The Prokaryotes pp 1063-1074 | Cite as

The Genus Brucella

  • Margaret E. Meyer


The genus Brucella historically has been classified in the family Brucellaceae, but it was recently removed from this family and placed with several other genera in a group of unaffiliated Gram-negative aerobes. Members of this genus are important bacteria because of the widespread and serious nature of the disease they can cause in both man and animal. Brucellosis (undulant fever) in man is incapacitating and can become chronic and lead to permanent invalidism. Brucellosis in animals is of crucial concern, particularly in the overcrowded and protein-hungry areas of the world, because it is one of the leading causes of abortion and sterility in all domesticated and semidomesticated livestock used by man for meat, milk, hair, wool, hides, fertilizer, fuel, for carrying burdens, and for tilling soil.


Brucella Abortus Brucella Species Brucella Melitensis Classical Species Direct Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Abdussalam M., Fein, D. A. 1976. Brucellosis as a world problem, pp. 9–23. In: Regamey, R. H., Hulse, E. C., Valette, L. (eds.), International Symposium on Brucellosis (II). Basel: Karger.Google Scholar
  2. Ahvonen, P., Jansson, E., Aho, K. 1969. Marked cross-agglutination between Brucellaeand a subtype of Yersinia entero-colitica. Acta Pathologica et Microbiologica Scandinavica 75:291–295.PubMedGoogle Scholar
  3. Aller, B. 1975. Brucellosis in Spain. International Journal of Zoonoses 2:10–15.PubMedGoogle Scholar
  4. Alton, G. G., Jones, M., Pietz, D. E. 1975. Bacteriological methods, pp. 9–41. In: Laboratory techniques in brucellois. World Health Organization Monograph Series No. 55. Geneva: World Health Organization.Google Scholar
  5. Angus, R. D., Brown, G. M., Gue, C. S. 1971. Avian brucellosis: A case report of natural transmission from cattle. American Journal of Veterinary Research 32:1609–1612.PubMedGoogle Scholar
  6. Angus, R. D., Love, E. L., Pietz, D. E. 1977. Evaluation of five mediums for the stabilization of Brucella abortusstrain 19 desiccated by lyophilization, pp. 307–312. In: Cabasso, V J., Regamey, R. H. (eds.), International Symposium for the Freeze-Drying of Biological Products. Basel: Karger.Google Scholar
  7. Anonymous. 1959. Chalk up ten cents per hog for brucellosis. National Hog Farmer 4:19.Google Scholar
  8. Bang, B. 1897. The etiology of epizootic abortion. Journal of Comparative Pathology 10:125–149.Google Scholar
  9. Biegeleisen, J. Z., Moody, M. D., Marcus, B. B., Flynt, J. W. 1962. The use of fluoréscein-labeled anti-Brucella suisglobulin for demonstrating Brucellaantigen in animal tissues. American Journal of Veterinary Research 23:592–595.Google Scholar
  10. Boyce, K. J., Edgar, A. W., Maley, A. D. 1967. The continuous culture of Brucella abortus(strain 19). Mitteilungen der Versuchsstation für das Gärungsgewerbe in Wein 21:122–126.Google Scholar
  11. Brodie, J., Sinton, G. P. 1975. Fluid and solid media for isolation of Brucella abortus. Journal of Hygiene, Cambridge 74:359–367.Google Scholar
  12. Brown, G. M., Ranger, C. R., Kelley, D. J. 1971. Selective media for the isolation of Brucella ovis. Cornell Veterinarian 61:265–280.PubMedGoogle Scholar
  13. Bruce, D. 1888. The micrococcus of Malta Fever. Practitioner 40:241–249.Google Scholar
  14. Bruce, M. D. 1893. Sur une nouvelle forme de fièvre. Recontrée sur les bords de la Méditerranée. Annales de 1’Institute Pasteur (Paris) 7:289–304.Google Scholar
