Anterior Mediastinal Tumors: A Clinicopathologic Study of 100 Cases, with Emphasis on Immunohistochemical Analysis

  • Mark R. Wick
  • Ross W. Simpson
  • Gloria A. Niehans
  • Bernd W. Scheithauer


PERHAPS BECAUSE THEY ARE UNCOMMON, anterior mediastinal neoplasms continue to present diagnostic challenges to the surgical pathologist. Such tumors have been the subject of many articles and monographs, 1–99 but are still incompletely understood and misdiagnosed. In selected cases, a distinction between the lesions that may arise in the anterior mediastinum is difficult even when they have been resected completely and remain available for detailed study in the laboratory. Current medical practice has added to that difficulty further, however, in that pathologists are being asked to make increasingly more sophisticated interpretations from the ever smaller tissue specimens obtained by needle biopsy. 100–103


Carcinoid Tumor Germ Cell Tumor Human Chorionic Gonadotropin Embryonal Carcinoma Thymic Carcinoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Hodge J, Aponte G, McLaughlin E: Primary mediastinal tumors. J Thorac Cardiovasc Surg 37:730–744, 1959.Google Scholar
  2. 2.
    Daniel RA, Jr, Diveley WL, Edwards WH, et al: Mediastinal tumors. Ann Surg 151:783–795, 1960.PubMedCrossRefGoogle Scholar
  3. 3.
    Friedman NB: Tumors of the thymus. J Thorac Cardiovasc Surg 53:163–182, 1967.PubMedGoogle Scholar
  4. 4.
    Sellors TH, Thackray AC, Thomson AD: Tumours of the thymus: A review of 88 operation cases. Thorax 22:193–220, 1967.PubMedCrossRefGoogle Scholar
  5. 5.
    Lattes R: Thymoma and other tumors of the thymus: An analysis of 107 cases. Cancer 15:1224–1260, 1962.CrossRefGoogle Scholar
  6. 6.
    Bergh NP, Gatzinsky P, Larsson S, et al: Tumors of the thymus and thymic region: I. Clinicopathological studies on thymomas. Ann Thorac Surg 25:91–98, 1978.PubMedCrossRefGoogle Scholar
  7. 7.
    Gray GF, Gutowski WT: Thymoma: A clinico—pathologic study of 54 cases. Am J Surg Pathol 3:235–249, 1979.PubMedCrossRefGoogle Scholar
  8. 8.
    Bernatz PE, Harrison EG, Jr, Clagett OT: Thymoma: A clinicopathologic study. J Thorac Car—diovasc Surg 42:424–444, 1961.Google Scholar
  9. 9.
    Legg MA, Brady WJ: Pathology and clinical behavior of thymomas: a survey of 51 cases. Cancer 18:1131–1144, 1965.PubMedCrossRefGoogle Scholar
  10. 10.
    Bernatz PE, Khonsari S, Harrison EG, Jr, et al: Thymoma: factors influencing prognosis. Surg Clin North Amer 53:885–892, 1973.Google Scholar
  11. 11.
    Weissberg D, Goldberg M, Pearson FG: Thymoma. Ann Thorac Surg 16:141–147, 1973.CrossRefGoogle Scholar
  12. 12.
    Batata MA, Martini N, Huvos AG, et al: Thymomas: Clinicopathologic features, therapy, and prognosis. Cancer 34:389–396, 1974.PubMedCrossRefGoogle Scholar
  13. 13.
    Jain U, Frable WJ: Thymoma: Analysis of benign and malignant criteria. J Thorac Cardiovasc Surg 67:310–321, 1974.PubMedGoogle Scholar
  14. 14.
    Salyer WR, Eggleston JC: Thymoma: A clinical and pathological study of 65 cases. Cancer 37:229–249, 1976.PubMedCrossRefGoogle Scholar
  15. 15.
    LeGolvan DP, Abell MR: Thymomas. Cancer 39:2142–2157, 1977.PubMedCrossRefGoogle Scholar
  16. 16.
    Gerein AN, Srivastava SP, Burgess J: Thymoma: A ten year review. Am J Surg 136:49–52, 1978.PubMedCrossRefGoogle Scholar
  17. 17.
    Otto HF, Husselman H: A comparative clinical and pathological study on the classification and prognostic features of 57 thymomas. I. Micros—copy and ultrastructural pathology. Z Krebforsch 91:81–102, 1978.CrossRefGoogle Scholar
  18. 18.
    Wilkins EW Jr, Castleman B: Thymoma: a continuing survey at the Massachusetts General Hos—pital. Ann Thorac Surg 28:252–255, 1979.PubMedCrossRefGoogle Scholar
  19. 19.
    Verley JM, Hollmann KH: Thymoma: A comparative study of clinical stages, histologic features, and survival in 200 cases. Cancer 55:1074–1086, 1985.PubMedCrossRefGoogle Scholar
  20. 20.
    Masaoka A, Monden Y, Nakahara K, et al: Followup study of thymomas with special refer—ence to their clinical stages. Cancer 48:2485–2492, 1981.PubMedCrossRefGoogle Scholar
  21. 21.
    Maggi G, Giaccone G, Donadio M, et al: Thymomas: A review of 169 cases with particular reference to results of surgical treatment. Cancer 58:765–776, 1986.PubMedCrossRefGoogle Scholar
  22. 22.
