Wheat pp 659-668 | Cite as

Transient Gene Expression in Wheat (Triticum aestivum) Protoplasts

  • B. T. Lee
  • K. Murdoch
  • J. Topping
  • M. Kreis
  • M. G. K. Jones
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


Recent advances in methods of genetic transformation of plants have resulted in the introduction, stable integration, and heritable transmission of foreign genes in a number of species. However, the economically important cereals, which include bread wheat (Triticum aestivum), are less amenable to these techniques, and heritable transmission of introduced genes into wheat has not yet been reported. One approach that can be applied for introduction of foreign genes into both dicot (Krens et al. 1982; Paszkowski et al. 1984) and monocot species (Potrykus et al. 1985; Lörz et al. 1985) is direct transfer of DNA constructs into protoplasts.


Triticum Aestivum Transient Expression Transient Gene Expression Direct Gene Transfer Suspension Protoplast 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. T. Lee
  • K. Murdoch
  • J. Topping
  • M. Kreis
  • M. G. K. Jones
    • 1
  1. 1.Rothamsted Experimental StationHertsUK

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