The Participation of GTP-AMP-P Transferase in Substrate Level Phosphate Transfer of Rat Liver Mitochondria

  • H. W. Heldt
  • K. Schwalbach

Abstract

From kinetic studies on the reaction sequence of substrate level phosphorylation in rat liver mitochondria, using anaerobic ketoglutarate dismutation in the presence of oligomycin and [32P]phosphate, phosphohistidine appears to be the first intermediate to be labelled, followed by GTP. [32P]ADP rather than [32P]ATP is shown to be the main product of the reaction. The phosphorylation of AMP requires ketoglutarate and is stimulated by 2,4-dinitrophenol.

Keywords

Sucrose EDTA Respiration Nucleoside Guanine 

Non-standard Abbreviations

TRA

Triethanolamine

NuDiKi

nucleoside diphosphate kinase

HK

hexokinase

G6PDH

glucose-6-phosphate dehydrogenase

AdK

adenylate kinase

P-His

phosphohistidine

DNP

2,4-dinitrophenol

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Heldt, H. W., in Regulation of metabolic processes in mitochondria (edited by J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater ), Elsevier, Amsterdam 1966, p. 51.Google Scholar
  2. 2.
    Heldt, H. W., Abstr. Z. Klin. Chem. 3 (1965) 203.Google Scholar
  3. 3. Heldt, H. W., and Schwalbach, K., Abstracts 3 FEBS Meeting (Warsaw),1966, M 42.Google Scholar
  4. 4.
    Chiga, M., Rogers, A. E., and Plaut, G. W. E., J. Biol. Chem. 236 (1961) 1800.PubMedGoogle Scholar
  5. 5.
    Heppel, L. A., Strominger, J. L., and Maxwell, E., Biochim. Biophys. Acta, 32 (1959) 422.PubMedCrossRefGoogle Scholar
  6. 6.
    Heldt, H. W., and Klingenberg, M., Biochem. Z. 343 (1965) 433.PubMedGoogle Scholar
  7. 7.
    Klingenberg, M., and Slenczka, W., Biochem. Z. 331 (1959) 486.PubMedGoogle Scholar
  8. 8.
    Kröger, A., and Klingenberg, M., Biochem. Z. 344 (1966) 317.PubMedGoogle Scholar
  9. 9.
    Delbrück, A., Zebe, E., and Bücher, Th., Biochem. Z. 331 (1959) 273.Google Scholar
  10. 10.
    Pfleiderer, G., Biochim. Biophys. Acta, 47 (1961) 389.PubMedCrossRefGoogle Scholar
  11. 11.
    Berg, P., and Joklik, W. K., J. Biol. Chem. 210 (1954) 657.PubMedGoogle Scholar
  12. 12.
    Oliver, I. T., Biochem. J. 61 (1955) 116.PubMedGoogle Scholar
  13. 13.
    Cha, S., and Parks, R. E., J. Biol. Chem. 239 (1964) 1961.PubMedGoogle Scholar
  14. 14.
    Kreil, G., and Boyer, P. D., Biochem. Biophys. Res. Commun. 16 (1964) 551.PubMedCrossRefGoogle Scholar
  15. 15.
    Mitchel, R. A., Butler, L. G., and Boyer, P. D., Biochem. Biophys. Res. Commun. 16 (1964) 545.CrossRefGoogle Scholar
  16. 16.
    Slater, E. C., and Kemp, A., Nature, 204 (1964) 1268.PubMedCrossRefGoogle Scholar
  17. 17.
    Heldt, H. W., Jacobs, H., and Klingenberg, M., Biochem. Biophys. Res. Commun. 17 (1964) 130.CrossRefGoogle Scholar
  18. 18.
    Klingenberg, M., and Pfaff, E., XXX, in Regulation of metabolic processes in mitochondria (edited by J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater), Elsevier, Amsterdam 1966, vol. 7, p. 180.Google Scholar
  19. 19.
    Ernster, L., XXX, in Round table conference on mitochondrial structure and compartmentation (edited by J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater), Editrice Adriatica, Bari 1966, In press.Google Scholar
  20. 20.
    Schwalbach, K., Unpublished results.Google Scholar
  21. 21.
    Heldt, H. W., Jacobs, IL, and Klingenberg, M., Biochem. Biophys. Res. Commun. 18 (1965) 174.PubMedCrossRefGoogle Scholar
  22. 22.
    Pfaff, E., Klingenberg, M., and Heldt, H. W., Biochim. Biophys. Acta, 104 (1965) 312.PubMedCrossRefGoogle Scholar
  23. 23.
    Bergh, S. G. Van den, in Round table conference on mitochondrial structure and compartmentation (edited by J. M. Tager, S. Papa, E. Quagliariello, and E. C. Slater), Editrice Adriatica, Bari 1966, In press.Google Scholar
  24. 24.
    Siekewitz, P., and Potter, V. R., J. Biol. Chem. 215 (1955) 237.Google Scholar
  25. 25.
    Biber, L.L., Lindberg, O., Duffy, J.J., and Boyer, P.D., Nature, 202 (1964) 1316.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1967

Authors and Affiliations

  • H. W. Heldt
    • 1
  • K. Schwalbach
    • 1
  1. 1.Physiologisch-chemisches InstitutPhilipps-UniversitätMarburg/LahnGermany

Personalised recommendations