Detection of Human Hematopoietic Stem Cells in SCID-hu Mice
Totipotential hematopoietic stem cells (HSC) have an extremely high proliferative potential, therefore they can differentiate into all hematopoietic lineages, generating a high number of hematopoietic progenitors and providing a long-lasting supply of blood-borne cells.1–3 Thus, a number of in vitro assays identify HSC on the basis of their capacity to derive multiple hematopoietic lineages and of their high proliferative potential in culture (reviewed in ref. 4). It is however commonly accepted that in vitro assays do not accurately reflect the global and physiological development of hematopoiesis and thus are not ideal for preclinical testing. Research on murine HSC has strongly benefited from in vivo experimentation which is not possible with human HSC for ethical reasons. To overcome these limitations, several xenogeneic models have been designed to support the in vivo development of human hematopoietic cells in surrogate animals.
KeywordsLymphoma Neutropenia Myeloma Sorting Etoposide
Unable to display preview. Download preview PDF.
- 6.Hendrickson EA. The SCID mouse: Relevance as an animal model system for studying human disease. Amer J Pathol 1993; 143: 1511–22.Google Scholar
- 28.DiGiusto D, Chen S, Combs J et al. Human fetal bone marrow early progenitors for T, B and myeloid cells are found exclusively in the population expressing high levels of CD34. Blood 1994; 84: 431–32.Google Scholar
- 30.Chen BP, Galy A, Kyoizumi S et al. Engraftment of human hematopoietic precursor cells with secondary transfer potential in SCID-hu mice. Blood 1994; 2497–2505.Google Scholar
- 32.Shah VO, Safford MG, Terstappen LWMM et al. Quantitative comparison of myeloid antigens on peripheral blood lymphocytes, monocytes, neutrophils, eosinophils and basophils. In: Leukocyte Typing IV. White Cell Differentiation Antigens. Knapp W, Dorken B, Gilks WR, Rieber EP, Schmidt RE, Stein H, von dem Borne AEG ed(s). Oxford: Oxford University Press, 1989; 855–58.Google Scholar
- 35.Le Douarin N, Jotereau FV. Origin and renewal of lymphocytes in avian embryo thymuses studied in interspecific combinations. Nature New Biol 1973; 246: 25–30.Google Scholar
- 48.Civin CI, Strauss LC, Brovall C et al. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-la cells. J Immunol 1984; 133: 57.Google Scholar
- 59.To LB, Roberts MM, Haylock DN et al. Comparison of haematological recovery times and supportive care requirements of autologous recovery phase peripheral blood stem cell transplants, autologous bone marrow transplants and allogeneic bone marrow transplants. Bone Marrow Transplant 1992; 9: 277–84.PubMedGoogle Scholar
- 62.Galy AHM, Cen D, Travis M. et al. Delination of T progenitor cell activity within the CD34` compartment of adullr bone marrow. Blood 1995; in press.Google Scholar