  15. Buddle, M. B. 1956. Studies on Brucella ovis(n. sp.). A cause of genital disease of sheep in New Zealand and Australia. Journal of Hygiene 54:351–364.PubMedCrossRefGoogle Scholar
  16. Cameron, H. S. 1932. The viability of Brucella abortus. Cornell Veterinarian 22:212–224.Google Scholar
  17. Carmichael, L. E., Bruner, D. W. 1968. Characteristics of a newly recognized species of Brucellaresponsible for infectious canine abortions. Cornell Veterinarian 48:579–592.PubMedGoogle Scholar
  18. Carmichael, L. E., George, L. W. 1976. Canine brucellosis: Newer knowledge, pp. 237–247. In: Regamey, R. H., Hulse, E. C., Valette, L. (eds.), International Symposium on Brucellosis (II). Basel: Karger.Google Scholar
  19. Castenada, M. R. 1947. A practical method for routine blood cultures in brucellosis. Proceedings of the Society for Experimental Biology and Medicine 64:114–115.CrossRefGoogle Scholar
  20. Center for Disease Control. 1970. Zoonoses surveillance: Brucellosis. Atlanta, Georgia: United States Department of Health, Education, and Welfare.Google Scholar
  21. Condy, J. B., Vickers, D. B. 1969. The isolation of Brucella abortusfrom a waterbuck (Kobus ellipsiprymnus). Veterinary Record 85:200.CrossRefGoogle Scholar
  22. Corbel, M. J. 1973. Examination of two bacterial strains designated “Brucella suis”biotype 5. Journal of Hygiene, Cambridge 71:271–282.CrossRefGoogle Scholar
  23. Corbel, M. J. 1977. Production of a phage variant lytic for non-smooth Brucellastrains. Annali Sclavo 19:99–108.PubMedGoogle Scholar
  24. Corner, A. H., Connell, R. 1958. Brucellosis in bison, elk, and moose in Elk Island National Park, Canada. Canadian Journal of Comparative Medicine 22:9–20.Google Scholar
  25. Cotton, W. E. 1922. The character and possible significance of the Bang abortion bacillus that attacks swine. Journal of American Veterinary Medical Association 62:179–192.Google Scholar
  26. Diaz, R., Bosseray, N. 1973. Identification d’un composé antigénique spécifique de la phase ruguese (R) des Brucella. Annales de Recherches Vétérinaires 4:283–292.Google Scholar
  27. Diaz, R., Jones, L. M., Wilson, J. B. 1968. Antigenic relationship of the Gram-negative organism causing canine abortion to smooth and rough brucellae. Journal of Bacteriology 95:618–624.PubMedGoogle Scholar
  28. Douglas, J. T., Elberg, S. S. 1976. Isolation of Brucella melitensisphage of broad biotype and species specificity. Infection and Immunity 14:306–308.PubMedGoogle Scholar
  29. Doyle, L. P., Spray, R. S. 1920. Infectious abortion of swine. Journal of Infectious Diseases 27:165–168.Google Scholar
  30. Elberg, S. S. 1958. The Brucella, pp. 437–452. In: Dubos, R. J. (ed.), Bacterial and mycotic infections of man. Philadelphia: J. B. Lippincott.Google Scholar
  31. Evans, A. C. 1918. Further studies on Bacterium abortusand related bacteria. II. A comparison of Bacterium abortusand Bacterium bronchisepticuswith the organism which causes Malta Fever. Journal of Infectious Diseases 22:580–593.CrossRefGoogle Scholar
  32. Farrell, I. D. 1974. The development of a new selective medium for the isolation of Brucella abortusfrom contaminated sources. Research in Veterinary Science 16:280–286.PubMedGoogle Scholar
  33. Farrell, I. D., Robertson, L. 1967. The sensitivity of Brucella abortusto three antibiotics used in selective media, and the description of a new biotype. Journal of Hygiene, Cambridge 65:165–172.Google Scholar
