A negative staining technique for high resolution of viruses

  • R. W. Horne
  • S. Brenner
Conference paper

Abstract

The aim of this study is to discuss the possibility of using negative staining techniques on unsectioned material. During investigations concerned with the detailed structure of the protein components of bacteriophage, serious limitations were encountered using existing shadowcasting and sectioning techniques. Fig. 1. Intact T2 phage particles in PTA droplet. Striations along the tail are clearly visible. No staining of the protein is evident

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References

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    Cosslett, V. E., and R. W. Horne : Vacuum 5, 109 (1955).CrossRefGoogle Scholar
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    Hall, C. E.: J. biophys. biochem. Cytol. 1, 1 (1955).PubMedCrossRefGoogle Scholar
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    Huxley, H. E.: Electron Microscopy Proc. Stockholm Conference, September 1956, p. 260.Google Scholar
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    Brenner, S.: This volume p. 621.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1960

Authors and Affiliations

  • R. W. Horne
    • 1
  • S. Brenner
    • 1
  1. 1.Cavendish LaboratoryUniversity of CambridgeEngland

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