Sheep Kidney Nuclease Hydrolysis of tRNA

  • K. Kasai
  • M. Grunberg-Manago


An endonuclease showing no apparent base specificity, nor any preference for the sugar moiety has been isolated from sheep kidney The products of the digestion are 5 -P-ended oligonucleo­tides, and not mononucleotides. Preparation of short oligo A and oligo U (di-, tri-, tetra-) with a good yield is described. The enzyme requires Mg ions, is inhibited at high ionic strength and destroyed by temperatures of 60° and above.


Incubation Mixture Paper Electrophoresis Acid Soluble Fraction Average Chain Length Phenol Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Holley, R. W., Apagar, J., Everett, G. A., Madison, J. T., Marquisee, M., Merril, S. H., Penswick, J. R., and Zamir, A., Science, 147 (1965) 1462.PubMedCrossRefGoogle Scholar
  2. 2.
    Zachau, H. G., Dütting, D., and Feldman, H., Angew. Chem. 78 (1966) 393.CrossRefGoogle Scholar
  3. 3.
    Madison, J. T., Everett, G. A., and Kung, H., Science, 153 (1966) 531.PubMedCrossRefGoogle Scholar
  4. 4.
    Singer, M. F., and Tolbert, G., Science, 145 (1964) 593. Spahr, P. F., J. Biol. Chem. 239 (1964) 3716.Google Scholar
  5. 5.
    Grunberg-Manago, M., J. Mol. Biol. 1 (1959) 240.CrossRefGoogle Scholar
  6. 6.
    Ando, T., Biochim. Biophys. Acta, 114 (1966) 158.PubMedCrossRefGoogle Scholar
  7. 7.
    Linn, S., and Lehmann, I. R., J. Biol. Chem. 240 (1965) 1287, 1294.Google Scholar
  8. 8.
    Curtis, P. J., Burdan, M. G., and Smellie, R. M. S., Biochem. J. 98 (1966) 813.PubMedGoogle Scholar
  9. 9.
    Curtis, P. J., and Smellie, R. M. S., Biochem. J. 98 (1966) 818.PubMedGoogle Scholar
  10. 10.
    Litt, M., and Ingram, V. M., Biochemistry, 3 (1964) 560.PubMedCrossRefGoogle Scholar
  11. 11.
    Armstrong, A., Hagopian, H., Ingram, V. M., and Wagner, E. K., Biochemistry, 5 (1966) 3027.PubMedCrossRefGoogle Scholar
  12. 12.
    Keselev, L. L., Frovola, L. Y., Borisova, O. F., and Kukhanova, M. K., Biokhimiya, 29 (1964) 116.Google Scholar
  13. 13.
    Wagner, E. K., and Ingram, V. M., Biochemistry, 5 (1966) 3019.PubMedCrossRefGoogle Scholar
  14. 14.
    Healey, J. W., Stollar, D., Simon, M. I., and Levine, L., Arch. Biochem. Biophys. 103 (1963) 461.CrossRefGoogle Scholar
  15. 15.
    Ingram, V. M., and Pierce, J. G., Biochemistry, 1 (1962) 580.CrossRefGoogle Scholar
  16. 16.
    Van Rapenbusch, R., and Deshepper, J. C., Compt. Rend. 262 (1966) 1365.Google Scholar
  17. 17.
    Felsenfeld, G., and Rich, A., Biochim. Biophys. Acta, 26 (1957) 457.PubMedCrossRefGoogle Scholar
  18. 18.
    Blake, R. D., and Fresco, J. R., J. Mol. Biol. 19 (1966) 145. Massoulie, J., Thèse de Doctorat, Univ. Paris, Juin 1966. Rich, A., Biochim. Biophys. Acta, 29 (1958) 502.CrossRefGoogle Scholar
  19. 19.
    Haselkorn, R., and Doty, P., J. Biol. Chem. 236 (1961) 2738.PubMedGoogle Scholar
  20. 20.
    Holcomb, D. N., Tinoco, I., jr., Biopolymer, 3 (1965) 121.CrossRefGoogle Scholar
  21. 21.
    Heppel, L. A., Ortiz, P. J., and Ochoa, S., Science, 123 (1956) 415.PubMedCrossRefGoogle Scholar
  22. 22.
    Fresco, J. R., and Alberts, B. M., Proc. Natl. Acad. Sci. U. S. 43 (1960) 311.CrossRefGoogle Scholar
  23. 23.
    Thang, M. N., Guschlbauer, W., Zachau, H. G., and Grunberg-Manago, M., J. Mol. Biol. In press. Monier, R., and Grunberg-Manago, M., Acides Ribonucl. et olyphosph. C.N.R.S., Paris 1962, p. 163. Singer, M. F., Luborsky, S., Morrison, R. A., and Canton, G. L., Biochim. Biophys. Acta, 38 (1960) 568.Google Scholar
  24. 24.
    Zamir, A., Holley, R. W., and Marquisee, M., J. Biol. Chem. 240 (1965) 1267.PubMedGoogle Scholar
  25. 25.
    Dunn, D. B., Smith, J. D., and Spahr, P. F., J. Mol. Biol. 2 (1960) 113.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1967

Authors and Affiliations

  • K. Kasai
    • 1
    • 2
  • M. Grunberg-Manago
    • 1
  1. 1.Institut de Biologie Physico-chimiqueParis
  2. 2.Institut de Biologie Physico Chimique75 Paris-5France

Personalised recommendations