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Cerebellar Grafting and the Recovery of Function

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Neural Transplantation in Cerebellar Ataxia

Part of the book series: Neuroscience Intelligence Unit ((NIU.LANDES))

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Abstract

Evidence for functional recovery after cerebellar transplantation has been obtained in the pcd mouse model of hereditary cerebellar ataxia.1–4 Grafts of embryonic day 11–12 (E11–E12) cerebellar cell suspensions were placed bilaterally into the deep cerebellar nuclei of the host mutants, according to the protocol that places emphasis on reconstructing the corticonuclear γ-aminobutyric acid (GABA)ergic projection.5 Vehicle-injected pcd homozygotes were used as controls in the behavioral studies. Animals were tested in a battery of motor tasks six weeks postoperatively to determine the recovery of behavioral responses. Surviving Purkinje cells immunoreactive for 28 kDa Ca2+-binding protein (CaBP) were found in all graft-recipient animals. Counts of CaBP-immunoreactive neurons in histochemical preparations of the transplanted cerebella, combined over both sides, yielded numbers in the range of 1000–6500 surviving Purkinje cells per animal, with a 2865 cell average.2

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Authors and Affiliations

  1. Indiana University School of Medicine, Indianapolis, Indiana, USA

    Lazaros C. Triarhou M.D., Ph.D.

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  1. Lazaros C. Triarhou M.D., Ph.D.
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© 1997 Springer-Verlag Berlin Heidelberg

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Triarhou, L.C. (1997). Cerebellar Grafting and the Recovery of Function. In: Neural Transplantation in Cerebellar Ataxia. Neuroscience Intelligence Unit. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-22213-3_8

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  • DOI: https://doi.org/10.1007/978-3-662-22213-3_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-22215-7

  • Online ISBN: 978-3-662-22213-3

  • eBook Packages: Springer Book Archive

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