Abstract
Alterations in microcirculatory permeability are characteristic of early tissue injury, and are thought to result from contraction of activated endothelial cells (Fig. 1). A defining hallmark of a number of inflammatory conditions including sepsis and the acute respiratory distress syndrome (ARDS), is the so-called high-permeability interstitial edema of the systemic and pulmonary circulations, respectively [1]. Moreover, tissue edema is an early indicator of tissue dysfunction, prior to organ failure [2]. The extent of edema formation has been associated with impaired gas exchange and arterial hypoxemia in ARDS. Edema may also impair tissue oxygen distribution due to increased intercapillary distances, such that speculation regarding its potential role in exacerbating tissue hypoxia persists despite evidence to suggest a lack of external compression of skeletal muscle microvessels to account for increased capillary density in a rodent model of sepsis [3]. As a result of increased microvascular permeability, the loss of plasma fluid to the interstitial space leads to hypovolemia, with increased sympathetic activation and an imbalance of fluid/electrolyte homeostasis. This exacerbates the persisting hemodynamic instability. Currently, no proven effective pharmacologic therapy is available to reduce increased permeability, although numerous agents are under investigation. Such a drug, with a capillary-permeability reducing effect, would clearly be of great value in the critically ill. The following chapter briefly reviews theories of permeability to macromolecules (i.e., protein), the mediators that bring about these changes, and the mechanisms by which these processes are postulated to occur, in the context of acute inflammation, particularly during sepsis. For a detailed review of theoretical aspects of microvascular permeability, the reader is directed to a recent authoritative text on the subject [4].
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References
Bernard GR, Artigas A, Brigham KL, et al (1994) The American-European Consensus Conference on ARDS. Definitions, mechanisms, relevant outcomes, and clinical trial coordination. Am J Respir Crit Care Med 149: 818–824
Nieuwenhuijzen GA, Knapen MF, Oyen WJ, Hendriks T, Corstens FH, Goris RJ (1997) Organ damage is preceded by changes in protein extravasation in an experimental model of multiple organ dysfunction syndrome. Shock 7: 98–104
Piper RD, Pitt-Hyde M, Li F, Sibbald WJ, Potter RF (1996) Microcirculatory changes in rat skeletal muscle in sepsis. Am J Respir Crit Care Med 154: 931–937
Michel CC, Curry FE (1999) Microvascular permeability. Physiol Rev 79: 703–761
Singh S, Evans TW (1997) Nitric oxide, the biological mediator of the decade: fact or fiction? Eur Respir J 10: 699–707
Starling E (1896) On the absorption of fluids from the connective tissue spaces. J Physiol London 19: 312–326
Grotte G (1956) Passage of Dextran molecules across the blood lymph barrier. Acta Chur Scand Suppl 211: 1–84
Winlove CP, Parker KH (1993) Vascular biophysics: mechanics and permeability. Eur Respir Rev 3: 535–542
Baldwin GS, Kelly SM, Price NC, et al (1994) Ligand-induced conformational states of the cytosine-specific DNA methyltransferase M.HgaI-2. J Mol Biol 235: 545–553
Merkle CJ, Wilson LM, Baldwin AL (1998) Acute blood stasis reduces interstitial uptake of albumin from intestinal microcirculatory networks. Am J Physiol 274: H600 - H608
Renkin EM, Tucker V, Rew K, O’Loughlin D, Wong M, Sibley L (1992) Plasma volume expansion with colloids increases blood-tissue albumin transport. Am J Physiol 262: H1054 - H1067
Yuan Y, Granger HJ, Zawieja DC, Chilian WM (1992) Flow modulates coronary venular permeability by a nitric oxide-related mechanism. Am J Physiol 263: H641 - H646
van Lambalgen AA, van den Bos GC, Thijs LG (1987) Changes in regional plasma extravasation in rats following endotoxin infusion. Microvasc Res 34: 116–132
Vehaskari VM, Chang CT, Stevens JK, Robson AM (1984) The effects of polycations on vascular permeability in the rat. A proposed role for charge sites. J Clin Invest 73: 1053–1061
Shostak A, Gotloib L (1998) Increased mesenteric, diaphragmatic, and pancreatic interstitial albumin content in rats with acute abdominal sepsis. Shock 9: 135–137