  34. Feusier, M. L., Meyer, K. F. 1920. Principles in serologic grouping of B. abortus and B. melitensis. Correlation between absorption and agglutination tests. Studies on the genus Brucella nov. gen II. Journal of Infectious Diseases 27: 185–206.CrossRefGoogle Scholar
  35. Gerhardt, P. 1958. The nutrition of brucellae. Bacteriological Reviews 22:81–98.PubMedGoogle Scholar
  36. Gerhardt, P., Wilson, J. B. 1948. The nutrition of brucellae: Growth in simple chemically defined media. Journal of Bacteriology 56:17–24.PubMedGoogle Scholar
  37. Good, E. S., Smith, W. W. 1916. Bacillus abortus(Bang) as an etiological factor in infectious abortion in swine. Journal of Bacteriology 1:415–422.PubMedGoogle Scholar
  38. Hall, W. H., Manion, R. E. 1970. In vitro susceptibility of Brucellato various antibiotics. Applied Microbiology 20:600–604.PubMedGoogle Scholar
  39. Hatten, Betty A., Sulkin, E. S. 1968. Possible role of Brucella abortusL-forms in the pathogenesis of brucellosis, pp. 457–471. In: Guze, L. B. (ed.), Microbial protoplasts, spheroplasts, and L-forms. Baltimore: Williams & Wilkins.Google Scholar
  40. Hausier, W. J., Koontz, F. P. 1974. Brucella, pp. 295–301. In: Lennette, E. H., Spaulding, E. H., Truant, J. P. (eds.), Manual of clinical microbiology. Washington, D.C.: American Society for Microbiology.Google Scholar
  41. Hoff, G. L., Bigler, W. J., Trainer, D. O., Debbie, J. G., Brown, G. M., Winkler, W. G., Richards, S. H., Reardon, M. 1974. Survey of selected carnivore and opossum serums for agglutinins to Brucella canis. Journal American Veterinary Medical Association 165:830–831.Google Scholar
  42. Hoyer, B. H., McCullough, N. B. 1968a. Polynucleotide homologies of Brucelladeoxyribonucleic acids. Journal of Bacteriology 95:444–448.PubMedGoogle Scholar
  43. Hoyer, B. H., McCullough, N. B. 1968b. Homologies of deoxyribonucleic acids from Brucella ovis, canine abortion organisms, and other Brucellaspecies. Journal of Bacteriology 96:1783–1790.PubMedGoogle Scholar
  44. Huddleson, I. F. 1929. The differentiation of the species in the genus Brucella. Michigan State College Agricultural Experiment Station Technical Bulletin No. 100. East Lansing, Michigan: Agricultural Experiment Station, Michigan State University.Google Scholar
  45. Huddleson, I. F., Abell, E. 1927. A biochemical method of differentiating Brucella abortusfrom Brucella melitensis-paramelitensis. Journal of Bacteriology 13:13–14.Google Scholar
  46. International Committee on Nomenclature of Bacteria, Subcommittee on the Taxonomy of Brucella. 1971. Minutes of Meeting 7 August 1970. International Journal of Systematic Bacteriology 21:126–128.CrossRefGoogle Scholar
  47. Kimberling, C. H., Luchsinger, D. N., Anderson, R. K. 1966. Three cases of canine brucellosis. Journal of the American Veterinary Medical Association 148:900–901.PubMedGoogle Scholar
  48. Koser, S. A., Breslove, B. B., Dorfman, A. 1941. Accessory growth factor requirements of some representatives of the Brucella group. Journal of Infectious Diseases 69:114 – 124.CrossRefGoogle Scholar
  49. Kuzdas, C. D., Morse, E. V. 1953. A selective medium for the isolation of brucellae from contaminated materials. Journal of Bacteriology 66:502–504.PubMedGoogle Scholar
  50. Kuzdas, C. D., Morse, E. V 1954. The survival of Brucella abortus, U.S.D.A. strain 2308 under controlled conditions in nature. Cornell Veterinarian 44:216–228.PubMedGoogle Scholar
