Mechanism of Ribulose-Diphosphate Carboxydismutase Reaction
Ribulose-1,5-P 2 labelled with tritium in the C-3 position was converted to 2 moles of phosphoglyceric acid by the carboxydismutase. Over 98 % of the radioactivity was found in the water, less than 0.1 % being in phosphoglyceric acid, indicating that the proton lost from the 3 position of the substrate does not contribute to the formation of product. The tritiated substrate reacts at only 20 % the rate of the normal carrier species. Neither substrate nor product show significant proton exchange with the medium due to the enzyme.
KeywordsGlutathione Aldehyde Pyruvate Hydrazine Phospho
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