Abstract
Next to chemical composition, size and weight are the most significant properties of a molecule. The molecular weight is frequently the decisive parameter to distinguish two molecules. The difference in molecular size plays a role in all conventional physical separation methods. The higher homologues of a series show usually higher melting and boiling points as well as lower solubility than related compounds of similar structure. For all practical purposes these differences are overlapping with other properties such as polarity or electrical charge density which determine the behavior of a substance during crystallization, distillation, extraction and during most of the chromatographic methods of separation. Sorting by size is a very common ordering principle in the macroscopic world. At the molecular level it was applied relatively late and in only a few isolated cases. It is also true for separations which are based on differences in molecular weight that other factors than the difference in molecular size play a role.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Svedberg, T., and K. O. Pedersen: The Ultrazentrifuge, Oxford, 1940. - Schachmann, H. K.: Ultracentrifugation in Biochemistry, New York 1955.
Hermans, J. J., and H. A. Ende in B. KE (Ed.): Newer Methods of Polymer Characterisation, 525. New York 1964. - R. Trautmann in D.W. Newman (Ed.): Instrumental Methods of Experimental Biology, 211. New York 1964.
Barrer, R. M.: Ber. Bunsenges. Physik. Chem. 69, 787 (1965).
Endeavour 23, 122 (1964).
British Chem. Engng. 7, 267 (1959).
Scheuermann, E. A.: Chemiker-Ztg. 20, 767 (1961).
Graham, TH.: Phil. Trans. Roy. Soc., London, 151, 183 (1861).
Craig, L. C., and W. Konigsberg: J. phys. Chem. 65, 166 (1961).
Pierce, J. G., and C. A. Free: Biochim. biophys. Acta 48, 436 (1961).
Rosenfeld, M.: ibid. 75, 241 (1963).
Ackers, G. K., and R. L. Steere: ibid. 59, 137 (1962).
Russell, B, J LevrTT and A. Polson: ibid. 79, 622 (1964).
Craig, L. C., and A. O. Pulley: Biochemistry 1, 89 (1962).
Richardson, R.W.: and A. Ansevin: ibid. 2, 1268 (1963).
Richardson, R.W.:, E. J. Harfenist, and A. C. Paladini: ibid. 3, 764, (1964).
Richardson, R.W.: Adv. anal. Chem. Instr. 4, 35 (1965)
Synge, R. L. M., and M. A. Youngson: Biochem. J. 78, 31 P (1961).
Signer, R., H. Hanni, W. Koestler, W. Rottenburg und P. v. Tavel: Helv. chim. Acta 29, 1894 (1946).
Craig, L. C., and T. P. King: J. Amer. chem. Soc. 77, 6620 (1955); 78, 4171 (1956).
Wieland, TH., H. Determann und E. Albrecht: Liebigs Ann. Chem. 633, 185 (1960).
Mould, D. L., and R. L. M. Synge: Biochem. J. 58, 571 (1954).
Allison, A. C., and J. H. Humphrey: Nature, 183, 1590 (1959).
Smithies, O.: Biochem. J. 61, 629 (1955).
Arch. Biochem. Biophys. Suppl. 1, 125 (1962).
Tosis, M. P.: Anal. Biochem. 13, 121 (1965).
Ornstein, L.: Ann. N.Y. Acad. Sci.121, 321 (1964); B. J. Davis: ibid. 121, 404 (1964).
Stauff, J.: Kolloidchemie, 665 ff. Berlin, Göttingen, Heidelberg 1960.
Hermans, P. H.: Gels, in Kruyt: (Ed.) Colloid Science, 2, 483. Amsterdam 1949.
Stauff, J.: Kolloidchemie, 669 ff. Berlin, Göttingen, Heidelberg 1960.
Porath, J., and P. Flodin: Nature 183, 1657 (1959).
Fasold, H., G. Gundlach, and F. Turba in Heftmann (Ed.): Chromatography, 406. New York 1961.
Pedersen, K. O.: Arch. Biochem. Biophys., Suppl. 1, 157 (1962).
HjertÉN, S., and R. Mosbach: Anal. Biochem. 3, 109 (1962).
MooRE, J. C.: J. Polym. Sci. A 2, 835 (1964).
Determann, H.: Angew. Chem. 76, 635 (1964); Internat. Ed. 3, 608 (1964).
Samuelson, O., Ref. in W. Lautsch: Angew. Chem. 57, 149 (1944).
Rauen, H. M., und K. Felix: Z. Physiol. Chem. 283, 139 (1948).
Kunin, R., and R. J. Meyers: Discuss. Faraday Soc. 7, 114 (1949).
Richardson, R.W.: Nature 164, 916 (1949).
Richardson, R.W.: J. Chem. Soc. 1951, 910.
Thompson, A. R.: Nature 169, 495 (1952).
Partridge, S. M.: Nature 169, 496 (1952).
Deuel, H., J. Souls und L. Anyas-Weisz: Heiv. chim. Acta 33, 2171 (1950).
Mikes, J. A.: J. polym. Sci. 30, 615 (1958)
WhearoN, R. M., and W. C. Baumann: Ann. New York Acad. Sci. 57, 159 (1953).
Clark, R.T.: Analytic. Chem. 30, 1676 (1958).
Tiselius, A.: Naturwiss. 37, 25 (1950); Adv. Prot. Chem 3, 67 (1947).
Deuel, H., and H. Neukom: Adv. in Chemistry Series 11, 51 (1954).
Lindqvist, B., and T. Storgards: Nature 175, 511 (1955).
Lathe, G. H., and C. R. J. Ruthven: Biochem. J. 62, 665 (1956).
Po’sox, A.: Biochim. biophys. Acta 19, 53 (1956).
Porath, J.: Clin. chim. Acta 4, 776 (1959).
BJÖRK, W., and J. Porath: Acta them. Scand. 13, 1256 (1959).
Flodin, P.: Dissertation Uppsala 1962.
PoisoN, A.: Biochim biophys. Acta 50, 565 (1961).
Steere, R. L., and G. K. Ackers: Nature 196, 475, (1962); 194, 114 (1962).
HjertÉN, S.: Arch. Biochem. Biophys. 99, 466 (1962).
Vaughan, M. F.: Nature 188, 55 (1960).
Coatis-Jones, B.: Nature 191, 272 (1961).
Brewer, P. J.: Nature 188, 934 (1960); 190, 625 (1961).
Determann, H., G. LÜben und TH. Wieland: Makromol. Chem. 73, 168 (1964).
Kunin, R., E. Meitzner, and N. Bortnick: J. Amer. chem. Soc. 84 305 (1962). Kunin, R., E. F. Meitzner, J. A. Oline, S. A. Fisher, and V. Frisch: I EC Prod. Res. Develop. 1, 140 (1962). — B. P. 932 125 and 932126.
Millar, J. R., D. G. Smith, W. E. Marr, and T. R. E. Kressman, J. them. Soc. 1963, 219. — B. P. 849 122.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1968 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Determann, H. (1968). Introduction. In: Gel Chromatography. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-28706-4_1
Download citation
DOI: https://doi.org/10.1007/978-3-662-28706-4_1
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-27223-7
Online ISBN: 978-3-662-28706-4
eBook Packages: Springer Book Archive