Abstract
In vitro approaches offer very powerful new tools for plant improvement (see Bajaj 1986a). However, reliable and highly efficient procedures for regeneration of plants from cultured tissues, cells and protoplasts are a prerequisite for applying these new techniques to agriculture, horticulture and forestry. As wheat is the most important crop in the world, and has the greatest harvested area and total production (FAO 1985), consistent procedures of plant regeneration from cultured tissue and protoplasts of wheat are of special significance.
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References
Abe T, Futsuharay (1985) Efficient plant regeneration by somatic embryogenesis from root callus tissues of rice (Oryza saliva L.). J Plant Physiol 121: 111–118
Agache S, De Buyser J, Henry Y, Snape JW (1988) Studies of the genetic relationship between anther cultures and somatic tissue culture abilities in wheat. Plant Breed 100: 26–33
Ahloowalia BS (1982) Plant regeneration from callus culture in wheat. Crop Sci 22:405–410
Ahloowalia BS, Sherington J (1985) Transmission of somaclonal variation in wheat. Euphytica 34: 525–537
Ahuja PS, Pental D, Cocking EC (1982) Plant regeneration from leaf base callus and cell suspensions of Triticum aestivum. Z Pflanzenzucht 89: 139–144
Bajaj YPS (1984) Regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor Appl Genet 67: 525–528
Bajaj YPS (ed) (1986a) Biotechnology in agriculture and forestry, vol 1: Trees I; vol 2: Crops I. Springer, Berlin Heidelberg New York Tokyo
Bajaj YPS (1986b) In vitro regeneration of diverse plants and the cryopreservation of germplasm in wheat (Triticum aestivum L.). Cereal Res Commun 14: 305–311
Bajaj YPS, Gosal SS (1986) Biotechnology of wheat improvement. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2: Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 3–38
Bennici A, Caffari L, Dameri RM, Gastaldo P, Profumo P (1988) Callus formation and plant regeneration from immature Triticum durum Desf. embryos. Euphytica 39: 255–263
Bhojwani SS, Hayward C (1977) Some observations and comments on tissue culture of wheat. Z Pflanzenphysiol 85: 341–347
Botti L, Vasil IK (1983) Plant regeneration by somatic embryogenesis from parts of cultured mature embryos of Pennisetum americanum ( L) K Schum. Z Pflanzenphysiol 111: 319–325
Boyd LA, Dale PJ (1986) Callus production and plant regeneration from mature embryos of Poa pratensis L. Plant Breed 97: 246–254
Brown C, Brooks FJ, Pearson D, Mathias RJ (1989) Control of embryogenesis and organogenesis in immature wheat embryo callus using increased medium osmolarity and abscisic acid. J Plant Physiol 133: 727–733
Butenko RG, Dzhardemaliev K (1986) Callus forming ability in explants of different organs of winter wheat varieties. Sov Plant Physiol 33: 277–281
Carman JG, Jefferson NE, Campbell WF (1987a) Induction of embryogenic Triticum aestivum L. calli. Quantification of genotype and culture medium effects. Plant Cell Tissue Organ Cult 10: 101–113
Carman JG, Jefferson NE, Campbell WF (1987b) Induction of embryogenic Triticum aestivum L. calli. Quantification of organic addenda and other culture variable effects. Plant Cell Tissue Organ Cult 10: 114–128
Chen THH, Larzar MD, Scole Gj, Gusta LV, Kartha KK (1987) Somaclonal variation in a population of winter wheat. J Plant Physiol 130: 127–136
Chin JC, Scott KJ (1977) Studies on the formation of roots and shoots in wheat callus cultures. Ann Bot (London) 41: 473–481
Chu CC, Sun CS, Chin X, Zhang WX, Du ZH (1984) Somatic embryogenesis and plant regeneration in callus from inflorescences of Hordeum vulgare X Triticum aestivum hybrids. Theor Appl Genet 68: 375–380
Conger BV, Hillenski LL, Lowe KW, Carabia JV (1982) Influence of different auxins at varying concentrations on callus induction and growth from embryos and leaf tip explants in Gramineae. Environ Exp Bot 22: 39–48
Cure WW, Mott RL (1978) A comparative anatomical study of organogenesis in cultured tissues of maize, wheat and oat. Physiol Plant 42: 91–96
De Buyser J, Hartmann C, Henry Y, Rode A (1988) Variations in long-term wheat somatic tissue culture. Can J Bot 66: 1891–1895
Dudits D, Nemet G, Haydu Z (1975) Study of callus growth and organ formation in wheat (Triticum aestivum) tissue culture. Can J Bot 53: 957–963
Evans DA (1983) Protoplast fusion. In: Evans DA, Sharp WR,Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1. MacMillan, New York, pp 291–321
FAO (ed) (1985) Production yearbook, vol 39. FAO, Rome
Felsenburg T, Feldman M, Galun E (1987) Aneuploid and alloplasmic lines as tools for the study of nuclear and cytoplasmic control of culture ability and regeneration of scutellum calli from common wheat. Theor Appl Genet 74: 802–810
Galiba G, Yamada Y (1988) A novel method for increasing the frequency of somatic embryogenesis in wheat tissue cultures by NaCI and KC1 supplementation. Plant Cell Rep 7: 55–58
