Advertisement

Wheat pp 659-668 | Cite as

Transient Gene Expression in Wheat (Triticum aestivum) Protoplasts

  • B. T. Lee
  • K. Murdoch
  • J. Topping
  • M. Kreis
  • M. G. K. Jones
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)

Abstract

Recent advances in methods of genetic transformation of plants have resulted in the introduction, stable integration, and heritable transmission of foreign genes in a number of species. However, the economically important cereals, which include bread wheat (Triticum aestivum), are less amenable to these techniques, and heritable transmission of introduced genes into wheat has not yet been reported. One approach that can be applied for introduction of foreign genes into both dicot (Krens et al. 1982; Paszkowski et al. 1984) and monocot species (Potrykus et al. 1985; Lörz et al. 1985) is direct transfer of DNA constructs into protoplasts.

Keywords

Triticum Aestivum Transient Expression Transient Gene Expression Direct Gene Transfer Suspension Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Callis J, Fromm M, Walbot V (1987) Introns increase gene expression in cultured maize cells. Genes Dev 1: 1183–1200PubMedCrossRefGoogle Scholar
  2. Fromm M, Taylor LP, Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82: 5824–5828PubMedCrossRefGoogle Scholar
  3. Gorman CM, Moffat LF, Howard BH (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2: 1044–1051PubMedGoogle Scholar
  4. Hauptmann RM, Ozias-Akins P, Vasil V, Tabaeizadeh Z, Rogers SG, Horsch RB, Vasil IK, Fraley RT (1987) Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep 6: 265–270CrossRefGoogle Scholar
  5. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231CrossRefGoogle Scholar
  6. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EM BO J 6: 3901–3907Google Scholar
  7. Jones H, Tempelaar MJ, Jones MGK (1987) Recent advances in plant electroporation. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol 4. Univ Press, Oxford, pp 347 357Google Scholar
  8. Junker B, Zimney J, Lührs R, Lörz H (1987) Transient expression of chimaeric genes in dividing and non-dividing cereal protoplasts after PEG-induced DNA uptake. Plant Cell Rep 6: 329–332CrossRefGoogle Scholar
  9. Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at very low population density in liquid media. Planta 126: 105–110CrossRefGoogle Scholar
  10. Krens FA, Molendijk L, Wullums GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature (London) 296: 72–74CrossRefGoogle Scholar
  11. Lee BT, Murdoch K, Topping J, Kreis M, Jones MGK (1989) Transient gene expression in aleurone protoplasts from developing caryopses of barley and wheat. Plant Mol Biol 13: 21–29PubMedCrossRefGoogle Scholar
  12. Lörz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol Gen Genet 199: 178–182CrossRefGoogle Scholar
  13. Maddock SE (1987) Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L.). Plant Cell Rep 6: 23–26CrossRefGoogle Scholar
  14. Negrutiu I, Shillito R, Potrykus 1, Biasini G, Sala F (1987) Hybrid genes in the analysis of transformation conditions. Plant Mol Biol 8: 363–373CrossRefGoogle Scholar
  15. Ou-Lee TM, Turgeon R, Wu R (1986) Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat and sorghum. Proc Natl Acad Sci USA 83: 6815–6819PubMedCrossRefGoogle Scholar
  16. Ow DW, Wood KV, DeLuca M, de Wet JR, Helinski DR, Howell SH (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856–859PubMedCrossRefGoogle Scholar
  17. Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3: 2717–2722PubMedGoogle Scholar
  18. Potrykus I, Saul M, Petruska J, Paszkowski J, Shillito RD (1985) Direct gene transfer to cells of a graminaceous monocot. Mol Gen Genet 199: 183–188CrossRefGoogle Scholar
  19. Reiss B, Sprengel R, Will H, Schaller H (1984) A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts. Gene 30: 211–218PubMedCrossRefGoogle Scholar
  20. Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338: 274–276CrossRefGoogle Scholar
  21. Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology 6: 1072–1074CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. T. Lee
  • K. Murdoch
  • J. Topping
  • M. Kreis
  • M. G. K. Jones
    • 1
  1. 1.Rothamsted Experimental StationHertsUK

Personalised recommendations