  51. Losiiiski, T., Chwalibög, J., Kempski, W., Lewkcwicz, H., Owadiuk, Z., Wisniewski, B. 1972. Studies on brucellosis in hares. [In Polish, with English summary.] Medycyna Weterynaryjna 28:331–334.Google Scholar
  52. McCullough, N. B., Dick, L. A. 1942a. Physiological studies on Brucella. I. Quantitative accessory growth factor requirements of certain strains of Brucella. Journal of Infectious Diseases 71:193–197.CrossRefGoogle Scholar
  53. McCullough, N. B., Dick, L. A. 1942b. Physiological studies of Brucella. II. Accessory growth factor requirements of recently isolated strains of Brucella abortus. Journal of Infectious Diseases 71:198–200.CrossRefGoogle Scholar
  54. McCullough, N. B., Dick, L. A. 1943. Growth of Brucellain a simple chemically defined medium. Proceedings of the Society for Experimental Biology and Medicine 52:310–311.CrossRefGoogle Scholar
  55. McCullough, W. G., Mills, R. C., Herbst, E. J., Roessler, W. G., Brewer, C. R. 1947. Studies on the nutritional requirements of Brucella suis. Journal of Bacteriology 53:5–15.Google Scholar
  56. Mammatelashvilli, N. G. 1970. Pathological changes in female buffaloes killed at various times after reinfection with Brucella. Spornik Trudov Gruzinskii 37:95–99.Google Scholar
  57. Mathur, A. C., Sharma, G. L. 1976. Studies on estimates of economic losses caused by brucellosis among bovines and its control in India. Indian Journal of Animal Science 44:654–661.Google Scholar
  58. Mathur, T. N. 1964. Brucellastrains isolated from cows, buffaloes, goats, sheep, and human beings at Karnal: Their significance with regard to the epidemiology of brucellosis. Indian Journal of Medical Research 52:1231–1240.PubMedGoogle Scholar
  59. Meyer, K. F., Shaw, E. B. 1920. A comparison of the morphologic, cultural and biochemical characteristics of B. abortusand B. melitensis. Journal of Infectious Diseases 27:173–184.CrossRefGoogle Scholar
  60. Meyer, M. E.,1961. Metabolic characterization of the genus Brucella. HI. Oxidative metabolism of strains that show anomolous characteristics by conventional determinative methods. Journal of Bacteriology 82:401–410.PubMedGoogle Scholar
  61. Meyer, M. E. 1964. The epizootiology of brucellosis and its relationship to the identification of Brucellaorganisms. American Journal of Veterinary Research 25:553–557.PubMedGoogle Scholar
  62. Meyer, M. E. 1966a. Host-parasite relationships in brucellosis. I. Reservoirs of infection and interhost transmissibility of the parasite, pp. 129–134. Proceedings United States Livestock Sanitary Association. Richmond, Virginia: Carter Composition Corp.Google Scholar
  63. Meyer, M. E. 1966b. Identification of Brucella organisms by immunofluorescence. American Journal of Veterinary Research 27:424–429.PubMedGoogle Scholar
  64. Meyer, M. E. 1969a. Phenotypic comparison of Brucella ovisto the DNA-homologous Brucellaspecies. American Journal of Veterinary Research 30:1757–1764.PubMedGoogle Scholar
  65. Meyer, M. E. 1969b. Brucellaorganisms isolated from dogs: Comparison of characteristics to members of the genus Brucella. American Journal of Veterinary Research 30:1751–1756.PubMedGoogle Scholar
  66. Meyer, M. E. 1974. Advances in research in brucellosis, pp. 231–250. In: Brandly, C. A., Cornelius, C. E. (eds.), Advances in Veterinary Science and Comparative Medicine. New York: Academic Press.Google Scholar
  67. Meyer, M. E. 1976a. Evolution and taxonomy in the genus Brucella. Brucellosis of rodents. Theriogenology 6:263–272.CrossRefGoogle Scholar
