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The Cell Nucleus: Structure and Main Functions

  • Oriol Bachs
  • Neus Agell
Part of the Molecular Biology Intelligence Unit book series (MBIU)

Abstract

The nucleus is the main repository of genetic information in the eukaryotic cells and also the place where the primary genomic functions, i.e., DNA replication, transcription and RNA splicing and processing, are carried out. The numerous descriptive electron microscope and confocal microscope studies, together with the development of immunocytochemical methods and the DNA recombinant technologies has fueled a renaissance in the quest to understand the molecular organization of the cell nucleus.1

Keywords

Nuclear Envelope Nuclear Matrix Nuclear Pore Complex Nuclear Periphery Nuclear Body 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Spector DL. Macromolecular domains within the cell nucleus. Ann Rev Cell Biol 1993; 9: 265–315.PubMedCrossRefGoogle Scholar
  2. 2.
    Goldberg MW, Allen TD. Structural and functional organization of the nuclear envelope. Curr Opin Cell Biol 1995; 7: 301–309.PubMedCrossRefGoogle Scholar
  3. 3.
    Gerace L, Foisner R. Integral membrane proteins and dynamic organisation of the nuclear envelope. Trends Cell Biol 1994; 4: 127–131.PubMedCrossRefGoogle Scholar
  4. 4.
    Hinshaw JE, Carragher BO, Milligan RA. Architecture and design of the nuclear pore complex. Cell 1992; 69: 1133–1141.PubMedCrossRefGoogle Scholar
  5. 5.
    Goldberg MW, Allen TD. High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores. J Cell Biol 1992; 119: 1429–1440.PubMedCrossRefGoogle Scholar
  6. 6.
    Worman HJ, Evans CD, Blobel G. The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains. J Cell Biol 1990; 111: 1535–1542.PubMedCrossRefGoogle Scholar
  7. 7.
    Nelson WG, Pienta KJ, Barrack ER, Coffey DS. The role of nuclear matrix in the organization and function of DNA. Ann Rev Biophys Chem 1986; 15: 457–475.CrossRefGoogle Scholar
  8. 8.
    Berezney R, Coffey DS. Nuclear matrix. Isolation and characterization of a framework structure from rat liver nuclei. J Cell Biol 1977; 73: 616–637.PubMedCrossRefGoogle Scholar
  9. 9.
    Hakes DJ, Berezney R. DNA binding properties of the nuclear matrix and individual nuclear matrix proteins. J Biol Chem 1991; 266: 11131–11140.PubMedGoogle Scholar
  10. 10.
    Tubo RA, Berezney R. Pre-replicative association of multiple replicative enzyme activities with the nuclear matrix during rat liver regeneration. J Biol Chem 1987; 269: 1148–1154.Google Scholar
  11. 11.
    Jaumot M, Grana X, Giordano A, Reddy PGV, Agell N, Bachs O. Cyclin/Cdk2 complexes in the nucleus of HeLa cells. Biochem Biophys Res Commun 1994; 203: 1527–1534.PubMedCrossRefGoogle Scholar
  12. 12.
    Grana X, Reddy EP. Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs). Oncogene 1995; in press.Google Scholar
  13. 13.
    Thompson NL, Mead JE, Braun L, Goyette M, Shank PR, Fausto N. Sequential protooncogene expression during rat liver regeneration. Cancer Res 1986; 46: 3111–3117.PubMedGoogle Scholar
  14. 14.
    Serratosa J, Pujol MJ, Bachs O, Carafoli E. Rearrangement of nuclear calmodulin during proliferative liver cell activation. Biochem Biophys Res Commun 1988; 150: 1162–1169.PubMedCrossRefGoogle Scholar
  15. 15.
    Jackson DA, Cook PR. Transcription occurs at a nucleoskeleton. EMBO J 1985; 4: 919–925.PubMedGoogle Scholar
  16. 16.
    Brasch K, Ochs RL. Nuclear bodies (NBs): a newly “rediscovered” organelle. Exp Cell Res 1992; 202: 211–223.PubMedCrossRefGoogle Scholar
  17. 17.
    Ferguson M, Ward DC. Cell cycle dependent chromosomal movement in pre-mitotic human T-lymphocyte nuclei. Chromosoma 1992; 101: 557–565.PubMedCrossRefGoogle Scholar
  18. 18.
    Fakan S, Puvion E, Spohr G. Localization and characterization of newly synthesized nuclear RNA in isolated rat hepatocytes. Exp Cell Res 1976; 99: 155–164.PubMedCrossRefGoogle Scholar
  19. 19.
    Spector DL, Fu XD, Maniatis T. Associations between distinct premRNA splicing components and the cell nucleus. EMBO J 1991; 10: 3467–3481.PubMedGoogle Scholar
  20. 20.
    Cook PR. The nucleoskeleton and the topology of replication. Cell 1991; 66: 627–635.PubMedCrossRefGoogle Scholar
  21. 21.
    Hadjiolov A. The nucleolus and ribosome biogenesis. Cell Biol Mono 1985; 12: 1–263.CrossRefGoogle Scholar
  22. 22.
    Raska I, Ochs RL, Salamin-Michel L. Immunocytochemistry of the cell nucleus. Electron Micros Rev 1990; 3: 301–353.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Oriol Bachs
    • 1
  • Neus Agell
    • 1
  1. 1.University of BarcelonaBarcelonaSpain

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