Abstract
During a study of fixation of bacteria for sectioning and electron microscopy using the conventional methacrylate embedding technique (1) a considerable number of blocks showing almost a 100 % burst and swollen cells were noted. By carefully controlling each step in the procedure by means of light microscopy it could be demonstrated that the swelling and bursting occurred during the actual hardening of the methacrylate (2).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Newman, S. B., E. Borysko and M. Swerdlow: J. Res. nat. Bur. Standards 43, 183 (1949).
Maaløe, O., and A. Birch-Andersen: Bacterial Anatomy, 6th Symposium of the Society for General Microbiology, p. 261. Cambridge: University Press. 1956.
Borysko, E., and P. Sapranauskas: Johns Hopk. Hosp. Bull. 95, 68 (1954).
Weinreb, S.: Science 121, 774 (1955).
Borysko, E.: J. biophys. biochem. Cytol. Suppl. 2, 3 (1956).
Birbeck, M. S. C., and E. H. Mercer: J. roy. Microscopical Soc., Ser. III 76, 159 (1956), published April 1958.
Kellenberger, E., W. Schwab et A. Ryter: Experientia (Basel) 12, 421 (1956).
Glauert, A. M., and E. M. Brieger: Proceedings of the Stockholm Conference on Electron Microscopy 111 (1956).
Glauert, A. M., G. E. Rogers and R. H. Glauert: Nature (Lond.) 178, 803 (1956).
Glauert, A. M., and R. H. Glauert: J. biophys. biochem. Cytol. 4, 191 (1958).
Peters, D., G. Nielsen and K. H. Andres: 7 th International Congress for Microbiology. Abstracts of communications 245 (1958).
Huxley, H. E.: Paper at 15th meeting of electron microscopy society of America, Boston 1957.
Robertson, J. D.: J. biophys. biochem. Cytol. 4, 39 (1958).
Ryter, A., et E. Kellenberger: Z. f. Naturforsch. 13 b, 597 (1958).
Maaløe, O., A. Birch-Andersen and F. S. Sjöstrand: Biochim. biophys. Acta 15, 12 (1954).
Kellenberger, E., and A. Ryter: J. biophys. biochem. Cytol. 4, 323 (1958).
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1960 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Birch-Andersen, A. (1960). The use of epoxy resins as embedding media for electron microscopy. In: Bargmann, W., Peters, D., Wolpers, C. (eds) Verhandlungen Band II / Biologisch-Medizinischer Teil. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-22058-0_8
Download citation
DOI: https://doi.org/10.1007/978-3-662-22058-0_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-22059-7
Online ISBN: 978-3-662-22058-0
eBook Packages: Springer Book Archive