  68. Meyer, M. E. 1976b. Evolution and taxonomy in the genus Brucella: Steroid hormone induction of filterable forms with altered characteristics after reversion. American Journal of Veterinary Research 37:207–210.PubMedGoogle Scholar
  69. Meyer, M. E. 1976c. Evolution and taxonomy in the genus Brucella: Progesterone induction of filterable forms of Brucella abortus, type 2 with revertant characteristics essentially indistinguishable in vitrofrom Brucella ovis. American Journal of Veterinary Research 37:211–214.PubMedGoogle Scholar
  70. Meyer, M. E., Cameron, H. S. 1961a. Metabolic characterization of the genus Brucella. I. Statistical evaluation of the oxidative rates by which type 1 of each species can be identified. Journal of Bacteriology 82:387–395.PubMedGoogle Scholar
  71. Meyer, M. E., Cameron, H. S. 1961b. Metabolic characterization of the genus Brucella. II. Oxidative patterns of the described biotypes. Journal of Bacteriology 82:396–400.PubMedGoogle Scholar
  72. Meyer, M. E., Morgan, W. J. B. 1973. Designation of neotype strains and of biotype reference strains for species in the genus BrucellaMeyer and Shaw. International Journal of Systematic Bacteriology 23:135–141.CrossRefGoogle Scholar
  73. Moreira-Jacob, N. 1968. New group of virulent bacteriophages showing differential affinity for Brucellaspecies. Nature 219:752–753.PubMedCrossRefGoogle Scholar
  74. Morgan, W. J. B., McCullough, N. B. 1974. Genus BrucellaMeyer and Shaw, pp. 278–282. In: Buchanan, R. E., Gibbons, N. E. (eds.), Bergey’s manual of determinative bacteriology, 8th ed. Baltimore: Williams & Wilkins.Google Scholar
  75. Morris, J. A., Corbel, M. J. 1973. Properties of a new phage lytic for Brucella suis. Journal of General Virology 21:539–544.PubMedCrossRefGoogle Scholar
  76. Morris, J. A., Corbel, M. J., Phillip, J. I. H. 1973. Characterization of three new phages lytic for Brucellaspecies. Journal of General Virology 20:63–73.PubMedCrossRefGoogle Scholar
  77. Otgon, Ts. 1968. The epidemiology and epizootiology of brucellosis in Mongolia. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii 8:120–125.Google Scholar
  78. Painter, G. M., Deype, B. L., Lambert, G. 1966. Comparison of several media for the isolation of Brucella. Canadian Journal of Comparative Medicine and Veterinary Science 30:218–223.PubMedGoogle Scholar
  79. Philippon, A. 1968. Identification de Brucella abortus: Métabolisme oxidatif et lysotypie. Annales de l’Institut Pasteur 115:367–378.PubMedGoogle Scholar
  80. Pinigin, A. F. 1971. Brucellosis in Northern Reindeer. Irkutsk: Eastern Siberian Book Publishing House.Google Scholar
  81. Portugal, M., Nesti, A., Giorgi, W., Franca, E. N., de Oliviera, B. S. 1971. Brucellosis in horses caused by Brucella suis. Arquives Institute Biology (Sao Paulo) 38:125–129.Google Scholar
  82. Prichard, W. D., Hagen, K. W., Gorham, J. R., Stiles, F. C. 1971. An epizootic of brucellosis in mink. Journal of the American Veterinary Medical Association 159:635–637.PubMedGoogle Scholar
  83. Regamey, R. H., de Barbieri, A., Hennessen, W., Ikie, D., Perkins, F. T. (eds.) 1970. International Symposium on Brucellosis. Standardization and control of vaccines and reagents. Basel: Karger.Google Scholar
  84. Reports of the Commission for the Investigation of Mediterranean Fever. 1905. London: Harrison and Sons. Part I, pp. 5–45; part III, pp. 43–55, 71–82.Google Scholar
  85. Robertson, L., Farrell, I. D., Hinchliffe, P. M. 1973. The sensitivity of Brucella abortusto chemotherapeutic agents. Journal of Medical Microbiology 6:549–557.PubMedCrossRefGoogle Scholar
