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Extraction and Analysis of Free and Protein-Bound Amino Acids from Norway Spruce Foliage

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Modern Methods in Forest Genetics

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Amino acids are basic constituents of all living things; they are the building blocks of proteins and are intermediates in metabolism. A forest geneticist will look at amino acid quality (types present) and quantity (amount) for a variety of reasons. Variation in the quality of amino acids may serve as gene markers, for clone identification, and as a means of measuring population dynamics. In addition, amino acids are one of the nutrients present in trees that can be used by tree pests and thus they have implications in breeding for insect and disease resistant varieties of trees.

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References

  • Adams, E.: Amino acid metabolism. Ann. Rev. Biochem. 31, 173–212 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Bailey, J.L.: Techniques in Protein Chemistry, 2nd edition. Amsterdam, London, New York: Elsevier Publishing Company 1967.

    Google Scholar 

  • Beck, S.D.: Resistance of plants to insects. Ann. Rev. Entomol. 10, 207–232 (1965).

    Article  Google Scholar 

  • Bergmeyer, H.U. (ed.): Methoden der enzymatischen Analyse, II. Auflage, pp. 1610–1682. Weinheim/Bergstr.: Verlag Chemie 1970.

    Google Scholar 

  • Blackburn, S.: Micro techniques for amino acid analysis and peptide separation based on high voltage electrophoresis. In: A Laboratory Manual of Analytical Methods of Protein Chemistry Including Polypeptides (eds. P. Alexander, H.P. Lundgren), Vol. IV, pp. 78–102. Oxford, London, Edinburgh, New York, Toronto, Paris, Frankfurt: Pergamon Press 1966.

    Google Scholar 

  • Block, R.J.: Amino acid analysis of protein hydrolysates. In: A Laboratory Manual of Analytical Methods of Protein Chemistry Including Polypeptides (eds. P. Alexander, R.J. Block), Vol. II, pp. 44–55. Oxford, London, New York, Paris: Pergamon Press 1960.

    Google Scholar 

  • Block, R.J., Durrum, E.L., Zweig, G.: A Manual of Paper Chromatography and Paper Electrophoresis, 2nd ed. New York, Academic Press 1958.

    Google Scholar 

  • Burchfield, H.P., Storrs, E.E.: Biochemical Applications of Gas Chromatography, pp. 573–588. New York, London: Academic Press 1962.

    Google Scholar 

  • Chibnall, A.C., Haselbach, C., Mangan, J.L., Rees, M.W.: Studies of the amide and C-terminal residues in proteins. 5. Estimation of asparagine and glutamine residues. Biochem. J. 68, 122–128 (1958).

    PubMed  CAS  Google Scholar 

  • Clotten, R., Clotten, A.: Hochspannungselektrophorese. Stuttgart: Georg-Thieme-Verlag 1962.

    Google Scholar 

  • Cramer, F.: Papierchromatographie, 4. Aufl. Weinheim/Bergstr.: Verlag Chemie 1958.

    Google Scholar 

  • Dadd, R.H.: Insect nutrition: Current developments and metabolic implications. Ann. Rev. Entomol. 18, 381–420 (1973).

    Article  CAS  Google Scholar 

  • Dorfner, K.: Ionenaustauscher, Eigenschaften and Anwendungen, 2. Aufl. Berlin: Walter de Gruyter 1964.

    Google Scholar 

  • Durz.an, D.J.: Nitrogen metabolism of Picea gZauca. I. Seasonal changes of free amino acids in buds, shoot apices, and leaves and the metabolism of uniformly labelled 14C-L-arginine by buds during the onset of dormancy. Can. J. Botany 46, 909–919 (1968a).

    Google Scholar 

  • Durzan, D.J.: Nitrogen metabolism of Picea glauca. II. Diurnal changes of free amino acids, amides, and guanidino compounds in roots, buds, and leaves during the onset of dormancy of white spruce saplings. Can. J. Botany 46, 921–928 (1968b).

    Article  CAS  Google Scholar 

  • Durzan, D.J.: Nitrogen metabolism of Picea glauca. III. Diurnal changes of amino acids, amides, protein, and chlorophyll in leaves of expanding buds. Can. J. Botany 46, 929–937 (1968c).

