Measurement of Bacterial Biomass in Sandy Sediments
Bacteria have important roles in sediment processes such as nutrient cycling, decomposition of organic matter and as a food source for many animals. In shallow marine environments where macrophytes are the main primary producers, bacteria are a dominant component of the food chain because much of the organic matter cannot be digested (Fenchel, 1977). Accurate estimates of bacterial biomass are needed in studies on their trophic role, but these are difficult to obtain in sandy sediments. Direct measurement of bacteria stained with acridine orange has been used after homogenising the sand to release attached organisms, but these are minimum estimates because an undetermined number of bacteria can remain attached (Dale, 1974; Meyer-Reil et al., 1978).
KeywordsAcridine Orange Bacterial Biomass Shallow Marine Environment MURAMIC Acid Alkali Hydrolysate
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