    Muller-Hermelink HK, Marino M, Palestro G: Pathology of thymic epithelial tumors. Curr Topics Pathol 75:207–268, 1986.CrossRefGoogle Scholar
  23. 23.
    Osborne B, Mackay B, Battifora H: Thymoma: A clinicopathologic study of 23 cases. Pathol Annu 20(2):289–315, 1985.PubMedGoogle Scholar
  24. 24.
    Marino M, Muller-Hermelink HK: Thymoma and thymic carcinoma: Relation of thymoma epi—thelial cells to the cortical and medullary differen—tiation of thymus. Virchows Arch Pathol Anat 407:119–149, 1985.CrossRefGoogle Scholar
  25. 25.
    Baud M, Stamenkovic I, Kapanci Y: Malignant thymomas: Clinicopathologic study of 13 cases. Prog Surg Pathol 3:129–146, 1981.Google Scholar
  26. 26.
    Levine GD, Rosai J, Bearman RM, et al: The fine structure of thymoma, with emphasis on its differential diagnosis: A study of ten cases. Am J Pathol 81:49–86, 1975.PubMedGoogle Scholar
  27. 27.
    Sheir KJ: The thymus according to Schambacher: medullary ducts and reticular epithelium of thymus and thymomas. Cancer 48:1183–1199, 1981.CrossRefGoogle Scholar
  28. 28.
    Swinborne-Sheldrake K, Gray GF, Jr, Glick AD: Thymic epithelial neoplasms. South Med J 78:790–800, 1985.PubMedCrossRefGoogle Scholar
  29. 29.
    Ring NP, Addis BJ: Thymoma: An integrated clinicopathological and immunohistochemical study. J Pathol 149:327–337, 1986.PubMedCrossRefGoogle Scholar
  30. 30.
    Lewis JE, Wick MR, Scheithauer BW, et al: Thymoma: A clinicopathologic review. Cancer 60:2727–2743, 1987.PubMedCrossRefGoogle Scholar
  31. 31.
    Kornstein MJ, Curran WJ, Jr, Turrisi AT III, et al: Cortical versus medullary thymomas: A useful morphologic distinction? Hum Pathol 19:1335–1339, 1988.PubMedCrossRefGoogle Scholar
  32. 32.
    Snover DC, Levine GD, Rosai J: Thymic carcinoma: Five distinctive histologic variants. Am J Surg Pathol 6:451–470, 1982.PubMedCrossRefGoogle Scholar
  33. 33.
    Wick MR, Scheithauer BW, Weiland LH, et al: Primary thymic carcinomas. Am J Surg Pathol 6:613–630, 1982.PubMedCrossRefGoogle Scholar
  34. 34.
    Alguacil-Garcia A, Halliday WC: Thymic carci—noma with focal neuroblastoma differentiation. Am J Surg Pathol 11:474–479, 1987.PubMedCrossRefGoogle Scholar
  35. 35.
    Ringertz N, Lidholm SO: Mediastinal tumors and cysts. J Thorac Surg 31:458–487, 1956.PubMedGoogle Scholar
  36. 36.
    Rosenow EC III, Hurley BT: Disorders of the thymus: A review. Arch Intern Med 144:763–770, 1984.PubMedCrossRefGoogle Scholar
  37. 37.
    Levine GD, Rosai J: Thymic hyperplasia and neoplasia: A review of current concepts. Hum Pathol 9:495–515, 1978.PubMedCrossRefGoogle Scholar
  38. 38.
    Rosai J, Levine GD: Tumors of the thymus, In Atlas of Tumor Pathology, Second Series, Fascicle 13, Armed Forces Institute of Pathology, Washington, D.C., 1976.Google Scholar
  39. 39.
    Rosai J, Higa E; Mediastinal endocrine neoplasm of probable thymic origin, related to carcinoid tumor: Clinicopathologic study of 8 cases. Cancer 29:1061–1074, 1972.PubMedCrossRefGoogle Scholar
  40. 40.
    Rosai J, Levine GD, Weber WR, et al: Carcinoid tumors and oat cell carcinomas of the thymus. Pathol Annu 11:201–226, 1976.PubMedGoogle Scholar
  41. 41.
    Levine GD, Rosai J: A spindle cell variant of thymic carcinoid tumor. Arch Pathol Lab Med 100:293–300, 1976.PubMedGoogle Scholar
  42. 42.
    Salyer WR, Salyer DC, Eggleston JC: Carcinoid tumors of the thymus. Cancer 37:958–973, 1976.PubMedCrossRefGoogle Scholar
  43. 43.
    Otto HF, Husselmann H, Gebbers JO: Thymic carcinoid tumors. Electron microscopic and histochemical observations. Verh Deutsch Ges Pathol 61:122–126, 1977.Google Scholar
  44. 44.
    Lokich JJ, Li F: Carcinoid tumor of the thymus with hereditary hyperparathyroidism. Ann Intern Med 89:364–365, 1978.PubMedCrossRefGoogle Scholar
  45. 45.
    Wick MR, Scott RE, Li CY, et al: Carcinoid tumor of the thymus: A clinicopathologic report of seven cases with a review of the literature. Mayo Clin Proc 55:246–254, 1980.PubMedGoogle Scholar
  46. 46.
    Lieske TR, Kincaid J, Sunderrajan EV: Thymic carcinoid with cutaneous hyperpigmentation. Arch Intern Med 145:361–363, 1985.PubMedCrossRefGoogle Scholar
  47. 47.
