Summary
The mitochondrial activity as the energy producing step during biological oxidation was observed at rest and its regulation by the energy consuming auxotonic contractile work, depending on the preload, afterload and beat rate in isolated superfused left guinea pig atria. The mitochondrial activity was measured by (1) continuous determination of the O2 uptake rate, (2) the rate of 14CO2 production from labelled glucose or FFA and (3) separate measurements of the atrial ATP-, ADP-, AMP-, CP- and NAD-concentrations, for determination of the energy state. Some results, with points of general interest, are reported and discussed, including this model, former studies about cardiac energetics and the efficiency of cardiac work, reviewed recently [21].
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References
Bittl JA, Ingwall JS (1986) The energetics of myocardial stretch, creatine kinase flux and oxygen consumption in the noncontracting rat heart. Circ Res 58: 378–383
Chance B, Williams GR (1956) Respiratory enzymes in oxidation phosphorylation. J Biol Chem 217: 409–427
Delabar U, Siess M (1979) Synthesis and degradation of NAD in guinea pig cardiac muscle: I. Dependence upon the extracellular concentration of nicotinamide and nicotinic acid. Basic Res Cardiol 74: 528–544
Delabar U, Siess M (1979) Synthesis and degradation of NAD in guinea pig cardiac muscle: II. Studies about the different biosynthetic pathways and the corresponding intermediates. Basic Res Cardiol 74: 571–593
Fiskum G, Lehninger AL (1982) Mitochondrial regulation of intracellular calcium. In: Wai Yiu Cheung (ed) Calcium and cell function, vol II. Academic Press, New York, pp 39–80
Gibbs CL (1983) Thermodynamics and cardiac energetics. In: Dintenfass L, Julian DG, Seaman GVF (eds) Heart perfusion, energetics and ischemia. Plenum Press, New York, NATO Scientific Affairs Division, Series A, vol 62, pp 549–576
Gibbs CL (1985) The cytoplasmatic phosphorylation potential. Its possible role in the control of myocardial respiration and cardiac contractility. J Mol Cell Cardiol 17: 727–731
Giesen J, Kammermeier H (1980) Relationship of phosphorylation potential and oxygen consumption in isolated perfused rat hearts. J Mol Cell Cardiol 12: 891–907
Kammermeier H, Schmid P, Jüngling E (1983) Free energy change of ATP hydrolysis: a causal factor of early hypoxic failure of the myocardium? J Mol Cell Cardiol 14: 267–277
Loiselle DS (1985) The effect of temperature on the basal metabolism of cardiac muscle. Pflügers Arch 405: 163–169 (Europ J Physiol)
McCormack JG, Denton RM (1986) Cat+ ions as a link between functional demands and mitochondrial metabolism in the heart. In: Rupp H (ed) The regulations of heart function, basic concepts and clinical applications. Thieme Inc, New York, pp 186–200
Mommaerts WFHM (1970) What is the “Fenn”-effect? Muscle is a regulatory engine, the energy output of which is governed by the load. Naturwissenschaften 57: 326–330
Rupp H (1986) The Ca+ + responsiveness of myofilaments in terms of ATPase activity, shortening velocity, and tension generation. In: Rupp H (ed) The regulation of heart function. Thieme Inc, New York, pp 234–248
Sheu S-S, Sharma VK, Uglesity A (1986) Na+—Ca++ exchange contributes to increase of cytosolic Ca + + concentration during depolarization in heart muscle. Ann J Physiol (Cell Physiol 19 ) 20: C651 — C656
Siess M (1977) Influences on the efficiency of cardiac work. Basic Res Cardiol 72: 299–305
Siess M (1983) Influences on the mitochondrial function of cardiac tissue. In: Sono KH and Nagano M (eds) Cardiac structure and metabolism. Tokyo, pp 1–42
Siess M, Delabar U, Stieler K, Leuchtner J, Teutsch I, Khattab A, El Hawary MB (1987) Protective and nonprotective effects of drugs on cardiac contractile activity and high energy phosphates during anoxia and after reoxygenation. In: Dhalla NS, Innes IR, Beamish RE (eds) Myocardial ischemia. Martinus Nijhoff Publ, Boston Mass, USA, pp 20 (in press)
Siess M, Keller HJ, Scharre E, Geisler J, Müller G (1970) The continuous and simultaneous measurement of OZ consumption, rate of decarboxylation of 14C substrates and the performance of spontaneous beating isolated heart atria of guinea pigs. J Mol Cell Cardiol 1: 261289
Siess M, Mensing HJ, Stieler K (1976) Investigations about the determinants of the myocardial oxygen consumption. In: Knoll J, Szekeres L, Papp JGy (eds) Symposium on pharmacology of the heart. Akadémiai Kiado, Budapest, pp 65–73
Siess M, Stieler K (1984) Methods for studying mitochondrial function in superfused cardiac muscle preparations. In: Dhalla NS (ed) Methods in studying cardiac membranes, vol I, CRC Press Inc, Boca Raton, FL/USA, pp 87–109
Siess M, Stieler K, Leuchtner J, Delabar U (1986) Some problems of cardiac energetics. In: Jacob R (ed) (1986) Controversial issues in cardiac pathophysiology. Basic Res Cardiol [Suppl 1] 81: 79–94
Zeitler N (1986) Untersuchungen zum Gehalt energiereicher Phosphate and NAD im Herzvorhof bei Kalium-Depolarisation. Inaug Dissertation Medizinische Fakultät ( Theoret Medizin ), Tübingen, pp 1–76
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Siess, M. (1987). Cardiac energetics: significance of mitochondria. In: Jacob, R., Just, H., Holubarsch, C. (eds) Cardiac Energetics. Steinkopff, Heidelberg. https://doi.org/10.1007/978-3-662-11289-2_7
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DOI: https://doi.org/10.1007/978-3-662-11289-2_7
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