Abstract
The syntheses of poly-l-arginyl and of poly-l-histidyl RNase are described. Poly-l-arginyl RNase was obtained by guanidisation of poly-l-ornithyl RNase, which, in turn, was prepared from native RNase by reacting it with δ,N-trifluoroacetyl-α,N-carboxy-l-ornithine anhydride, with subsequent removal of the trifluoroacetyl groups with piperidine. Derivatives enriched with up to 71 arginine residues were prepared and studied. Poly-l-histidyl RNase was prepared by the reaction of RNase with the unprotected N-carboxy-l-histidine anhydride hydrochloride. Derivatives enriched with up to 22 histidine residues per RNase molecule were obtained.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Becker, R. R., and Stahmann, M A, J Biol. Chem. 204 (1953) 745.
Becker, R. R., in Polyaminoacids, Polypeptides and Proteins (edited by M. A. Stahmann) University of Wisconsin Press, Madison 1962, paper 29.
Anfinsen, C. B., Sela, M., and Cooke, J. P., J. Biol. Chem. 237 (1962) 1825.
Wilchek, M., Frensdorff, A., and Sela, M., Arch. Biochem. Biophys. 113 (1966) 742.
Frensdorff, A., and Sela, M., European J. Biochem. 1 (1967) 267.
Becker, R. R., and Sawada, E., Federation Proc. 22 (1963) 419.
Wellner, D., Silman, H. I., and Sela, M., J. Biol. Chem. 238 (1963) 1324.
Epstein, C. J., and Goldberger, R. F. J. Biol. Chem. 239 (1964) 1087.
Weil, L., and Seibles, T. S., Arch. Biochem. Biophys. 54 (1955) 368.
Barnard, E. A., and Stein, W. D., J. Mol. Biol. 1 (1959) 339.
Gundlach, H. G., Stein, W. H., and Moore, S., J. Biol. Chem. 234 (1959) 1754.
Crestfield, A. M., Stein, W. H., and Moore, S., J. Biol. Chem. 238 (1963) 2413; 2421.
Heinrikson, R. L., Stein, W. H., Crestfield, A. M., and Moore, S., J. Biol. Chem. 240 (1965) 2921.
Deavin, A., Mathias, A. P., and Rabin, B. R., Nature, 211 (1966) 252.
Ariely, S., Wilchek, M., and Patchornik, A., Biopolymers, 4 (1966) 91.
Habeeb, A. F. S. A., Can. J. Biochem. Physiol. 38 (1960) 493.
Patchornik, A., Berger, A., and Katchalski, E., J. Am. Chem. Soc. 79 (1957) 6416.
Patchornik, A., and Shalitin, Y., Anal. Chem. 33 (1961) 1887.
Hirs, C. H. W., Moore, S., and Stein, W. H., J. Biol. Chem. 219 (1956) 623.
Yphantis, D. A., Ann. N. Y. Acad. Sci. 88 (1960) 586.
Harrington, W. F., and Schellman, J. A., Compt. Rend. Tray. Lab. Carlsberg (Ser. Chim.), 30 (1956) 21.
Cohn, E. J., and Edsall, J. T., “Proteins, Amino Acids and Peptides”. Reinhold Publ. Cor. N. Y., 1943.
Ellman, G. L., Arch. Biochem. Biophys. 82 (1959) 70.
Wilchek, M., Frensdorff, A., and Sela, M., Biochemistry, 6 (1967) 247.
Goldberger, R. F., and Anfinsen, C. B., Biochemistry, 1 (1962) 401.
Sela, M., Arnon, R., and Jacobson, I., Biopolymers, 1 (1963) 517.
Klee, W. A., and Richards, F. M., J. Biol. Chem. 229 (1957) 489.
Kuriyama, Y., J. Biochem. (Tokyo),59 (1966) 596.
Kalnitsky, G., Hummel, J.P., Resnick, H., Carter, J.R., Barnett, L. B., and Dierks, O., Ann. N. Y. Acad. Sci. 81 (1959) 541.
Trie, M., J. Biochem. (Tokyo), 57 (1965) 355.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1967 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Frensdorff, A., Wilchek, M., Sela, M. (1967). A Comparative Study of Basic, Acidic and Neutral Polypeptidyl RNases, Including Polyarginyl and Polyhistidyl Derivatives. In: Liébecq, C. (eds) European Journal of Biochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-25813-2_39
Download citation
DOI: https://doi.org/10.1007/978-3-662-25813-2_39
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-23717-5
Online ISBN: 978-3-662-25813-2
eBook Packages: Springer Book Archive