Haupt MT (1989) The use of crystalloidal and colloidal solutions for volume replacement in hypovolemic shock. Crit Rev Clin Lab Sci 27: 1–26
Gamble J, Gartside IB, Christ F (1993) A reassessment of mercury in silastic strain gauge plethysmography for microvascular permeability assessment in man. J Physiol (Lond) 464: 407–422
Mayhan WG (1992) Role of nitric oxide in modulating permeability of hamster cheek pouch in response to adenosine 5’ -diphosphate and bradykinin. Inflammation 16: 295–305
Hunter DN, Lawrence R, Morgan CJ, Evans TW (1990) The use of caesium iodide mini scintillation counters for dual isotope pulmonary capillary permeability studies. Nucl Med Commun 11: 879–888
Smith G, Weidel SE, Fleck A (1994) Albumin catabolic rate and protein-energy depletion. Nutrition 10: 335–341
Deng X, Wang X, Andersson R (1995) Endothelial barrier resistance in multiple organs after septic and nonseptic challenges in the rat. J Appl Physiol 78: 2052–2061
Grega GJ, Adamski SW, Dobbins DE (1986) Physiological and pharmacological evidence for the regulation of permeability. Fed Proc 45: 96–100
Majno G, Shea SM, Leventhal M (1969) Endothelial contraction induced by histamine-type mediators: an electron microscopic study. J Cell Biol 42: 647–672
Singh S, Anning PB, Winlove CP, Evans TW (1999) Permeability of aortic endothelium to albumin is altered by sepsis and L-NAME. Am J Respir Crit Care Med 159 (Suppl 3 ): A613 (abst)
Arfors KE, Rutili G, Svensjo E (1979) Microvascular transport of macromolecules in normal and inflammatory conditions. Acta Physiol Scand Suppl 463: 93–103
Bent-Shansen L (1991) Whole capillary body exchange of albumin Acta Physiol Scand 143: 5–10
Lonigro AJ, McMurdo L, Stephenson AH, Sprague RS, Weintraub NL (1996) Hypotheses regarding the role of pericytes in regulating movement of fluid, nutrients, and hormones across the microcirculatory endothelial barrier. Diabetes 45 (Suppl 1): S38 - S43
Braverman IM, Keh-Yen A (1986) Three-dimensional reconstruction of endothelial cell gaps in psoriatic vessels and their morphologic identity with gaps produced by the intradermal injection of histamine. J Invest Dermatol 86: 577–581
Mayerson H, Wolfram CG, Shirley HH, Wasserman K (1960) Regional differences in capillary permeability. Am J Physiol 198: 155–160
Palade G (1960) Transport in quanta across the endothelium of blood capillaries. Anat Rec 136: 254–271
Wagner RC, Chen SC (1991) Transcapillary transport of solute by the endothelial vesicular system: evidence from thin serial section analysis. Microvasc Res 42: 139–150
Feng D, Nagy JA, Hipp J, Pyne K, Dvorak HF, Dvorak AM (1997) Reinterpretation of endothelial cell gaps induced by vasoactive mediators in guinea-pig, mouse and rat: many are transcellular pores. J Physiol (Lond) 504: 747–761
Schnitzer JE, Allard J, Oh P (1995) NEM inhibits transcytosis, endocytosis, and capillary permeability: implication of caveolae fusion in endothelia. Am J Physiol 268: H48 - H55
Michel CC (1996) Transport of macromolecules through microvascular walls. Cardiovasc Res 32: 644–653
Granger DN, Kubes P (1994) The microcirculation and inflammation: modulation of leukocyte-endothelial cell adhesion. J Leukoc Biol 55: 662–675
Kurose I, Kubes P, Wolf R, et al (1993) Inhibition of nitric oxide production. Mechanisms of vascular albumin leakage. Circ Res 73: 164–171
Panes J, Perry MA, Anderson DC, et al (1995) Regional differences in constitutive and induced ICAM-1 expression in vivo. Am J Physiol 269: H1955 - H1964
Chen K, Inoue M, Okada A (1996) Expression of inducible nitric oxide synthase mRNA in rat digestive tissues after endotoxin and its role in intestinal mucosal injury. Biochem Biophys Res Commun 224: 703–708
Kurose I, Suematsu M, Miura S, et al (1993) Oxyradical generation from leukocytes during endotoxin-induced microcirculatory disturbance in rat mesentery–attenuating effect of cetraxate. Toxicol Appl Pharmacol 120: 37–44
Seiffge D, Bissinger T, Kremer E, Laux V, Schleyerbach R (1995) Inhibitory effects of pentoxifylline on LPS-induced leukocyte adhesion and macromolecular extravasation in the microcirculation. Inflamm Res 44: 281–286
Schmidt W, Schmidt H, Bauer H, Gebhard MM, Martin E (1997) Influence of lidocaine on endotoxin-induced leukocyte-endothelial cell adhesion and macromolecular leakage in vivo. Anesthesiology 87: 617–624
