Anoxic injury of adult cardiac myocytes

  • H. M. Piper
  • P. Schwartz
  • R. Spahr
  • J. F. Hütter
  • P. G. Spieckermann
Conference paper

Summary

Cultured adult cardiocytes were exposed to anoxia. The initial decrease of high-energy phosphates was accompanied by a moderate release of cytosolic enzymes and morphological changes: the appearance of sarcolemmal ‘microblebs’ (~1 µm in diameter) and an increase of subsarcolemmal vesicles. At ATP levels above 2 µmol/gww, metabolic and morphological alterations were reversible. Probably the sarcolemmal changes are causally related to the loss of macromolecules from reversibly injured cells. At ATP levels below 2 µmol/gww, an increasing number of cells become irreversibly hypercontracted. In these cells cytoplasmic masses are protruded into large `macroblebs’ (10–30 µm in diameter), however sarcolemmal continuity is preserved. Thus, enzyme release, irreversible contracture and cytolysis do not occur simultaneously in anoxic isolated cardiocytes.

Key words

anoxia enzyme release cytolysis sarcolemmal blebbing adult cardiac myocytes 

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References

  1. 1.
    Ahmed SA, Williamson JR, Roberts R, Clark RE, Sobel BE (1976): The association of increased plasma MB CPK activity and irreversible ischemic myocardial injury in the dog. Circulation 54: 187–193PubMedCrossRefGoogle Scholar
  2. 2.
    Bergmeyer HU (1974): Methods of Enzymatic Analysis. Academic Press, New YorkGoogle Scholar
  3. 3.
    Ganote CE (1984): Contraction band necrosis and irreversible myocardial injury. J Moll Cell Cardiol 15: 67–73CrossRefGoogle Scholar
  4. 4.
    Gebhard MM, Denkhaus H, Sakai K, Spieckermann PG (1977): Energy metabolism and enzyme release. J Mol Med 2: 271–283Google Scholar
  5. 5.
    Piper HM, Probst I, Schwartz P, Hütter JF, Spieckermann PG (1982): Culturing of calcium stable adult cardiac myocytes. J Mol Cell Cardiol 14: 397–412.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • H. M. Piper
    • 2
  • P. Schwartz
    • 1
  • R. Spahr
    • 1
  • J. F. Hütter
    • 1
  • P. G. Spieckermann
    • 1
  1. 1.Zentrum Physiologie und Zentrum AnatomieUniversität GöttingenGöttingenGermany
  2. 2.Zentrum Physiologie der UniversitätGöttingenGermany

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