A Na+,K+-ATPase inhibitor isolated from pig kidney
Pig kidney alcoholic extracts contain various substances which cross-react with antibodies for cardiac glycosides. Further fractions were found which inhibited Na+,K+-ATPase activity, displaced 3H-ouabain binding and had a positive inotropic effect on guinea-pig left atria.
The Na+,K+-ATPase inhibitory factors did not correlate with cardiac glycoside antibody cross-reactivity.
One fraction A4, which was radioimmunologically inert and extremely lipophilic, was not significantly contaminated with ions, showed a potent inhibition of the Na+,K+-ATPase, displaced 3H-ouabain binding and increased cardiac contractile force.
A further fraction, B1, was radioimmunologically active, but was found to inhibit the Na+,K+-ATPase, displace 3H-ouabain binding and increase contractile force due to a contamination with a significant amount of calcium.
KeywordsATPase Activity Contractile Force Cardiac Glycoside Positive Inotropic Effect Sodium Iodide
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- 4.Kim RS, LaBella FS (1981) Pharmac Ther 14: 39Google Scholar