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A Na+,K+-ATPase inhibitor isolated from pig kidney

  • M. Krekler
  • A. A. L. Fox
  • H. Strobach
  • K. Greeff
Conference paper

Summary

Pig kidney alcoholic extracts contain various substances which cross-react with antibodies for cardiac glycosides. Further fractions were found which inhibited Na+,K+-ATPase activity, displaced 3H-ouabain binding and had a positive inotropic effect on guinea-pig left atria.

The Na+,K+-ATPase inhibitory factors did not correlate with cardiac glycoside antibody cross-reactivity.

One fraction A4, which was radioimmunologically inert and extremely lipophilic, was not significantly contaminated with ions, showed a potent inhibition of the Na+,K+-ATPase, displaced 3H-ouabain binding and increased cardiac contractile force.

A further fraction, B1, was radioimmunologically active, but was found to inhibit the Na+,K+-ATPase, displace 3H-ouabain binding and increase contractile force due to a contamination with a significant amount of calcium.

Keywords

ATPase Activity Contractile Force Cardiac Glycoside Positive Inotropic Effect Sodium Iodide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Akera T, Fox AAL, Greeff K (1981) In: Greeff K (ed) Cardiac Glycosides Part 1. Experimental Pharmacology Handbook. Exp Pharm 56: 459, Springer-Verlag, BerlinCrossRefGoogle Scholar
  2. 2.
    Butler VP, Chen JP (1969) Proc Natl Acad Sci 57: 71CrossRefGoogle Scholar
  3. 3.
    Fox AAL, Greeff K (1981) Biochem Pharmacol 30: 611PubMedCrossRefGoogle Scholar
  4. 4.
    Kim RS, LaBella FS (1981) Pharmac Ther 14: 39Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • M. Krekler
    • 2
  • A. A. L. Fox
    • 1
  • H. Strobach
    • 1
  • K. Greeff
    • 1
  1. 1.Institut für PharmakologieUniversität DüsseldorfDüsseldorfGermany
  2. 2.Institut für Pharmakologie der UniversitätDüsseldorf 1West Germany

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