The use of epoxy resins as embedding media for electron microscopy

  • A. Birch-Andersen
Conference paper


During a study of fixation of bacteria for sectioning and electron microscopy using the conventional methacrylate embedding technique (1) a considerable number of blocks showing almost a 100 % burst and swollen cells were noted. By carefully controlling each step in the procedure by means of light microscopy it could be demonstrated that the swelling and bursting occurred during the actual hardening of the methacrylate (2).


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Newman, S. B., E. Borysko and M. Swerdlow: J. Res. nat. Bur. Standards 43, 183 (1949).CrossRefGoogle Scholar
  2. 2.
    Maaløe, O., and A. Birch-Andersen: Bacterial Anatomy, 6th Symposium of the Society for General Microbiology, p. 261. Cambridge: University Press. 1956.Google Scholar
  3. 3.
    Borysko, E., and P. Sapranauskas: Johns Hopk. Hosp. Bull. 95, 68 (1954).Google Scholar
  4. 4.
    Weinreb, S.: Science 121, 774 (1955).PubMedCrossRefGoogle Scholar
  5. 5.
    Borysko, E.: J. biophys. biochem. Cytol. Suppl. 2, 3 (1956).CrossRefGoogle Scholar
  6. 6.
    Birbeck, M. S. C., and E. H. Mercer: J. roy. Microscopical Soc., Ser. III 76, 159 (1956), published April 1958.CrossRefGoogle Scholar
  7. 7.
    Kellenberger, E., W. Schwab et A. Ryter: Experientia (Basel) 12, 421 (1956).CrossRefGoogle Scholar
  8. 8.
    Glauert, A. M., and E. M. Brieger: Proceedings of the Stockholm Conference on Electron Microscopy 111 (1956).Google Scholar
  9. 9.
    Glauert, A. M., G. E. Rogers and R. H. Glauert: Nature (Lond.) 178, 803 (1956).CrossRefGoogle Scholar
  10. 10.
    Glauert, A. M., and R. H. Glauert: J. biophys. biochem. Cytol. 4, 191 (1958).PubMedCrossRefGoogle Scholar
  11. 11.
    Peters, D., G. Nielsen and K. H. Andres: 7 th International Congress for Microbiology. Abstracts of communications 245 (1958).Google Scholar
  12. 12.
    Huxley, H. E.: Paper at 15th meeting of electron microscopy society of America, Boston 1957.Google Scholar
  13. 13.
    Robertson, J. D.: J. biophys. biochem. Cytol. 4, 39 (1958).PubMedCrossRefGoogle Scholar
  14. 14.
    Ryter, A., et E. Kellenberger: Z. f. Naturforsch. 13 b, 597 (1958).Google Scholar
  15. 15.
    Maaløe, O., A. Birch-Andersen and F. S. Sjöstrand: Biochim. biophys. Acta 15, 12 (1954).PubMedCrossRefGoogle Scholar
  16. 16.
    Kellenberger, E., and A. Ryter: J. biophys. biochem. Cytol. 4, 323 (1958).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1960

Authors and Affiliations

  • A. Birch-Andersen
    • 1
  1. 1.Statens SeruminstitutCopenhagenDänemark

Personalised recommendations