    Article  CAS  Google Scholar 

  • Durzan, D.J., Steward, F.C.: The nitrogen metabolism of Picea glauca (Moench) Voss and Pinus banksiana (Lamb.) as influenced by mineral nutrition. Can. J. Botany 45, 695–710 (1967).

    Article  CAS  Google Scholar 

  • El-Basyouni, S.Z., Neish, A.C., Towers, G.H.N.: The phenolic acids in wheat–III. Insoluble derivatives of phenolic cinnamic acids as natural intermediates in lignin biosynthesis. Phytochemistry 3, 627–639 (1964).

    Article  CAS  Google Scholar 

  • Feeny, P.: Seasonal changes in oak leaf tannins and nutrients as a cause of spring feeding by winter moth caterpillars. Ecology 51, 565–581 (1970).

    Article  Google Scholar 

  • Fowden, L.: The chemistry and metabolism of recently isolated amino acids. Ann. Rev. Biochem. 33, 173–204 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Hamilton, L.D.G.: The estimation of side chain groups in the intact protein. In: A Laboratory Manual of Analytical Methods in Protein Chemistry Including Polypeptides (eds. P. Alexander, R.J. Block ), Vol. II. Oxford, London, New York, Paris: Pergamon Press 1960.

    Google Scholar 

  • Hamilton, P.B.: Separation of amino acids. In: Ion Exchangers in Organic and Biochemistry (eds. C. Calmon, T.R.E. Kressman ), pp. 255–289. New York: Interscience Publ. Inc. 1957.

    Google Scholar 

  • Hess, G.P., Rupley, J.A.: Structure and function of proteins. Ann. Rev. Biochem. 40, 1013–1044 (1971).

    Article  PubMed  CAS  Google Scholar 

  • House, H.L.: Insect nutrition. Ann. Rev. Entomol. 6, 13–26 (1961).

    Article  Google Scholar 

  • Kaludin, K., Kaludin, I.: Untersuchungen über den Stickstoff-and Aminosäuregehalt in den Nadeln von Pinus silvestris. Arch. Forstw. 17, 839–844 (1968).

    CAS  Google Scholar 

  • Kawerau, E., Wieland, T.: Conservation of amino-acid chromatograms. Nature (London) 168, 77–78 (1951).

    Article  CAS  Google Scholar 

  • Lipke, H., Fraenkel, G.: Insect nutrition. Ann. Rev. Entomol. 1, 17–44 (1956).

    Article  Google Scholar 

  • Löyttyniemi, K., Tulisalo, U.: Amino acid composition of the needles of four Norway spruce provenances, and their effect on the occurrence of OZigonyohus ununguis (Jacobi) (Acarina, Tetranychidae). Ann. Ent. Fenn. 38, 122–126 (1972).

    Google Scholar 

  • Lück, H., Pavlik, A.: Die Aminosäuren-Zusammensetzung des Eiweißes rauchgeschädigter Fichtennadeln. Zeitschr. Pflanz. Ernähr. Düng. Bodenk. 114, 113–116 (1966).

    Article  Google Scholar 

  • Lunderstädt, J.: Zur Nahrungsqualität von Fichtennadeln für forstliche Schadinsekten. 3. fiber physiologisch bedingte Veränderungen in Fichtennadeln. Z. angew. Entomol. 73, 340–351 (1973).

    Google Scholar 

  • Lunderstädt, J., Claus, G.: Zur Nahrungsqualität von Fichtennadeln für forstliche Schadinsekten. 2. Vergleichende biochemische Analysen von Fichtennadeln and Kot der Larven von Gilpinia hercyniae Htg. Z. angew. Entomol. 70, 386–403 (1972).

    Google Scholar 

  • Materna, J., Ryskova, L.: Amino acids in Norway spruce. Comm. Inst. For. Cechosloveniae 4, 76–81 (1965).

    Google Scholar 

  • McFarren, E.F., Brand, K., Rutkowski, H.R.: Quantitative determination of sugars on filter paper chromatograms by direct photometry. Anal. Chem. 23, 1146–1149 (1951).

    Article  CAS  Google Scholar 

  • Moore, S., Stein, W.H.: Chromatography of amino acids on sulfonated polystyrene resins. J. Biol. Chem. 192, 663–681 (1951).

    PubMed  CAS  Google Scholar 

  • Painter, R.H.: Resistance of plants to insects. Ann. Rev. Entomol. 3, 267–290 (1958).

