Sheep Kidney Nuclease Hydrolysis of tRNA

  • K. Kasai
  • M. Grunberg-Manago

Abstract

An endonuclease showing no apparent base specificity, nor any preference for the sugar moiety has been isolated from sheep kidney The products of the digestion are 5 -P-ended oligonucleo­tides, and not mononucleotides. Preparation of short oligo A and oligo U (di-, tri-, tetra-) with a good yield is described. The enzyme requires Mg ions, is inhibited at high ionic strength and destroyed by temperatures of 60° and above.

Keywords

Cellulose Phenol Toluene Uranium Sedimentation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1967

Authors and Affiliations

  • K. Kasai
    • 1
    • 2
  • M. Grunberg-Manago
    • 1
  1. 1.Institut de Biologie Physico-chimiqueParis
  2. 2.Institut de Biologie Physico Chimique75 Paris-5France

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