    LaGrange W, Dahm HH, Karstens J, et al: Me—lanocytic neuroendocrine carcinoma of the thymus. Cancer 59:484–488, 1987.PubMedCrossRefGoogle Scholar
  48. 48.
    Herbst WM, Kummer W, Hofmann W, et al: C: Carcinoid tumors of the thymus: An immunohis—tochemical study. Cancer 60:2465–2470, 1987.PubMedCrossRefGoogle Scholar
  49. 49.
    Viebahn R, Hiddemann W, Klinke F, et al: Thymus carcinoid. Pathol Res Pract 180:445–448, 1985.PubMedCrossRefGoogle Scholar
  50. 50.
    Hunkatroon M, Lin F, Heitz PU, et al: Thymic carcinoid tumor with Cushing’s syndrome. Arch Pathol Lab Med 108:551–554, 1984.Google Scholar
  51. 51.
    Wick MR, Carney JA, Bernatz PE, et al: Primary mediastinal carcinoid tumors. Am J Surg Pathol 6:195–205, 1982.PubMedCrossRefGoogle Scholar
  52. 52.
    Lowenthal RM, Gumpel JM, Kreel L, et al: Carcinoid tumor of the thymus with systemic mani—festations: A radiological and pathological study. Thorax 29:553–558, 1974.PubMedCrossRefGoogle Scholar
  53. 53.
    Manes JL, Taylor HB: Thymic carcinoid in fa—miliar multiple endocrine adenomatosis. Arch Pathol Lab Med 95:252–258, 1973.Google Scholar
  54. 54.
    Marchevsky AM, Dickman SH: Mediastinal carcinoid with an incomplete Sipple’s syndrome. Cancer 43:2497–2501, 1979.PubMedCrossRefGoogle Scholar
  55. 55.
    Wick MR, Scheithauer BW: Thymic carcinoid: A histologic, immunohistochemical, and ultrastructural study of 12 cases. Cancer 53:475–484, 1984.PubMedCrossRefGoogle Scholar
  56. 56.
    Wick MR, Bahn RC, Scholz DA: Retrospective ultrastructural study of three thymic neoplasms associated with Cushing’s syndrome: Evidence of their neuroendocrine nature. Minn Med 66:114–117, 1983.PubMedGoogle Scholar
  57. 57.
    Wick MR, Scheithauer BW: Oat—cell carcinoma of the thymus. Cancer 49:1652–1657, 1982.PubMedCrossRefGoogle Scholar
  58. 58.
    Pugsley WS, Carleton RL: Germinal nature of teratoid tumors of the thymus. Arch Pathol 56:341–347, 1953.Google Scholar
  59. 59.
    Adebonojo SA, Nicola ML: Teratoid tumors of the mediastinum. Am Surg 42:361–365, 1976.PubMedGoogle Scholar
  60. 60.
    Luna MA, Valenzuela—Tamril J: Germ cell tumors of the mediastinum: Postmortem find—ings. Am J Clin Pathol 65:450–454, 1976.PubMedGoogle Scholar
  61. 61.
    Aygun C, Slawson RG, Bajaj K, et al: Primary mediastinal seminoma. Urology 23:109–117, 1984.PubMedCrossRefGoogle Scholar
  62. 62.
    Burt ME, Javadpour N: Germ cell tumors in patients with apparently normal testes. Cancer 47:1911–1915, 1981.PubMedCrossRefGoogle Scholar
  63. 63.
    Canty TG, Siemens R: Malignant mediastinal teratoma in a 15 year old girl. Cancer 41:1623–1626, 1978.PubMedCrossRefGoogle Scholar
  64. 64.
    Carter D, Bibro MC, Touloukian RJ: Benign clinical behavior of immature mediastinal teratoma in infancy and childhood: Report of two cases and review of the literature. Cancer 49:398–402, 1982.PubMedCrossRefGoogle Scholar
  65. 65.
    Cox JD: Primary malignant germinal tumors of the mediastinum: A study of 24 cases. Cancer 36:1162–1168, 1975.PubMedCrossRefGoogle Scholar
  66. 66.
    DeSmet AA, Silver TM, Hart WR: Endodermal sinus tumor of the anterior mediastinum. South Med J 70:757–758, 1977.PubMedCrossRefGoogle Scholar
  67. 67.
    Fox MA, Vix VA: Endodermal sinus (yolk sac) tumor of the anterior mediastinum. Am J Roent—genol 135:291–294, 1980.CrossRefGoogle Scholar
  68. 68.
    Friedman NB: The comparative morphogenesis of extragenital and gonadal teratoid tumors. Cancer 4:265–276, 1951.PubMedCrossRefGoogle Scholar
  69. 69.
    Schantz A, Sewall W, Castleman B: Mediastinal germinoma: A study of 21 cases with an excellent prognosis. Cancer 30:1189–1194, 1972.PubMedCrossRefGoogle Scholar
  70. 70.
    Oberman HA, Libcke JH: Malignant germinal neoplasms of the mediastinum. Cancer 17:498–597, 1964.PubMedCrossRefGoogle Scholar
  71. 71.
    Huntington RW Jr, Bullock WK: Yolk sac tumors of extragonadal origin. Cancer 25:1358–1366, 1970.Google Scholar
  72. 72.