Galiba G, Kovacs G, Sutka J (1986) Substitution analysis of plant regeneration from callus culture in wheat. Plant Breed 97: 261–263
Gosch-Wackerle G, Avivi L, Galun E (1979) Induction, culture and differentiation of callus from immature rachises, seeds and embryos of Triticum. Z Pflanzenphysio 191: 267–278
Greco B, Tanzarella OA, Blanco A (1984) Plant regeneration from leaf base callus in durum wheat (Triticum durum Desf.). Cereal Res Commun 12: 171–177
Haccius B (1978) Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology 28: 74–81
Harris R, Wright M, Byrne M, Varnum J, Brightwell B, Schubert K (1988) Callus formation and plantlet regeneration from protoplasts derived from suspension cultures of wheat (Triticum aestivum L.). Plant Cell Rep 7: 337–340
Hayashi Y, Shimamoto K (1988) Wheat protoplast culture: embryogenic colony formation from protoplasts. Plant Cell Rep 7: 414–417
He DG (1989) Embryo and protoplast culture of wheat (Triticum spp). PhD Thesis, The Queensland University
He DG, Ouyang JW (1984) Callus and plantlet formation from cultured wheat anthers at different developmental stages. Plant Sci Lett 33: 71–79
He DG, Ouyang JW (1985) Observation of androgenesis in cultured wheat anthers at meiosis, early uninucleate and trinucleate stage. Acta Bot (Beijing) 27: 469–475
He DG, Tanner G, Scott KJ (1986) Somatic embryogenesis and morphogenesis in callus derived from the epiblast of immature embryos of wheat (Triticum aestivum). Plant Sci 45: 119–124
He DG, Yang YM, Dahler G, Scott KJ (1988) A comparison of epiblast callus and scutellum callus induction in wheat: the effect of embryo age, genotype and medium. Plant Sci 57: 225–233
He DG, Yang YM, Scott KJ (1989) The effect of macroelements in the induction of embryogenic callus from immature embryos of wheat (Triticum aestivum L.). Plant Sci 65: (Ãn press)
HeyserJW, Nabors MW, MacKinnon C, Dykes TA, Demott DC, Kantzmann DC, Mujeb-Kazi A (1985) Long-term, high-frequency plant regeneration and the induction of somatic embryogenesis in callus cultures of wheat (Triticum aestivum L.). Z Pflanzenzucht 94: 218–233
Hunsinger H. Schauz K (1987) The influence of dicamba on somatic embryogenesis and frequency of plant regeneration from cultured immature embryos of wheat (Triticum aestivum L.). Plant Breed 98: 119–123
Jones MGK (1985) Cereal protoplast. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk, Dordrecht Boston Lancaster, pp 204–230
Karp A, Maddock SE (1984) Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor Appl Genet 67: 249–255
Kawata S, Ishihara A (1968) The regeneration of rice plant Oryza sativa L. in the callus derived from the seminal root. Proc Jpn Acad 44: 549–553
King PJ, Potrykus I, Thomas E (1978) In vitro genetics of cereals: problems and perspectives. Physiol Veg 16: 381–399
Larkin PJ, Scowcroft WA (1981) Somaclonal variation — a novel source of viability from cell cultures of plant improvement. Theor Appl Genet 60: 197–214
Larkin PJ, Ryan SA, Brettel RIS, Scowcroft WA (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67: 443–455
Lazar MD, Collins GB, Viars WE (1983) Genetic and environmental effects on the growth and differentiation of wheat Triticum aestivum somatic cell cultures. J Hered 74: 353–357
Lazar MD, Chin THH, Scoles GJ, Kartha KK (1987) Immature embryo and anther culture of chromosome addition lines of rye in Chinese Spring wheat. Plant Sci 51: 77–81
Li XH, Shu YR, Chen J, Zhu Z, Wang HB, Fang R, Wang P, Wei JQ (1989) Protoplast culture of wheat. Scientia Sinica (in press)
Lowe K, Taylor DB, Ryan P, Paterson KE (1985) Plant regeneration via organogenesis and embryogenesis in the maize inbred line B73. Plant Sci 41: 125–132
Lu C, Vasil IK (1982) Somatic embryogenesis and plant regeneration in tissue cultures of Panicum maximum Jacq. Am J Bot 69: 77–81
Lu C, Vasil IK, Ozias-Akins P (1982) Somatic embryogenesis in Zea mays L. Theor Appl Genet 62: 109–112
Lupi MC, Bennici A, Baroncelli S, Gennai D, D’Amato F (1981) In vitro regeneration of Durum wheat plants. II. diplontic selection plants. Z Pflanzenzücht 87: 167–171
Maddock SE (1985) Cell culture, somatic embryogenesis and plant regeneration in wheat, barley, oats, rye and triticale. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk, Dordrecht Boston Lancaster, pp 131–174
Maddock SE (1987) Suspension and protoplast culture of hexaploid wheat (Triticum aestivum L). Plant Cell Rep 6: 23–26
Maddock SE, Lancaster VA, Risiott R, Franklin J (1983) Plant regeneration from cultured immature embryos and inflorescences of 25 cultivars of wheat (Triticum aestivum). J Exp Bot 34: 915–926
Magnusson I, Bornmann CH (1985) Anatomical observation on somatic embryogenesis from scutellar tissues of immature zygotic embryos of Triticum aestivum. Physiol Plant 63: 137–145
Mascarenhas AF, Pathak M, Hendre RR, