Guinea-pig atrial cardioballs

  • M. Bechem
  • F. Pieper
  • L. Pott
Conference paper


Myocytes from atria of adult guinea-pigs were isolated by means of a previously described enzyme perfusion with some modifications.

The problem of Ca-intolerance of the dispersed cells was circumvented by (i) avoiding cooling of the cells below 25 °C and (ii) increasing the Ca concentration slowly already during the enzyme perfusion.

Isolated atrial myocytes were taken in long-term cell culture. Under this condition they become spherical within about 2 days. The rounded stage (cardioballs), which is found in the cultures for a period of ca. 10 days is highly suited for electrophysiological studies using the different recording configurations of the patch clamp technique, including ‘tight-seal whole-cell recording’ with simultaneous cell dialysis.

Key words

single heart cell cardioball cell culture whole-cell recording 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • M. Bechem
    • 2
  • F. Pieper
    • 1
  • L. Pott
    • 1
  1. 1.Institut für ZellphysiologieRuhr-Universität BochumBochumGermany
  2. 2.Institut für PharmakologieBayer ForschungszentrumWuppertalGermany

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