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Properties of the c-Myc Protein

  • Chi V. Dang
  • Linda A. Lee
Part of the Medical Intelligence Unit book series (MIU.LANDES)

Abstract

Biology is full of surprises, and the regulation of c-myc readily illustrates this point. The regulation of c-myc expression is an intricate network of commands that controls transcriptional initiation, elongation as well as mRNA stability. In addition to this hierarchy of controls, production of the c-Myc polypeptides is also regulated. The predicted size of the c-myc encoded polypeptide initiated at the canonical AUG translational start site is 439 amino acids.1 The corresponding ATG is located at the 5' end of exon 2 (Fig. 4.1). Although the predicted molecular mass is 49.5 kDa, the observed size in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is about 62 kDa, which is largely due to the amino acid composition rather than to posttranslational modification.2–4 Hence this protein is termed p62Myc. From the same c-myc mRNAs, an alternative form of c-Myc polypeptide is translationally initiated at a CUG, 14 codons upstream from the canonical AUG (Fig. 6.1).5 This alternative form is termed p64Myc. These two forms of c-Myc appear as a doublet of polypeptides on SDS-PAGE.

Keywords

Casein Kinase Nuclear Matrix Murine Erythroleukemia Cell Nuclear Colocalization Internal Translational Initiation Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Chi V. Dang
    • 1
  • Linda A. Lee
    • 1
  1. 1.School of MedicineThe Johns Hopkins UniversityBaltimoreUSA

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