Detection of Human Hematopoietic Stem Cells in SCID-hu Mice
Totipotential hematopoietic stem cells (HSC) have an extremely high proliferative potential, therefore they can differentiate into all hematopoietic lineages, generating a high number of hematopoietic progenitors and providing a long-lasting supply of blood-borne cells.1–3 Thus, a number of in vitro assays identify HSC on the basis of their capacity to derive multiple hematopoietic lineages and of their high proliferative potential in culture (reviewed in ref. 4). It is however commonly accepted that in vitro assays do not accurately reflect the global and physiological development of hematopoiesis and thus are not ideal for preclinical testing. Research on murine HSC has strongly benefited from in vivo experimentation which is not possible with human HSC for ethical reasons. To overcome these limitations, several xenogeneic models have been designed to support the in vivo development of human hematopoietic cells in surrogate animals.
KeywordsSCID Mouse Adult Bone Marrow High Proliferative Potential Fetal Thymus Human Hematopoietic Stem Cell
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