    Wick MR, Siegal GP: Primary mediastinal embryonal carcinoma. Minn Med 63:723–726, 1980.PubMedGoogle Scholar
  73. 73.
    Knapp RH, Fritz SR, Reiman H: Primary embryonal carcinoma and choriocarcinoma of the mediastinum. Arch Pathol Lab Med 106:507–509, 1982.PubMedGoogle Scholar
  74. 74.
    Knapp RH, Hurt RD, Payne S, et al: Malignant germ cell tumors of the mediastinum. J Thorac Cardiovasc Surg 89:82–89, 1985.PubMedGoogle Scholar
  75. 75.
    Levine GD: Primary thymic seminoma—a neoplasm ultrastructurally similar to testicular seminoma and distinct from epithelial thymoma. Cancer 31:729–741, 1973.PubMedCrossRefGoogle Scholar
  76. 76.
    Wenger ME, Dines DE, Ahmann DL, et al: Primary mediastinal choriocarcinoma. Mayo Clin Proc 43:570–575, 1968.PubMedGoogle Scholar
  77. 77.
    Recondo J, Libshitz HI: Mediastinal extragonadal germ cell tumors. Urology 11:369–375, 1978.PubMedCrossRefGoogle Scholar
  78. 78.
    Lewis BD, Hurt RD, Payne WS, et al: Benign teratomas of the mediastinum. J Thorac Cardiovasc Surg 86:727–731, 1983.PubMedGoogle Scholar
  79. 79.
    Manivel JC, Wick MR, Abenoza P, et al: The occurrence of sarcomatous components in pri—mary mediastinal germ cell tumors. Am J Surg Pathol 10:711–717, 1986.PubMedCrossRefGoogle Scholar
  80. 80.
    Burns BF, McCaughey WTE: Unusual thymic seminomas. Arch Pathol Lab Med 110:539–541, 1986.PubMedGoogle Scholar
  81. 81.
    Marshall AHE, Wood C: The involvement of the thymus in Hodgkin’s disease. J Pathol Bacteriol 73:163–166, 1957.CrossRefGoogle Scholar
  82. 82.
    Martini N, Golbey RB, Hajdu SI, et al: Primary mediastinal germ cell tumors. Cancer 33:763–769, 1974.PubMedCrossRefGoogle Scholar
  83. 83.
    Harms D, Janig U: Germ cell tumors of childhood: Report of 170 cases including 59 pure and partial yolk—sac tumors. Virchows Arch Pathol Anat 409:223–239, 1986.CrossRefGoogle Scholar
  84. 84.
    Mauch P, Hellman S: Mediastinal Hodgkin’s disease: Significance of mediastinal involvement in early stage Hodgkin’s disease. Hematol Oncol 2:69–72, 1984.PubMedCrossRefGoogle Scholar
  85. 85.
    Keller AR, Castleman B: Hodgkin’s disease of the thymus gland. Cancer 33:1615–1623, 1974.PubMedCrossRefGoogle Scholar
  86. 86.
    Katz A, Lattes R: Granulomatous thymoma or Hodgkin’s disease of the thymus? A clinical and histologic study and a re—evaluation. Cancer 23:1–15, 1969.PubMedCrossRefGoogle Scholar
  87. 87.
    Fechner RE: Hodgkin’s disease of the thymus. Cancer 23:16–23, 1969.PubMedCrossRefGoogle Scholar
  88. 88.
    Miller JB, Variakojis D, Bitran JD, et al: Diffuse histiocytic lymphoma with sclerosis: A clinico—pathologic entity frequently causing superior vena caval obstruction. Cancer 47:748–756, 1981.PubMedCrossRefGoogle Scholar
  89. 89.
    Smith JL, Barker CR, Klein GP, et al: Characteri—sation of malignant mediastinal lymphoid neo—plasm (Sternberg’s sarcoma) as thymic in origin. Lancet 1:74–77, 1973.PubMedCrossRefGoogle Scholar
  90. 90.
    Nathwani BN, Kim H, Rappaport H: Malignant lymphoma, lymphoblastic. Cancer 38:964–983, 1976.PubMedCrossRefGoogle Scholar
  91. 91.
    Lichtenstein AK, Levine A, Taylor R, et al: Primary mediastinal lymphoma in adults. Am J Med 68:509–514, 1980.PubMedCrossRefGoogle Scholar
  92. 92.
    Levitt LJ, Aisenberg AC, Harris NL, et al: Primary non—Hodgkin’s lymphoma of the mediasti—num. Cancer 50:2486–2492, 1982.PubMedCrossRefGoogle Scholar
  93. 93.
    Trump DL, Mann RB: Diffuse large cell and undifferentiated lymphomas with prominent me—\diastinal involvement: A poor prognostic subset of patients with non—Hodgkin’s lymphoma. Cancer 50:277–282, 1982.PubMedCrossRefGoogle Scholar
  94. 94.
    Perrone T, Frizzera G, Rosai J: Mediastinal diffuse large—cell lymphoma with sclerosis: A clinicopathologic study of 60 cases. Am J Surg Pathol 10:176–191, 1986.PubMedCrossRefGoogle Scholar
  95. 95.
    Olson JL, Salyer WR: Mediastinal paraganglio—mas (aortic body tumors:) a report of four cases and a review of the literature. Cancer 41:2405–2412, 1978.PubMedCrossRefGoogle Scholar
  96. 96.
