Studies of Protein and Amino Acid Metabolism in the Human Liver

  • H. Barle
  • P. Essén
  • J. Wernerman
Conference paper
Part of the Yearbook of Intensive Care and Emergency Medicine book series (YEARBOOK, volume 1999)


Protein depletion is a major determinant of clinical outcome in a number of catabolic states. Hence, assessment of protein metabolism is necessary to understand these conditions as well as to evaluate treatment. Since the regulation of synthesis and breakdown of proteins differs between tissues, it is preferable to study the parameters of protein metabolism separately, and at the organ level. In this chapter we will discuss techniques developed to study liver protein metabolism in man. A technique for liver tissue sampling was established during laparoscopic surgery in order to investigate some parameters of human liver protein metabolism, free amino acid concentrations (ion exchange chromatography), and synthesis rates of total liver protein and albumin (stable isotope technique and gas chromatography-mass spectrometry (GC-MS) as well as ribosome analysis). The impact on these parameters of intravenous nutrition, of growth hormone and of the surgical procedure are also discussed.


Growth Hormone Total Parenteral Nutrition Synthesis Rate Branch Chain Amino Acid Muscle Protein Synthesis 
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© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • H. Barle
  • P. Essén
  • J. Wernerman

There are no affiliations available

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