro. Proc. nat. Acad. Sci. (Wash.) 55, 106–114 (1966).
Coon, H. G.: Clonal stability and phenotypic expression of chick cartilage cells in vitro. Proc. nat. Acad. Sci. (Wash.) 55, 66–73 (1966).
Cooper, G. W.: Induction of somite chondrogenesis by cartilage and notochord: A correlation between inductive activity and specific stages of cytodifferentiation. Develop. Biol. 12, 185–212 (1965).
Ellison, M., and E. J. Ambrose: Manuscript (1967).
Fabro, S. P., and L. M. Rinaldini: Loss of ascorbic acid synthesis in embryonic development. Develop. Biol. 11, 468–488 (1965).
Flower, M., and C. Grobstein: Interconvertibility of induced morphogenetic responses of mouse embryonic somites to notochord and ventral spinal cord. Develop. Biol. 15, 193–205 (1967).
Franco-Browder, S., J. de Rydt, and A. Dorfman: The identification of a sulfated mucopolysaccharide in chick embryos. Stages 11–23. Proc. nat. Acad. Sci. (Wash.) 49, 643–647 (1963).
Glick, M. C., J. W. Lash, and J. W. Madden: Enzymic activities associated with the induction of chondrogenesis in vitro. Biochim. biophys. Acta (Amst.) 83, 84–92 (1964).
Grobstein, C., and G. Parker: In vitro induction of cartilage in mouse somite mesoderm by embryonic spinal cord. Proc. Soc. exp. Biol. (N. Y.) 85, 477–481 (1954).
Holtzer, H.: An experimental analysis of the development of the spinal column. I. Response of pre-cartilage cells to size variations of the spinal cord. J. exp. Zool. 121, 121–148 (1952).
Holtzer, H.: Control of chondrogenesis in the embryo. Biophys. J. Suppl. 4, 239–250 (1964).
Johnston, P. M., and C. L. Comar: Autoradiographic studies of the utilization of S35-sulfate by the chick embryo. J. biophys. biochem. Cytol. 3, 231–238 (1957).
Lash, J. W.: Tissue interaction and specific metabolic responses: Chondrogenic induction and differentiation. In: Cytodifferentiation and macromolecular synthesis. Ed. M. Locke, p. 235–260. New York: Academic Press 1963.
Lash, J. W.: Normal embryology and teratogenesis. Amer. J. Obstet. Gynec. 90, 1193–1207 (1964).
Lash, J. W.: Differential behavior of anterior and posterior embryonic chick somites in vitro. J. exp. Zool. 165, 47–56 (1967).
Lash, J. W.: Somitic mesenchyme and its response to cartilage induction. In: Epithelial-mesenchymal interactions. Ed. R. Fleischmajer and R. Billingham, in press. Baltimore: Williams & Wilkins 1968.
Lash, J. W., S. Holtzer, and H. Holtzer: An experimental analysis of the development of the spinal column. VI. Aspects of cartilage induction. Exp. Cell Res. 13, 292–303 (1957).
Lash, J. W., F. A. Hommes, and F. Zilliken: Induction of cell differentiation. The in vitro induction of vertebral cartilage with a low-molecular weight tissue component. Biochim. biophys. Acta (Amst.) 56, 313–319 (1962).
Lash, J. W., M. C. Glick, and J. W. Madden: Cartilage induction in vitro and sulfate-activating enzymes. Nat. Canc. Inst. Monogr. 13, 39–49 (1964).
Lipmann, F.: Biological sulfate activation and transfer. Science 128, 575–580 (1958).
Marzullo, G., and J. W. Lash: Acquisition of the chondrocytic phenotype. In: Exp. Biol. Med. Vol. 1, pp. 213–218. Basel-New York: S. Karger 1967.
Medoff, J.: Enzymatic events during cartilage differentiation in chick embryonic limb bud. Develop. Biol. 16, 118–143 (1967).
Przybylski, R., and J. M. Blumberg: Ultrastructural aspects of myogenesis in the chick. Lab. Invest. 15, 836–863 (1966).
Rutter, W. J., W. D. Ball, W. S. Brandshaw, W. R. Clark, and T. G. Sanders: Levels of regulation in cytodifferentiation. In: Exp. Biol. Med. Vol. 1, pp. 110–124. Basel-New York: S. Karger 1967.
Searls, R. L.: Isolation of mucopolysaccharide from the pre-cartilaginous embryonic chick limb bud. Proc. exp. Biol. (N. Y.) 118, 1172–1176 (1965).
Strudel, G.: Influence Morphogene du Tube Nerveux et de la chorde sur la Differenciation de la Colonne Vertebrale. C. R. Soc. Biol. (Paris) 147, 132–133 (1953).
Strudel, G.: Induction de Cartilage in vitro par L’extrait du Tube Nerveux et de la Chorde de L’embryon de Poulet. Develop. Biol. 4, 67–86 (1962).
Strudel, G.: Some aspects of organogenesis of the chick spinal column. In: Exp. Biol. Med., Vol. 1, pp. 183–198, Basel-New York: S. Karger 1967.
Watterson, R. L.: Neural tube extirpation in Fundulus heteroclitus and resultant neural arch defects. Biol. Bull. 103, 310 (1952).
Wilt, F.: The ontogeny of chick embryo hemoglobin. Proc. nat. Acad. Sci. (Wash.) 48, 1582–1590 (1962).
Zilliken, F.: Notochord induced cartilage formation in chick somites. Intact tissues versus extracts. In: Exp. Biol. Med., Vol. 1, pp. 194–212. Basel-New York: S. Karger 1967.
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Lash, J.W. (1968). Phenotypic Expression and Differentiation: in vitro Chondrogenesis. In: Ursprung, H. (eds) The Stability of the Differentiated State. Results and Problems in Cell Differentiation, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-35089-8_2
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