Abstract
The 2′,3′-cyclic phosphates VII–XXXI were prepared for further studies of the function of the base in the ribonuclease-catalysed hydrolysis of ribonucleotide diesters. As seen with purine (XXIV, XXV, XXVIII), pyridine (XXIX), and pyridazine (XXXI) derivatives there is no absolute specificity for pyrimidine bases. Minimal requirement for monomeric substrates appears to be a mesomeric ring system with a keto-group in α-position to the β-glycosidic bond. Specific binding to the enzyme by position 3, 4, 5, or 6 of the base can be excluded. Arguments that the keto-group is not involved in the binding process are derived from kinetic experiments. Alterations at the base do not affect K m but only k +2 .
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Gassen, H.G., Witzel, H. (1967). Zum Mechanismus der Ribonuclease-Reaktion. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_8
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