    Article  Google Scholar 

  • Pierce, A.E.: Silylation of organic compounds, pp. 218–243. Rockford, Illinois: Pierce Chemical Company 1968.

    Google Scholar 

  • Rees, M.W.: The estimation of threonine and serine in proteins. Biochem. J. 40, 632–639 (1946).

    PubMed  CAS  Google Scholar 

  • Rohringer, R., Fuchs, A., Lunderstädt, J., Samborski, D.J.: Metabolism of aromatic compounds in healthy and rust-infected primary leaves of wheat. I. Studies with 14CO2, Quinate-U-1 C, and Shikimate-U-14C as precursors. Can. J. Botany 45, 863–889 (1967).

    CAS  Google Scholar 

  • Rydon, H.N., Smith, P.W.G.: A new method for the detection of peptides and similar compounds on paper chromatograms. Nature (London) 169, 922–923 (1952).

    Article  CAS  Google Scholar 

  • Schroeder, W.A.: Separation of peptides. In: Ion Exchanges in Organic and Biochemistry (eds. C. Calmon, T.R.E. Kressman ), pp. 299–317. New York: Interscience Publ. Inc. 1957.

    Google Scholar 

  • Siren, G., Blombäck, B., Alden, T.: Proteins in forest tree leaves. Dept. of Reforestation, Royal College of Forestry, Stockholm, 28, 1–22 (1970).

    Google Scholar 

  • Smith, I.: Chromatographic and electrophoretic techniques, Vol. 1, Chromatography. London: Heinemann Medical Books Ltd. 1969.

    Google Scholar 

  • Sobel, A.E., Hirschman, A., Besman, L.: A convenient microtitration method for estimation of amino acids. J. Biol. Chem. 161, 99–103 (1945).

    PubMed  CAS  Google Scholar 

  • Spackman, D.H., Stein, W.H., Moore, S.: Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem. 30, 1190–1206 (1958).

    Article  CAS  Google Scholar 

  • Stegemann, H.: Bestimmung von Aminosäuren mit dithionitreduziertem Ninhydrin. Hoppe-Seylers Z. Physiol. Chem. 319, 102–109 (1960).

    Article  CAS  Google Scholar 

  • Thompson, J.F., Morris, C.J., Smith, J.K.: New naturally occurring amino acids. Ann. Rev. Biochem. 38, 137–158 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Thorsteinson, A.J.: Host selection in phytophagous insects. Ann. Rev. Entomol. 5, 193–218 (1960).

    Article  Google Scholar 

  • Vetter, W.: Amino acids. In: Biochemical Applications of Mass Spectrometry (ed. G.R. Waller ), pp. 387–404. New York, London, Sydney, Toronto: Wiley Interscience 1972.

    Google Scholar 

  • Walton, H.F.: Principles of ion exchange. In: Chromatography, 2nd edition (ed. E. Heftmann ), pp. 287–324. New York: Reinhold Publ. Corp. 1967a.

    Google Scholar 

  • Walton, H.F.: Techniques and applications of ion exchange chromatography. In: Chromatography, 2nd edition (ed. E. Heftmann ), pp. 325–342. New York: Reinhold Publ. Corp. 1967b.

    Google Scholar 

  • Wehrmann, J.: Methodische Untersuchungen zur Durchführung von Nadelanalysen in Kiefernbeständen. Forstw. Cbl. 78, 77–97 (1959a).

    Article  CAS  Google Scholar 

  • Wehrmann, J.: Die Mineralstoffernährung von Kiefernbeständen (Pinus silvestris) in Bayern. Forstw. Cbl. 78, 129–149 (1959b).

    Article  Google Scholar 

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Authors
  1. J. Lunderstädt
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Editors and Affiliations

  1. U.S.Department of Agriculture, Forest Service Institute of Forest Genetics, North Central Forest Experiment Station, P.O.Box 898, 54501, Rhinelander, WI, USA

    Jerome P. Miksche

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Lunderstädt, J. (1976). Extraction and Analysis of Free and Protein-Bound Amino Acids from Norway Spruce Foliage. In: Miksche, J.P. (eds) Modern Methods in Forest Genetics. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-12523-6_4

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  • DOI: https://doi.org/10.1007/978-3-662-12523-6_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-12525-0

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