The Triphasic Sequence of Neo-Intima Formation in Cuffed Rabbit Carotid Arteries

  • Mark M. Kockx
Part of the Medical Intelligence Unit book series (MIU.LANDES)


Diffuse intimai thickening occurs commonly in arteries of man and increases progressively throughout life.1 Eccentric intimai thickenings (intimai cushions), associated with branches and orifices, have been observed in human arteries from the first week. Both the diffuse intimal thickening and the intimal cushions are considered as predilection sites for atherosclerosis.2–5 While the factors responsible for the intima development in man are largely unknown, numerous methods of injury have been applied to produce intimai lesions in animals. These methods can be divided into two broad categories: those that use intraluminal (e.g. balloon denudation) and those that use perivascular manipulation. Examples of the latter are external electrical stimulation,6 external compression,7 stripping the adventitia from arteries8 and positioning of a cuff around an artery.9,10 The perivascular cuff placement is used to avoid direct injury to the vessel wall, particularly to the endothelium. In certain experimental protocols rigid polyethylene tubes are used which still results in endothelial cell loss.9,11 We applied a perivascular method by placing a nonocclusive, biologically inert, soft and flexible silicone cuff around an artery as first described by Booth.10


Carotid Artery Proliferate Cell Nuclear Antigen Internal Elastic Lamina Medial Smooth Muscle Cell Rabbit Carotid Artery 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Mark M. Kockx
    • 1
  1. 1.Department of PathologyAlgemeen Ziekenhuis MiddelheimAntwerpBelgium

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