Sedimentation counting of particles via electron microscopy

  • D. Gordon Sharp
Conference paper

Abstract

Electron microscope counts of virus particles have usually required highly concentrated, purified, preparations with a minimum of extraneous material (2, 4, 6). Isaacs has thoroughly reviewed these results recently (5). But, the virologist frequently does not require such purity. He generally deals with crude tissue extracts, plasmas, chorioallantoic fluids, etc., in which virus activity may be 106 to 109 ID50 per ml. It is rarely greater than 1010. This paper will describe electron microscope counting of two viruses in just such crude form.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1960

Authors and Affiliations

  • D. Gordon Sharp
    • 1
  1. 1.Department of Bacteriology, School of MedicineUniv. of North CarolinaChapel HillUSA

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