  86. Robertson, L., Farrell, I. D., Hinchliffe, P. M. 1977. The isolation of brucellae from contaminated sources. A review. British Veterinary Journal 133:193–200.PubMedGoogle Scholar
  87. Rode, L. J., Oglesby, G., Schuhardt, V. T. 1950. The cultivation of brucellae on chemically defined media. Journal of Bacteriology 60:661–668.PubMedGoogle Scholar
  88. Sanders, T. H., Higuchi, K., Brewer, C. R. 1953. Studies on the nutrition of Brucella melitensis. Journal of Bacteriology 66:294–299.PubMedGoogle Scholar
  89. Schiemann, B., Staak, C. 1971. Brucella melitensisin impala (Aepyceros melampus). Veterinary Record 88:344.PubMedCrossRefGoogle Scholar
  90. Stoenner, H. G., Lackman, D. B. 1957. A new species of Brucellaisolated from the desert wood rat, Neotoma lepidaThomas. American Journal of Veterinary Research 69:947–951.Google Scholar
  91. Szyfres, B., Tome, J. G. 1966. Natural Brucellainfection in Argentine wild foxes. World Health Organization Bulletin 34:919–923.Google Scholar
  92. Taran, I. F. 1959. The importance of routes of circulation of the’ brucellosis pathogen among saigas. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii 36:110–114.Google Scholar
  93. Thimm, B., Wundt, W. 1976. The epidemiological situation of brucellosis in Africa, pp. 201–217. In: Regamey, R. H., Hulse, E. C., Valette, L. (eds.), International Symposium on Brucellosis (II). Basel: Karger.Google Scholar
  94. Traum, J. 1914. Report of the Chief of the Bureau of Animal Industry, p. 30. Washington, D.C.: United States Department of Agriculture.Google Scholar
  95. Tserendash, Ch., Schumilov, K. V. 1970. Diagnosis of brucellosis of camels. Veterinariya (Moscow) 47:116–117.Google Scholar
  96. United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Diagnostic Reagents Division. 1965. Ames, Iowa, 1965, Manual Nos. 64A: The Propagating of Brucella abortus1119–3 for the Production of brucellaAntigens. 65B: The Production of Brucella abortusStandard Agglutination Test Antigens. 65C: The Production of Brucellosis Supplemental Test Antigens and Reagents. Ames, Iowa: USDA ARS National Animal Disease Center.Google Scholar
  97. Valette, L., Stellman, C., Precausta, P., Desmettre, P., LePemp, M. 1977. Freeze-drying of Brucellavaccine strain B 19, pp. 313–322 In: Cabasso, V. J., Regamey, R. H. (eds.), International Symposium on Freeze-Drying of Biological Products. Basel: Karger.Google Scholar
  98. Verger, J. M., Grayon, M. 1977. Oxidative metabolic profiles of Brucellaspecies. Annali Sclavo 19:54–60.Google Scholar
  99. Verishilova, P. A., Dranovskaya, E. A., Kushmarev, V. M. 1972. An additional method of determination of bacterial reference to the genus Brucella. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii 49:98–101.Google Scholar
  100. Weed, L. A. 1957. Use of a selective medium for isolation of Brucellafrom contaminated surgical specimens. American Journal of Clinical Pathology 27:482–485.PubMedGoogle Scholar
  101. Wilson, G. S., Miles, A. 1975. Topley and Wilson’s principles of bacteriology, virology and immunity, pp. 1054–1078. Baltimore: Williams & Wilkins.Google Scholar
  102. Zabrodin, V. A. 1970. Brucellosis of wild animals in the north polar regions. Veterinariya (Moscow) 47:56–57.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1981

Authors and Affiliations

  • Margaret E. Meyer

There are no affiliations available

Personalised recommendations