Schmidt H, Schmidt W, Muller T, Bohrer H, Gebhard MM, Martin E (1997) N-acetylcysteine attenuates endotoxin-induced leukocyte-endothelial cell adhesion and macromolecular leakage in vivo. Crit Care Med 25: 858–863
Schmidt H, Ebeling D, Bauer H, Bohrer H, Gebhard MM, Martin E (1996) Influence of the platelet-activating factor receptor antagonist BN52021 on endotoxin-induced leukocyte adherence in rat mesenteric venules. J Surg Res 60: 29–35
Kubes P, Granger DN (1992) Nitric oxide modulates microvascular permeability. Am J Physiol 262: H611 - H615
Mayhan WG (1994) Nitric oxide accounts for histamine-induced increases in macromolecular extravasation. Am J Physiol 266: H2369 - H2373
Hutcheson IR, Whittle BJ, Boughton-Smith NK (1990) Role of nitric oxide in maintaining vascular integrity in endotoxin-induced acute intestinal damage in the rat. Br J Pharmacol 101: 815–820
Laszlo F, Whittle BJ, Moncada S (1995) Attenuation by nitrosothiol NO donors of acute intestinal microvascular dysfunction in the rat. Br J Pharmacol 115: 498–502
Felipe J, Delalandre A, Beauchamp M (1997) A dual role for nitric oxide in the regulation of plasma volume and albumin escape during endotoxin shock in conscious rats. Circ Res 81: 840–847
Arkovitz MS, Wispe JR, Garcia VF, Szabo C (1996) Selective inhibition of the inducible isoform of nitric oxide synthase prevents pulmonary transvascular flux during acute endotoxemia. J Pediatr Surg 31: 1009–1015
Mikawa K, Nishina K, Tamada M, Takao Y, Maekawa N, Obara H (1998) Aminoguanidine attenuates endotoxin-induced acute lung injury in rabbits. Crit Care Med 26: 905–911
Laszlo F, Whittle BJ (1997) Actions of isoform-selective and non-selective nitric oxide synthase inhibitors on endotoxin-induced vascular leakage in rat colon. Eur J Pharmacol 334: 99–102
Garvey EP, Oplinger JA, Furfine ES, et al (1997) 1400 W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase in vitro and in vivo. J Biol Chem 272: 4959–4963
Hickey MJ, Sharkey KA, Sihota EG, et al (1997) Inducible nitric oxide synthase-deficient mice have enhanced leukocyte-endothelium interactions in endotoxemia. Faseb J 11: 955–964
Boughton-Smith NK, Evans SM, Whittle BJ, Moncada S (1993) Induction of nitric oxide synthase in rat intestine and its association with tissue injury. Agents Actions 38: C125 - C126
Singh S, Anning PB, Winlove CP, Evans TW (1998) N-nitro-L-arginine methylester (L-NAME) reduces pulmonary macromolecular transport in endotoxaemia. Am J Respir Crit Care Med 157: A677 (abst)
Suematsu M, DeLano FA, Poole D, et al (1994) Spatial and temporal correlation between leukocyte behavior and cell injury in postischemic rat skeletal muscle microcirculation. Lab Invest 70: 684–695
Duffey ME, Hainau B, Ho S, Bentzel CJ (1981) Regulation of epithelial tight junction permeability by cyclic AMP. Nature 294: 451–453
Adamson RH, Liu B, Fry GN, Rubin LL, Curry FE (1998) Microvascular permeability and number of tight junctions are modulated by cAMP. Am J Physiol 274: H1885 - H1894
Xiong Z, Sperelakis N (1995) Regulation of L-type calcium channels of vascular smooth muscle cells. J Mol Cell Cardiol 27: 75–91
He P, Zeng M, Curry FE (1998) cGMP modulates basal and activated microvessel permeability independently of [Ca2 + l i. Am J Physiol 274: H1865 - H1874
Shepro D, Morel NM (1993) Pericyte physiology. Faseb J 7: 1031–1038
Groeneveld AB, den Hollander W, Straub J, Nauta JJ, Thijs LG (1990) Effects of N-acetylcysteine and terbutaline treatment on hemodynamics and regional albumin extravasation in porcine septic shock. Circ Shock 30: 185–205
Moller AD, Grande PO (1999) Low-dose prostacyclin is superior to terbutaline and aminophylline in reducing capillary permeability in cat skeletal muscle in vivo. Crit Care Med 27: 130–136
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Singh, S., Winlove, C.P., Evans, T.W. (2000). Microvascular Permeability in Experimental Sepsis: Mechanisms, Modulation and Management. In: Vincent, JL. (eds) Yearbook of Intensive Care and Emergency Medicine 2000. Yearbook of Intensive Care and Emergency Medicine, vol 2000. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-13455-9_8
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DOI: https://doi.org/10.1007/978-3-662-13455-9_8
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