Skip to main content
SpringerLink
Log in

Theory

  • Chapter
Gel Chromatography

Abstract

The experiment of Chapter 1 (page 6, cf. also Fig. 3) and a large number of experimental data which will be discussed in the following chapters demonstrate that solutes leave the bed of a granulated gel in the order of decreasing molecular weight as long as they have been applied jointly as a zone and have been washed through the column by the eluent. What happens in the gel bed is more easily understood if one assumes the following for diffusion equilibrium: The solvent in the swollen gel particles is not equally well accessible for molecules of different size (cf. Fig. 1). Only the solvent between the gel particles is flowing, and only its solutes will be advanced more rapidly than the smaller molecules which are in diffusion equilibrium with the resting gel phase (cf. Fig. 2).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Flodin, P.: J. Chromatog. 5, 103 (1961).

    Article  CAS  Google Scholar 

  2. Laurent, T. C., and J. Killander: ibid. 14, 317 (1964).

    Google Scholar 

  3. Granath, K. A.: J. colloid. Sci. 13, 308 (1958).

    Article  CAS  Google Scholar 

  4. Determann, H., und B. Gelotte in Rauen (Hrsg.): Biochemisches Taschenbuch, Band II, 906. Berlin, Göttingen, Heidelberg 1964.

    Google Scholar 

  5. Wheaton, R. M., and W. C. Baumann: Ann. N.Y. Acad. Sci. 57, 159 (1953).

    Google Scholar 

  6. Granath, K. A., and P. Flodin: Makromol. Chem. 48, 160 (1961).

    Google Scholar 

  7. Whitaker, J. R.: Anal. Chem. 35, 1950 (1963).

    Article  CAS  Google Scholar 

  8. Wicke, E.: Ber. Bunsenges. Physik. Chem. 69, 761 (1965).

    CAS  Google Scholar 

  9. Glueckauf, E., in: Ion exchange and its applications, Soc. Chem. Ind., 34. London 1965.

    Google Scholar 

  10. Purnell, G. G., in: Gas Chromatography, 108. New York: 1962.

    Google Scholar 

  11. Porath, J., and P. Flodin: Protides of the biol. Fluids 10, 297 (1963).

    Google Scholar 

  12. Smith, W. B., and A. KoLLMANSBERGER: J. phys. Chem. 69, 4157 (1965).

    Google Scholar 

  13. Flodin, P.: Dextran Gels and their Applications in Gel Filtration, Dissertation, Uppsala, 1962.

    Google Scholar 

  14. Laurent, T. C., and E. P. Laurent: J. Chromatog. 16, 89 (1964).

    Article  CAS  Google Scholar 

  15. Winzor, D. J., and L.W. Nichol: Biochim. biophys. Acta 104, 1 (1965).

    Google Scholar 

  16. Leach, A. A., and P. C. O’Shea: J. Chromatog. 17, 245 (1965).

    Google Scholar 

  17. Selby, K., and C. C. Maitland: Biochem. J. 94, 578 (1965).

    Google Scholar 

  18. Moore, J. C., and J. G. Hendrickson: J. polymer. Sci., Part C, 8, 233 (1965).

    Article  Google Scholar 

  19. Altgelt, K. H., and J. C. Moore: in Cantow (Ed.): Polymer Fractionation, p. 123, New York, 1967.

    Google Scholar 

  20. Porath, J.: Pure appl. Chem. 6, 233 (1963).

    Google Scholar 

  21. Zimm, B. FL, and W. H. Stockmayer: J. chem. Physics 17, 1301 (1949).

    Article  CAS  Google Scholar 

  22. Squire, P. G.: Arch. Biochem. Biophys. 107, 471 (1964).

    Article  CAS  Google Scholar 

  23. Laurent, T. C., and A. Pietruszkiewicz: Biochim. biophys. Acta 49, 258 (1961).

    CAS  Google Scholar 

  24. Laurent, T. C., and A. Pietruszkiewicz: Biochem. J. 89, 253 (1963).

    CAS  Google Scholar 

  25. Laurent, T. C., and A. Pietruszkiewicz: Acta chem. scand. 17, 2664 (1963).

    Google Scholar 

  26. Laurent, T. C., and A. Pietruszkiewicz: Biochem. J. 93, 106 (1964).

    CAS  Google Scholar 

  27. Laurent, T. C., and A. Pietruszkiewicz, K. Hellsing und B. Gelotte: Acta chem. scand. 18, 274 (1964).

    Article  CAS  Google Scholar 

  28. Ogstox, A. G.: Trans. Faraday Soc. 54, 1754 (1958).

    Article  Google Scholar 

  29. Hellsing, K.: Acta chem. scand. 19, 1791 (1965).

    Article  CAS  Google Scholar 

