Cardiac energetics: significance of mitochondria

  • M. Siess

Summary

The mitochondrial activity as the energy producing step during biological oxidation was observed at rest and its regulation by the energy consuming auxotonic contractile work, depending on the preload, afterload and beat rate in isolated superfused left guinea pig atria. The mitochondrial activity was measured by (1) continuous determination of the O2 uptake rate, (2) the rate of 14CO2 production from labelled glucose or FFA and (3) separate measurements of the atrial ATP-, ADP-, AMP-, CP- and NAD-concentrations, for determination of the energy state. Some results, with points of general interest, are reported and discussed, including this model, former studies about cardiac energetics and the efficiency of cardiac work, reviewed recently [21].

Keywords

Ischemia Respiration Cytosol NADH Adrenaline 

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References

  1. 1.
    Bittl JA, Ingwall JS (1986) The energetics of myocardial stretch, creatine kinase flux and oxygen consumption in the noncontracting rat heart. Circ Res 58: 378–383PubMedCrossRefGoogle Scholar
  2. 2.
    Chance B, Williams GR (1956) Respiratory enzymes in oxidation phosphorylation. J Biol Chem 217: 409–427Google Scholar
  3. 3.
    Delabar U, Siess M (1979) Synthesis and degradation of NAD in guinea pig cardiac muscle: I. Dependence upon the extracellular concentration of nicotinamide and nicotinic acid. Basic Res Cardiol 74: 528–544Google Scholar
  4. 4.
    Delabar U, Siess M (1979) Synthesis and degradation of NAD in guinea pig cardiac muscle: II. Studies about the different biosynthetic pathways and the corresponding intermediates. Basic Res Cardiol 74: 571–593Google Scholar
  5. 5.
    Fiskum G, Lehninger AL (1982) Mitochondrial regulation of intracellular calcium. In: Wai Yiu Cheung (ed) Calcium and cell function, vol II. Academic Press, New York, pp 39–80Google Scholar
  6. 6.
    Gibbs CL (1983) Thermodynamics and cardiac energetics. In: Dintenfass L, Julian DG, Seaman GVF (eds) Heart perfusion, energetics and ischemia. Plenum Press, New York, NATO Scientific Affairs Division, Series A, vol 62, pp 549–576Google Scholar
  7. 7.
    Gibbs CL (1985) The cytoplasmatic phosphorylation potential. Its possible role in the control of myocardial respiration and cardiac contractility. J Mol Cell Cardiol 17: 727–731PubMedCrossRefGoogle Scholar
  8. 8.
    Giesen J, Kammermeier H (1980) Relationship of phosphorylation potential and oxygen consumption in isolated perfused rat hearts. J Mol Cell Cardiol 12: 891–907PubMedCrossRefGoogle Scholar
  9. 9.
    Kammermeier H, Schmid P, Jüngling E (1983) Free energy change of ATP hydrolysis: a causal factor of early hypoxic failure of the myocardium? J Mol Cell Cardiol 14: 267–277CrossRefGoogle Scholar
  10. 10.
    Loiselle DS (1985) The effect of temperature on the basal metabolism of cardiac muscle. Pflügers Arch 405: 163–169 (Europ J Physiol)Google Scholar
  11. 11.
    McCormack JG, Denton RM (1986) Cat+ ions as a link between functional demands and mitochondrial metabolism in the heart. In: Rupp H (ed) The regulations of heart function, basic concepts and clinical applications. Thieme Inc, New York, pp 186–200Google Scholar
  12. 12.
    Mommaerts WFHM (1970) What is the “Fenn”-effect? Muscle is a regulatory engine, the energy output of which is governed by the load. Naturwissenschaften 57: 326–330CrossRefGoogle Scholar
  13. 13.
    Rupp H (1986) The Ca+ + responsiveness of myofilaments in terms of ATPase activity, shortening velocity, and tension generation. In: Rupp H (ed) The regulation of heart function. Thieme Inc, New York, pp 234–248Google Scholar
  14. 14.
    Sheu S-S, Sharma VK, Uglesity A (1986) Na+—Ca++ exchange contributes to increase of cytosolic Ca + + concentration during depolarization in heart muscle. Ann J Physiol (Cell Physiol 19 ) 20: C651 — C656Google Scholar
  15. 15.
    Siess M (1977) Influences on the efficiency of cardiac work. Basic Res Cardiol 72: 299–305PubMedCrossRefGoogle Scholar
  16. 16.
    Siess M (1983) Influences on the mitochondrial function of cardiac tissue. In: Sono KH and Nagano M (eds) Cardiac structure and metabolism. Tokyo, pp 1–42Google Scholar
  17. 17.
    Siess M, Delabar U, Stieler K, Leuchtner J, Teutsch I, Khattab A, El Hawary MB (1987) Protective and nonprotective effects of drugs on cardiac contractile activity and high energy phosphates during anoxia and after reoxygenation. In: Dhalla NS, Innes IR, Beamish RE (eds) Myocardial ischemia. Martinus Nijhoff Publ, Boston Mass, USA, pp 20 (in press)Google Scholar
  18. 18.
    Siess M, Keller HJ, Scharre E, Geisler J, Müller G (1970) The continuous and simultaneous measurement of OZ consumption, rate of decarboxylation of 14C substrates and the performance of spontaneous beating isolated heart atria of guinea pigs. J Mol Cell Cardiol 1: 261289Google Scholar
  19. 19.
    Siess M, Mensing HJ, Stieler K (1976) Investigations about the determinants of the myocardial oxygen consumption. In: Knoll J, Szekeres L, Papp JGy (eds) Symposium on pharmacology of the heart. Akadémiai Kiado, Budapest, pp 65–73Google Scholar
  20. 20.
    Siess M, Stieler K (1984) Methods for studying mitochondrial function in superfused cardiac muscle preparations. In: Dhalla NS (ed) Methods in studying cardiac membranes, vol I, CRC Press Inc, Boca Raton, FL/USA, pp 87–109Google Scholar
  21. 21.
    Siess M, Stieler K, Leuchtner J, Delabar U (1986) Some problems of cardiac energetics. In: Jacob R (ed) (1986) Controversial issues in cardiac pathophysiology. Basic Res Cardiol [Suppl 1] 81: 79–94CrossRefGoogle Scholar
  22. 22.
    Zeitler N (1986) Untersuchungen zum Gehalt energiereicher Phosphate and NAD im Herzvorhof bei Kalium-Depolarisation. Inaug Dissertation Medizinische Fakultät ( Theoret Medizin ), Tübingen, pp 1–76Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • M. Siess
    • 1
  1. 1.Department of Pharmacology, Faculty of Theoretical MedicineUniversity of TübingenTübingenGermany

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