    Pachter MR, Lattes R: Mesenchymal tumors of the mediastinum. I. Tumors of fibrous tissue, adipose tissue, smooth muscle, and striated muscle. Cancer 16:74–94, 1963.PubMedCrossRefGoogle Scholar
  97. 97.
    Razzuk MA, Urschel HC Jr, Race GJ, et al: Liposarcoma of the mediastinum: Case report and review of the literature. J Thorac Cardiovasc Surg 61:819–826, 1971.PubMedGoogle Scholar
  98. 98.
    Uner R, Balim AI, Oketem K: Mediastinal liposarcoma: Report of a case. Dis Chest 43:103–105, 1963.PubMedCrossRefGoogle Scholar
  99. 99.
    Russell CF, Edis AJ, Scholz DA, et al: Mediastinal parathyroid tumors: Experience with 38 tumors requiring mediastinotomy for removal. Ann Surg 193:805–809, 1981.PubMedCrossRefGoogle Scholar
  100. 100.
    Jereb M, Us—Krasovec M: Transthoracic needle biopsy of mediastinal and hilar lesions. Cancer 40:1354–1357, 1977.PubMedCrossRefGoogle Scholar
  101. 101.
    Haaga JR: New techniques in CT—guided biop—sies. Am J Roentgenol 133:633–641, 1979.CrossRefGoogle Scholar
  102. 102.
    Rosenberger A, Adler O: Fine needle aspiration in the diagnosis of mediastinal lesions. Am J Roentgenol 131:239–242, 1978.CrossRefGoogle Scholar
  103. 103.
    Sterrett G, Whitaker D, Shilkin KB, et al: The fine needle aspiration cytology of mediastinal lesions. Cancer 51:127–135, 1983.PubMedCrossRefGoogle Scholar
  104. 104.
    Battifora H, Sun TT, Bahu RM, et al: The use of anti—keratin antiserum as a diagnostic tool: Thymoma versus lymphoma. Hum Pathol 11:635–641, 1980.PubMedCrossRefGoogle Scholar
  105. 105.
    Kodama T, Watanabe S, Sato Y, et al: An immunohistochemical study of thymic epithelial tumors. I. Epithelial component. Am J Surg Pathol 10:26–33, 1986.PubMedCrossRefGoogle Scholar
  106. 106.
    Sato Y, Watanabe S, Mukai K, et al: An immunohistochemical study of thymic epithelial tumors. II. Lymphoid component. Am J Surg Pathol 10:862–870, 1986.PubMedCrossRefGoogle Scholar
  107. 107.
    Chan WC, Zaatari GS, Tabei S, et al: Thymoma: An immunohistochemical study. Am J Clin Pathol 82:160–166, 1984.PubMedGoogle Scholar
  108. 108.
    Mokhtar N, Hsu SM, Lad RP, et al: Thymoma: Lymphoid and epithelial components mirror the phenotype of the normal thymus. Hum Pathol 15:378–384, 1984.PubMedCrossRefGoogle Scholar
  109. 109.
    Kornstein MJ, Hoxie JA, Levinson AI, et al: Immunohistology of human thymomas. Arch Pathol Lab Med 109:460–463, 1985.PubMedGoogle Scholar
  110. 110.
    Van der Kwast TH, Van Vliet E, Cristen E, et al: An immunohistologic study of the epithelial and lymphoid components of six thymomas. Hum Pathol 16:1001–1008, 1985.PubMedCrossRefGoogle Scholar
  111. 111.
    Hofmann WJ, Momburg F, Moller P: Thymic medullary cells expressing B lymphocyte antigens. Hum Pathol 19:1280–1287, 1988.PubMedCrossRefGoogle Scholar
  112. 112.
    Savino W, Durand D, Dardenne M: Immunohistochemical evidence for the expression of the carcinoembryonic antigen by human thymic epithelial cells in vitro and in neoplastic conditions. Am J Pathol 121:418–425, 1985.PubMedGoogle Scholar
  113. 113.
    Altmannsberger M, Osborn M, Schauer A, et al: Antibodies to different intermediate filament proteins: Cell type—specific markers on paraffin—embedded human tissues. Lab Invest 45:427–434, 1981.PubMedGoogle Scholar
  114. 114.
    Battifora H, Sheibani K, Tubbs RR, et al: Anti—keratin antibodies in tumor diagnosis: Distinction between seminoma and embryonal carcinoma. Cancer 54:843–848, 1984.PubMedCrossRefGoogle Scholar
  115. 115.
    Nagle RB, McDaniel KM, Clark VA, et al: The use of antikeratin antibodies in the diagnosis of human neoplasms. Am J Clin Pathol 79:458–466, 1983.PubMedGoogle Scholar
  116. 116.
    Battifora H: Diagnostic uses of antibodies to keratins. in Feroglio, CM, Wolff, M and Rilke, F. (Eds) in Progress in Surgical Pathology, Philadelphia, Field & Woods, Inc. 8:1–15, 1988.CrossRefGoogle Scholar
  117. 117.
    Miettinen M, Partanen S, Lehto VP, et al: Mediastinal tumors: Ultrastructural and immunohistochemical evaluation of intermediate filaments as diagnostic aids. Ultrastructural Pathol 4:337–347, 1983.CrossRefGoogle Scholar
  118. 118.