  30. Pedersen, K. O.: Arch. Biochem. Biophys., Suppl. 1, 157 (1962).

    CAS  Google Scholar 

  31. Ackers, G. K., and R. L. Steere: Biochim. biophys. Acta 59, 137 (1962).

    CAS  Google Scholar 

  32. Renkin, E. M.: J. gen. Physiol. 38, 225 (1955).

    Google Scholar 

  33. Ackers, G. K.: Biochemistry 3, 723 (1964).

    Article  CAS  Google Scholar 

  34. Siegel, L. M., and K. J. Monty: Biochim. biophys. Acta 112, 346 (1966).

    CAS  Google Scholar 

  35. Anderson, D. M.W., and J. F. Stoddart: Anal. chim. Acta 34, 401 (1966).

    Article  Google Scholar 

  36. Determann, H., und W. Michel: J. Chromatog. 25, 303 (1966).

    Article  CAS  Google Scholar 

  37. Takagi, T.: 3rd International Seminar on Gel Permeation Chromatography, Genf, Mai 1966.

    Google Scholar 

  38. BRÖNsted, J. N.: Z. physikal. Chem Bodenstein Festband 1931, 257.

    Google Scholar 

  39. Hohn, TH., und W. Pollmann: Z. Naturforsch. 18 b, 919 (1963).

    Google Scholar 

  40. Brewer, P. I.: Polymer 6, 603 (1965).

    Article  CAS  Google Scholar 

  41. Tiselius, A., J. PoRath, and P.-A. Aleertsson: Science 141, 13 (1963).

    CAS  Google Scholar 

  42. Porath, J.: Biochim. biophys. Acta 39, 193 (1960).

    CAS  Google Scholar 

  43. Gelotte, B.: J. Chromatog. 3, 330 (1960).

    Article  CAS  Google Scholar 

  44. Si Jung Yeh, and H. L. Frisch: J. polymer Sci. 27, 149 (1958).

    Google Scholar 

  45. Si Jung Yeh, and H. L. Frisch: J. polymer Sci. 27, 149 (1958).

    Google Scholar 

  46. Vaughan, M. F.: Nature 188, 55 (1960).

    Article  CAS  Google Scholar 

  47. Coatis-Jones, B.: Nature 191, 272 (1961).

    Article  Google Scholar 

  48. Marsden, N. V. B.: Ann. N.Y. Acad. Sci. 125, 428 (1965).

    Article  CAS  Google Scholar 

  49. Determann, H., und I. Walter: In preparation.

    Google Scholar 

  50. Craig, L. C., and A. Ansevix: Biochemistry 2, 1268 (1963).

    Article  CAS  Google Scholar 

  51. Sun, K., and A. H. Sehon: Can. J. Chem. 43, 969 (1965).

    Article  CAS  Google Scholar 

  52. Gelotte, B., and J. Porath: in Heftmann ( Ed.) Chromatography, New York 1966.

    Google Scholar 

  53. Somers, T. C.: Nature 209, 368 (1966).

    Article  CAS  Google Scholar 

  54. Bagdasarian, M., N. A. Matheson, R. L. M. Synge and M. A. YouNGsox: Biochem. J. 91, 91 (1964).

    CAS  Google Scholar 

  55. Carnegie, P. R.: Nature 206, 1128 (1965).

    Article  CAS  Google Scholar 

  56. Schwartz, A. N., A.W. G. Yee, and B. A. Zabin: J. Chromatog. 20, 154 (1965).

    Article  CAS  Google Scholar 

  57. Hayes, F. N., E. Hansbury, and V. E. Mitchell: J. Chromatog. 16, 410 (1964).

    Article  CAS  Google Scholar 

  58. Spitzt, H., H. Skrube und K. MÜLler: Microchim. Acta 1961, 296.

    Google Scholar 

  59. Posner, A. M.: Nature 198, 1161 (1963).

    Article  CAS  Google Scholar 

  60. George, W. H. S.: Nature 195, 155 (1962).

    Article  CAS  Google Scholar 

  61. Giddings, J. C., and K. L. Mallik: Anal. Chem. 38, 997 (1966).

    Article  CAS  Google Scholar 

  62. Fawcett, J. S., and C. J. O. R. Morris: Separation Sci. 1, 9 (1966).

    Article  CAS  Google Scholar 

  63. Grassmann, W., und G. Deffner: Z. physiol. Chem. 293, 89 (1953).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Organische Chemie, Universität Frankfurt am Main, Frankfurt am Main, Deutschland

    Dr. phil. nat. Helmut Determann (Privatdozent)

Authors
  1. Dr. phil. nat. Helmut Determann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1968 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Determann, H. (1968). Theory. In: Gel Chromatography. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-28706-4_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-28706-4_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-27223-7

  • Online ISBN: 978-3-662-28706-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation