Pre-mRNA Splicing in Plants

  • Witold Filipowicz
  • Marek Gniadkowski
  • Ueli Klahre
  • Hong-Xiang Liu
Part of the Molecular Biology Intelligence Unit book series (MBIU)

Abstract

This chapter is focused on the splicing of nuclear pre-mRNAs in higher plants. Other aspects of plant mRNA processing such as polyadenylation and mRNA stability are discussed in recent reviews.1, 2 Nothing is known about the localization of pre-mRNA processing events in the plant nucleus or mRNA transport to the cytoplasm.

Keywords

Maize Mold Polypeptide Pyruvate Resi 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hunt AG. Messenger RNA 3′ end formation in plants. Annu. Rev. Plant Physiol. Plant Mol Biol 1994; 45:47–60.CrossRefGoogle Scholar
  2. 2.
    Sullivan ML, Green PJ. Post-transcriptional regulation of nuclear-encoded genes in higher plants: the roles of mRNA stability and translation. Plant Mol Biol 1993; 23:1091–1104.PubMedCrossRefGoogle Scholar
  3. 3.
    Larkin R, Guilfoyle T. The second largest subunit of RNA polymerase II from Arabidopsis thaliana. Nucl Acids Res 1993; 21:1038.PubMedCrossRefGoogle Scholar
  4. 4.
    Sheen J. Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes. Plant Cell 1991; 3:225–245.PubMedGoogle Scholar
  5. 5.
    Takei Y, Yamauchi D, Minamikawa T. Nucleotide sequence of the canavalin gene from Canavalia gladiata seeds. Nucl Acids Res 1989; 17:43–81.CrossRefGoogle Scholar
  6. 6.
    Goodall GJ, Filipowicz W. The minimum functional length of pre-mRNA introns in monocots and dicots. Plant Mol Biol 1990; 14:727–733.PubMedCrossRefGoogle Scholar
  7. 7.
    Goodall GJ, Kiss T, Filipowicz W. Nuclear RNA splicing and small nuclear RNAs and their genes in higher plants. Oxf Surv Plant Cell Mol Biol 1991; 7:255–296.Google Scholar
  8. 8.
    Grotewold E, Athma P, Peterson T. Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors. Proc Natl Acad Sci USA 1991; 88:4587–4591.PubMedCrossRefGoogle Scholar
  9. 9.
    McCullough AJ, Lou H, Schuler MA. Factors affecting authentic 5′ splice site selection in plant nuclei. Mol Cell Biol 1993; 13:1323–1331.PubMedGoogle Scholar
  10. 10.
    Goodall GJ, Filipowicz W. Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants. EMBO J 1991; 10:2635–2644.PubMedGoogle Scholar
  11. 11.
    Orozco BM, Robertson McClung C, Werneke JM, Ogren WL. Molecular basis of the ribulose-1, 5-bisphosphate carboxylase/ oxygenase activase mutation in Arabidopsis thaliana is a guanine-to-adenine transition at the 5′-splice junction of intron 3. Plant Physiol 1993; 102:227–232.PubMedCrossRefGoogle Scholar
  12. 12.
    Wiebauer K, Herrero J-J, Filipowicz W. Nuclear pre-mRNA processing in plants: distinct modes of 3′-splice site selection in plants and animals. Mol Cell Biol 1988; 8:2042–2051.PubMedGoogle Scholar
  13. 13.
    Goodall GJ, Filipowicz W. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing. Cell 1989; 58:473–483.PubMedCrossRefGoogle Scholar
  14. 14.
    Csank C, Taylor FM, Martindale DW. Nuclear pre-mRNA introns: analysis and comparison of intron sequences from Tetrahymena thermophila and other eukaryotes. Nucl Acids Res 1990; 18:5133–5141.PubMedCrossRefGoogle Scholar
  15. 15.
    Sinibaldi RM, Mettler IJ. Intron splicing and intron-mediated enhanced expression in monocots. Progr Nucl Acid Res Mol Biol 1992; 42:229–257.CrossRefGoogle Scholar
  16. 16.
    Green MR. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu Rev Cell Biol 1991; 7: 559–99.PubMedCrossRefGoogle Scholar
  17. 17.
    Moore MJ, Query CC, Sharp PA. Splicing of precursors to messenger RNAs by the spiceosome. In: Gesteland R, Atkins J, eds. The RNA World. Cold Spring Harborfg Lab Press 1993; 303–358.Google Scholar
  18. 18.
    Solymosy F, Pollák T. Uridylate-rich small nuclear RNAs (UsnRNAs), their genes and pseudogenes, and UsnRNPs in plants: structure and function. A comparative approach. Crit Rev Plant Sci 1993; 12: 275–369.Google Scholar
  19. 19.
    Hanley BA, Schuler MA. Developmental expression of plant snRNAs. Nucleic Acids Res 1991; 19: 6319–6325.PubMedCrossRefGoogle Scholar
  20. 20.
    Wise JA. Guides to the heart of the spliceosome. Science 1993; 262: 1978–1979.PubMedCrossRefGoogle Scholar
  21. 21.
    Palfi Z, Bach M, Solymosy F, Lührmann R. Purification of the major UsnRNPs from broad bean nuclear extracts and characterization of their protein constituents. Nucl Acids Res 1989; 17:1445–1458.PubMedCrossRefGoogle Scholar
  22. 22.
    Kulesza H, Simpson GG, Waugh R, Beggs JD, Brown JWS. Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8. FEBS lett 1993; 318:4–6.PubMedCrossRefGoogle Scholar
  23. 23.
    Simpson GG, Vaux P, Clark G, Waugh R, Beggs JD, Brown JWS. Evolutionary conservation of the spliceosomal protein, U2B″. Nucl Acids Res 1991; 19: 5213–5217.PubMedCrossRefGoogle Scholar
  24. 24.
    Reddy ASN, Czernik AJ, Gynheung A, Poovaiah BW. Cloning of the cDNA for U 1 small nuclear ribonucleoprotein particle 70K protein from Arabidopsis thaliana. Biochim Biophys Acta 1992; 1171:88–92.PubMedCrossRefGoogle Scholar
  25. 25.
    Dreyfuss G, Matunis MJ, Piñol-Roma S, Burd CG. hnRNP proteins and the biogenesis of mRNA. Annu Rev Biochem 1993; 62:289–321.PubMedCrossRefGoogle Scholar
  26. 26.
    McCullough AJ, Lou H, Schuler MA. In vivo analysis of plant pre-mRNA splicing using an autonomously replicating vector. Nucl Acids Res 1991; 19:3001–3009.PubMedCrossRefGoogle Scholar
  27. 27.
    Lou H, McCullough AJ, Schuler MA. 3′ splice site selection in dicot plant nuclei is position dependent. Mol Cell Biol 1993; 13:4485–4493.PubMedGoogle Scholar
  28. 28.
    Hunt AG, Mogen BD, Chu NM, Chua NH. The SV40 small t intron is accurately and efficiently spliced in tobacco cells. Plant Mol Biol 1991; 16:375–379.PubMedCrossRefGoogle Scholar
  29. 29.
    Waigmann E, Barta A. Processing of chimeric introns in dicot plants: evidence for a close cooperation between 5′ and 3′ splice sites. Nucl Acids Res 1992; 20:75–81.PubMedCrossRefGoogle Scholar
  30. 30.
    Simpson CG, Brown JWS. Efficient splicing of an AU-rich antisense intron sequence. Plant Mol Biol 1993; 21:205–211.PubMedCrossRefGoogle Scholar
  31. 31.
    Luehrsen KR, Walbot V. Insertion of nonintron sequence into maize introns interferes with splicing. Nucl Acids Res 1992; 20:5181–5187.PubMedCrossRefGoogle Scholar
  32. 32.
    Weil CF, Wessler SR. The effects of plant transposable element insertion on transcription initiation and RNA processing. Annu Rev Plant Physiol Plant Mol Biol 1990; 41:427–52.CrossRefGoogle Scholar
  33. 33.
    Nash J, Luehrsen KR, Walbot V. Bronze-2 gene of maize: Reconstruction of a wildtype allele and analysis of transcription and splicing. Plant Cell 1990; 2:1039–1049.PubMedGoogle Scholar
  34. 34.
    Wessler S. The maize transposable Ds1 element is alternatively spliced from exon sequences. Moll Cell Biol 1991; 11: 6192–6196.Google Scholar
  35. 35.
    Varagona MJ, Purugganan M, Wessler SR. Alternative splicing induced by insertion of retrotransposons into the maize waxy gene. Plant Cell 1992; 4:811–820.PubMedGoogle Scholar
  36. 36.
    Okagaki RJ, Sullivan TD, Schiefelbein JW, Nelson OE Jr. Alternative 3′ splice acceptor sites modulate enzymic activity in derivative alleles of the maize bronze1 -mutable13 allele. Plant Cell 1992; 4:1453–1462.PubMedGoogle Scholar
  37. 37.
    Mount SM, Burks C, Hertz G, Stormo GD, White O, Fields C. Splicing signals in Drosophila: intron size, information content, and consensus sequences. Nucl Acids Res 1992; 20:4255–4262.PubMedCrossRefGoogle Scholar
  38. 38.
    Conrad R, Liou RF, Blumenthal T. Functional analysis of a C. elegans trans-splice acceptor. Nucl Acids Res 1993; 21:913–919.PubMedCrossRefGoogle Scholar
  39. 39.
    Conrad R, Liou RF, Blumenthal T. Conversion of trans-spliced C. elegans gene into a conventional gene by introduction of a splice donor site. EMBO J 1993; 12: 1249–1255.PubMedGoogle Scholar
  40. 40.
    McCullough AJ, Schuler MA. AU-rich intronic elements affect pre-mRNA 5′ splice site selection in Drosophila melanogaster. Mol Cell Biol 1993; 13:7689–7697.PubMedGoogle Scholar
  41. 41.
    Copertino DW, Hallick RB. Group II and group III introns of twintrons: potential relationships with nuclear pre-mRNA introns. Trends Biochem Sci 1993; 18: 467–471.PubMedCrossRefGoogle Scholar
  42. 42.
    Werneke JM, Chatfield JM, Ogren WL. Alternative mRNA slicing generates the two ribulosebisphosphate carboxylase/oxygenase activase polypeptides in spinach and Arabidopsis. Plant Cell 1989; 1:815–825.PubMedGoogle Scholar
  43. 43.
    Rundle SJ, Zielinski RE. Alterations in barley ribulose-1, 5-bisphosphate carboxylase/oxygenase activase gene expression during development and in response to illumination. J Biol Chem 1991; 266: 14802–14807.PubMedGoogle Scholar
  44. 44.
    Hirose T, Sugita M, Sugiura M. cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurence of tissue-specific alternative splicing. Nucl Acids Res 1993; 21:3981–3987.PubMedCrossRefGoogle Scholar
  45. 45.
    Masson P, Rutherford G, Banks JA, Fedoroff N. Essential large transcripts of maize Spm transposable element are generated by alternative splicing. Cell 1989; 58:755–765.PubMedCrossRefGoogle Scholar
  46. 46.
    Accotto GP, Donson J, Mullineaux PM. Mapping of Digitara streak virus transcripts reveals different RNA splices from the same transcription unit. EMBO J 1989; 8: 1033–1039.PubMedGoogle Scholar
  47. 47.
    Schalk HJ, Matzeit V, Schiller B, Schell J, Gronenborn B. Wheat dwarf virus, a geminivirus of graminaceous plants needs splicing for replication. EMBO J 1989; 8:359–364.PubMedGoogle Scholar
  48. 48.
    Fütterer J, Potrykus I, Valles Brau MP, Dasgupta I, Hull R, Hohn T. Splicing in a plant pararetrovirus. Virology 1994; 198:663–676.PubMedCrossRefGoogle Scholar
  49. 49.
    Nash J, Walbot V. Bronze-2 gene expression and intron splicing patterns in cells and tissues of Zea mays L. Plant Plhysiol 1992; 100:464–471.CrossRefGoogle Scholar
  50. 50.
    Christensen AH, Sharrock RA, Quail PH. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol Biol 1992; 18:675–689.PubMedCrossRefGoogle Scholar
  51. 51.
    Osteryoung KW, Sundberg H, Vierling E. Poly(A) tail length of a heat shock protein RNA is increased by severe heat stress, but intron splicing is unaffected. Mol Gen Genet 1993; 239:323–333.PubMedCrossRefGoogle Scholar
  52. 52.
    Czarnecka E, Nagao RT, Key JL, Gurley WB. Characterization of Gmhsp26-A, a stress gene encodidng a divergent heat shock protein of soybean: heavy-metal-induced inhibition of intron processing. Mol Cell Biol 1988; 8:1113–1122.PubMedGoogle Scholar
  53. 53.
    Ortiz DF, Strommer JN. The Mul maize transposable element induces tissue-specific aberrant splicing and polyadenylation in two Adhi mutants. Mol Cell Biol 1990; 10:2090–2095.PubMedGoogle Scholar
  54. 54.
    Callis J, Fromm M, Walbot V. Introns increase gene expression in cultured maize cells. Genes Dev 1987; 1:1183–1200.PubMedCrossRefGoogle Scholar
  55. 55.
    Vasil V, Clancy M, Ferl RJ, Vasil IK, Hannah LC. Increased gene expression by the first intron of maize Shrunken-1 locus in grass species. Plant Physiol 1989; 91: 1575–1579.PubMedCrossRefGoogle Scholar
  56. 56.
    Maas C, Laufs J, Grant S, Korfhage C, Werr W. The combination of a novel stimulatory element in the first exon of the maize Shrunken-1 gene with the following intron 1 enhances reporter gene expression up to 1000-fold. Plant Mol Biol 1991; 16: 199–207.PubMedCrossRefGoogle Scholar
  57. 57.
    Tanaka A, Satoru M, Shozo O, Kyozuka J, Shimamoto K, Nakamura K. Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron. Nucl Acids Res 1990; 18:6767–6770.PubMedCrossRefGoogle Scholar
  58. 58.
    Norris SR, Meyer SE, Callis J. The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression. Plant Mol Biol 1993; 21:895–906.PubMedCrossRefGoogle Scholar
  59. 59.
    Luehrsen KR, Walbot V. Intron enhancement of gene expression and the splicing efficiency of introns in maize cells. Mol Gen Genet 1991; 225:81–93.PubMedCrossRefGoogle Scholar
  60. 60.
    Purugganan MD. Transposable elements as introns: evolutionary connections. Trends Ecol Evol 1993. 8:239–243.PubMedCrossRefGoogle Scholar
  61. 61.
    Menssen A, Höhmann S, Martin W, Schnable PS, Peterson PA, Saedler H, Gierl A. The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. EMBO J 1990; 9:3051–3057.PubMedGoogle Scholar
  62. 62.
    Rymond BC, Rosbash M. Yeast pre-mRNA splicing. In: Jones EW, Pringle JR, Broach JR, eds. In The Molecular and Cellular Biology of the Yeast Saccharomyces. Cold Spring Harb Lab Press 1992; 143–192.Google Scholar
  63. 63.
    White O, Soderlund C, Shanmugan P, Fields C. Information contents and dinucleotide compositions of plant intron sequences vary with evolutionary origin. Plant Mol Biol 1992; 19:1057–1064.PubMedCrossRefGoogle Scholar
  64. 64.
    Liu HX, Goodall G, Kole R, Filipowicz W. Effects of secondary structure on premRNA splicing: hairpins sequestering the 5′ but not the 3′ splice site inhibit intron processing in Nicotiana plumbaginifolia. EMBO J 1995; in press.Google Scholar

Copyright information

© R.G. Landes Company and Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • Witold Filipowicz
  • Marek Gniadkowski
  • Ueli Klahre
  • Hong-Xiang Liu

There are no affiliations available

Personalised recommendations