    Gatter KC, Alcock C, Heryet A, et al: DY: The differential diagnosis of routinely processed anaplastic tumors using monoclonal antibodies. Am J Clin Pathol 82:33–43, 1984.PubMedGoogle Scholar
  119. 119.
    Osborn M, Altmannsberger M, Debus E, et al: Differentiation of the major human tumor groups using conventional and monoclonal antibodies specific for individual intermediate filament proteins. Ann NY Acad Sci 455:649–668, 1985.PubMedCrossRefGoogle Scholar
  120. 120.
    Poston RN, Sidhu YS: Diagnosing tumours on routine surgical sections by immunohistochemistry. J Clin Pathol 39:514–523, 1986.PubMedCrossRefGoogle Scholar
  121. 121.
    Kahn HJ, Thorner PS, Yeger H, et al: Distinct keratin patterns demonstrated by immunoperoxidase staining of adenocarcinomas, carcinoids, and mesotheliomas using polyclonal and monoclonal anti—keratin antibodies. Am J Clin Pathol 86:566–574, 1986.PubMedGoogle Scholar
  122. 122.
    Pinkus GS, Kurtin PJ: Epithelial membrane antigen —a diagnostic discriminant in surgical pathology. Hum Pathol 16:929–940, 1985.PubMedCrossRefGoogle Scholar
  123. 123.
    Sloane JP, Ormerod MG: Distribution of epithelial membrane antigen in normal and neoplastic tissues and its value in diagnostic tumor pathology. Cancer 47:1786–1795, 1981.PubMedCrossRefGoogle Scholar
  124. 124.
    Kurtin PJ, Pinkus GS: Leukocyte common antigen— a diagnostic discriminant between hematopoietic and nonhematopoietic neoplasms in paraffin sections using monoclonal antibodies. Hum Pathol 16:353–365, 1985.PubMedCrossRefGoogle Scholar
  125. 125.
    Michels S, Swanson PE, Frizzera G, et al: Immunostaining for leukocyte common antigen using an amplified avidin—biotin—peroxidase complex method and paraffin sections. Arch Pathol Lab Med 111:1035–1039, 1987.PubMedGoogle Scholar
  126. 126.
    Knowles DM II: Lymphoid cell markers: Their distribution and usefulness in the immunophenotypic analysis of lymphoid neoplasms. Am J Surg Pathol 9:85–108, 1985.Google Scholar
  127. 127.
    Van der Valk P, Van den Besselaar—Dingjan G, Daha MR, et al: Analysis of large—cell lymphomas using monoclonal and heterologous antibodies. J Clin Pathol 36:44–50, 1983.PubMedCrossRefGoogle Scholar
  128. 128.
    Hsu SM, Jaffe ES: Leu M1 and peanut agglutinin stain the neoplastic cells of Hodgkin’s disease. Am J Clin Pathol 82:29–32, 1984.PubMedGoogle Scholar
  129. 129.
    Hsu SM, Yang K, Jaffe ES: Phenotypic expression of Hodgkin’s and Reed—Sternberg cells in Hodgkin’s disease. Am J Pathol 118:209–217, 1985.PubMedGoogle Scholar
  130. 130.
    Pinkus GS, Thomas P, Said JW: Leu M1 — a marker for Reed Sternberg cells in Hodgkin’s disease: An immunoperoxidase study of paraffinembedded tissues. Am J Pathol 119:244–252, 1985.PubMedGoogle Scholar
  131. 131.
    Frierson HF Jr, Innes DJ: Sensitivity of anti—Leu M1 as a marker in Hodgkin’s disease. Arch Pathol Lab Med 109:1024–1028, 1985.PubMedGoogle Scholar
  132. 132.
    Sheibani K, Battifora H, Burke JS, et al: Leu M1 antigen in human neoplasms: An immunohistologic study of 400 cases. Am J Surg Pathol 10:227–236, 1986.PubMedCrossRefGoogle Scholar
  133. 133.
    Ramaekers FCS, Feitz W, Moesker O, et al: Antibodies to cytokeratin and vimentin in testicular tumor diagnosis. Virchows Arch Pathol Anat 408:127–142, 1985.CrossRefGoogle Scholar
  134. 134.
    Denk H, Moll R, Weybora W, et al: Intermediate filaments and desmosomal plaque proteins in testicular seminomas and non—seminomatous germ cell tumors as revealed by immunohistochemistry. Virchows Arch Pathol Anat 410:295–307, 1987.CrossRefGoogle Scholar
  135. 135.
    Miettinen M, Virtanen I, Talerman A: Intermediate filament proteins in human testis and testicular germ cell tumors. Am J Pathol 120:402–410, 1985.PubMedGoogle Scholar
  136. 136.
    Jacobsen GK, Jacobsen M, Clausen PP: Distribution of tumor—associated antigens in the various histologic components of germ cell tumors of the testis. Am J Surg Pathol 5:257–265, 1981.PubMedCrossRefGoogle Scholar
  137. 137.
    Jacobsen GK, Jacobsen M: Alpha—fetoprotein (AFP) and human chorionic gonadotropin (HCG) in testicular germ cell tumors: A prospective immunohistochemical study. Acta Pathol Microbiol Immunol Scand (A) 91:165–176, 1983.Google Scholar
  138. 138.
    Paiva J, Damjanov I, Lange PH, et al: Immunohistochemical localization of placental—like alkaline phosphatase in testis and germ—cell tumors using monoclonal antibodies. Am J Pathol 111:156–165, 1983.PubMedGoogle Scholar
  139. 139.
    Wick MR, Swanson PE, Manivel JC: Placental—like alkaline phosphatase reactivity in human tumors: An immunohistochemical study of 520 cases. Hum Pathol 18:946–954, 1987.PubMedCrossRefGoogle Scholar
  140. 140.
    Maiorana A, Sannicola C, Trentini GP, et al: Antigenic markers in extragonadal germ cell tumors. Appl Pathol 2:96–102, 1984.PubMedGoogle Scholar
  141. 141.
    Niehans GA, Manivel JC, Copland GT, et al: Immunohistochemistry of germ cell and trophoblastic neoplasms. Cancer 62:1113–1123, 1988.PubMedCrossRefGoogle Scholar
  142. 142.
    Sandhaus L, Strom RL, Mukai K: Primary embryonal—choriocarcinoma of the mediastinum in a woman: A case report with immunohistochemical study. Am J Clin Pathol 75:573–578, 1981.PubMedGoogle Scholar
  143. 143.
    Sass M, Jao W, Horn T, et al: Mediastinal Yolk sac tumor: Ultrastructural and immunofluorescent studies. Ultrastructural Pathol 4:67–73, 1983.CrossRefGoogle Scholar
  144. 144.
    Tapia FJ, Polak JM, Barbosa AJA, et al: Neuron—specific enolase is produced by neuroendocrine tumors. Lancet 1:808–811, 1981.PubMedCrossRefGoogle Scholar
  145. 145.
    Wick MR, Scheithauer BW, Kovacs K: Neuronspecific enolase in neuroendocrine tumors of the thymus, bronchus, and skin. Am J Clin Pathol 79:703–707, 1983.PubMedGoogle Scholar
  146. 146.
    Tsutsumi Y: Leu 7 immunoreactivity as a histochemical marker for paraffin—embedded neuroendocrine tumors. Acta Histochem Cytochem 17:15–21, 1984.CrossRefGoogle Scholar
  147. 147.
    Perentes E, Rubinstein LJ: Immunohistochemi—cal recognition of human neuroepithelial tumors by anti—Leu 7 (HNK—1) monoclonal antibody. Acta Neuropathol 69:227–233, 1986.PubMedCrossRefGoogle Scholar
  148. 148.
    Bunn PA, Linnoila I, Minna JD, et al: Small cell lung cancer, endocrine cells of the fetal bronchus, and other neuroendocrine cells express the Leu 7 antigenic determinant present on natural killer cells. Blood 65:764–768, 1985.PubMedGoogle Scholar
  149. 149.
    Michels S, Swanson PE, Robb JA, et al: Leu 7 in small—cell neoplasms. An immunohistochemical study, with ultrastructural correlations. Cancer 60:2958–2964, 1987.PubMedCrossRefGoogle Scholar
  150. 150.
    Wilson BS, Lloyd RV: Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am J Pathol 115:458–468, 1984.PubMedGoogle Scholar
  151. 151.
    Weiler R, Rischer-Colbrie R, Schmid KW, et al: Immunological studies on the occurrence and properties of chromogranin A and B and secretogranin II in endocrine tumors. Am J Surg Pathol 12:877–894, 1988.PubMedCrossRefGoogle Scholar
  152. 152.
    O’Connor DT: Chromogranin: Widespread immunoreactivity in polypeptide hormone producing tissues and in serum. Regulatory Peptides 6:263–280, 1983.PubMedCrossRefGoogle Scholar
  153. 153.
    Hsu SM, Raine L, Fanger H: Use of avidin—biotin—peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580, 1981.PubMedCrossRefGoogle Scholar
  154. 154.
    Colby TV, Hoppe RT, Warnke RA: Hodgkin’s disease: A clinicopathologic study of 659 cases. Cancer 49:1848–1858, 1982.PubMedCrossRefGoogle Scholar
  155. 155.
    Vogelzang NJ, Raghavan D, Anderson RW, et al: Mediastinal non—seminomatous germ cell tumors: The role of combined modality therapy. Ann Thorac Surg 33:333–339, 1982.PubMedCrossRefGoogle Scholar
  156. 156.
    Hutchison GB: Radiotherapy of Stage I and II Hodgkin’s disease: a collaborative study. Cancer 54:1928–1942, 1984.CrossRefGoogle Scholar
  157. 157.
    Azumi N, Battifora H: The distribution of vimentin and keratin in epithelial and non—epithelial neoplasms. Am J Clin Pathol 88:286–296, 1987.PubMedGoogle Scholar
  158. 158.
    Thomas P, Battifora H: Keratins versus epithelial membrane antigen in tumor diagnosis: An immunohistochemical comparison of five monoclonal antibodies. Hum Pathol 18:728–734, 1987.PubMedCrossRefGoogle Scholar
  159. 159.
    Wick MR, Swanson PE, Manivel JC: Immunohistochemical diagnosis of soft tissue sarcomas: Comparisons with electron microscopy. Applied Pathol 6:169–196, 1988.Google Scholar
  160. 160.
    Dorfman RF, Gatter KC, Pulford KAF, et al: An evaluation of the utility of anti—granulocyte and anti—leukocyte monoclonal antibodies in the diagnosis of Hodgkin’s disease. Am J Pathol 123:508–519, 1986.PubMedGoogle Scholar
  161. 161.
    Swanson PE: Monoclonal antibodies to monomorphic human histocompatibility antigens: class I (HLA—A,B,C); class II (HLA—DR or Ia—like antigen), and beta—2—microglobulin, in Wick MR, Siegal GP, (eds): New York, Monoclonal Antibodies in Diagnostic Immunohistochemistry Marcel Dekker, 309–349, 1988.Google Scholar
  162. 162.
    Souadjian JV, Enriquez P, Silverstein MN, et al: The spectrum of diseases associated with thymoma. Arch Intern Med 134:374–379, 1974.PubMedCrossRefGoogle Scholar
  163. 163.
    Addis BJ, Isaacson PG: Large cell lymphoma of the mediastinum: a B—cell tumor of probable thy—mic origin. Histopathology 10:379–390, 1986.PubMedCrossRefGoogle Scholar
  164. 164.
    Andrade RE, Wick MR, Frizzera G, et al: Immunophenotyping of hematopoietic malignancies in paraffin sections. Hum Pathol 19:394–403, 1988.PubMedCrossRefGoogle Scholar
  165. 165.
    Wilson TS, McDowell EM, McIntire KR, et al: Elaboration of human chorionic gonadotropin by lung tumors. Arch Pathol Lab Med 105:169–173, 1981.PubMedGoogle Scholar
  166. 166.
    Wachner R, Wittekind C, Von Kleist S: Immunohistological localization of beta—HCG in breast carcinomas. Eur J Cancer Clin Oncol 20:677–684, 1984.CrossRefGoogle Scholar
  167. 167.
    Yoshimoto T, Higashino K, Hada T, et al: A primary lung carcinoma producing alpha—fetoprotein, carcinoembryonic antigen, and human chorionic gonadotropin. Cancer 60:2744–2750, 1987.PubMedCrossRefGoogle Scholar
  168. 168.
    Wachner R, Wittekind C, Von Kleist S: Localization of carcinoembryonic antigen, beta—HCG, SP 1, and keratin in the tissue of lung carcinomas: An immunohistochemical study. Virchows Arch Pathol Anat 402:415–423, 1984.CrossRefGoogle Scholar
  169. 169.
    Manivel JC, Jessurun J, Wick MR, et al: Placental alkaline phosphatase immunoreactivity in tes—ticular germ cell neoplasms. Am J Surg Pathol 11:21–29, 1987.PubMedCrossRefGoogle Scholar
  170. 170.
    Burke AP, Mostofi FK: Placental alkaline phosphatase immunohistochemistry of intratubular malignant germ cells and associated testicular germ cell tumors. Hum Pathol 19:663–670, 1988.PubMedCrossRefGoogle Scholar
  171. 171.
    Wick MR: Monoclonal antibodies to carcinoem—bryonic antigen in diagnostic immunohistochemistry, in Wick MR, Siegal GP, (eds): Monoclonal Antibodies in Diagnostic Immunohistochemistry New York, Marcel Dekker, 539–567, 1988.Google Scholar
  172. 172.
    Leader M, Collins M, Patel J, et al: Antineuron specific enolase reactions in sarcomas and carcinomas: Its lack of neuroendocrine specificity. J Clin Pathol 39:1186–1197, 1986.PubMedCrossRefGoogle Scholar
  173. 173.
    Haimoto H, Takahashi Y, Koshikawa T, et al: Immunohistochemical localization of gamma—enolase in normal human tissues other than nervous and neuroendocrine tissues. Lab Invest 52:257–263, 1985.PubMedGoogle Scholar
  174. 174.
    Tischler AS, Mobtaker H, Mann K, et al: Antilymphocyte antibody Leu—7 (HNK—1) recognizes a constituent of neuroendocrine granule matrix. J Histochem Cytochem 34:1213–1216, 1986.PubMedCrossRefGoogle Scholar
  175. 175.
    Swanson PE, Manivel JC, Wick MR: Immunor—eactivity for Leu 7 in neurofibrosarcomas and other spindle cell sarcomas of soft tissue. Am J Pathol 126:546–560, 1987.PubMedGoogle Scholar
  176. 176.
    Rusthoven JJ, Robinson JB, Kolin A, et al: The natural killer cell—associated HNK—1 (Leu 7) antibody reacts with hypertrophic and malig—nant prostatic epithelium. Cancer 56:289–293, 1985.PubMedCrossRefGoogle Scholar
  177. 177.
    Miettinen M: Synaptophysin and neurofilament proteins as markers for neuroendocrine tumors. Arch Pathol Lab Med 111:813–818, 1987.PubMedGoogle Scholar
  178. 178.
    Gould VE, Wiedenmann B, Lee I, et al: Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am J Pathol 126:243–257, 1987.PubMedGoogle Scholar
  179. 179.
    Wiedenmann B, Kuhn C, Schwechheimer K, et al: Synaptophysin identified in metastases of neuroendocrine tumors by immunocytochemistry and immunoblotting. Am J Clin Pathol 88:560–569, 1987.PubMedGoogle Scholar
  180. 180.
    Gould VE: Histogenesis and differentiation: A re—evaluation of these concepts as criteria for the classification of tumors. Hum Pathol 17:212–215, 1986.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Mark R. Wick
  • Ross W. Simpson
  • Gloria A. Niehans
  • Bernd W. Scheithauer

There are no affiliations available

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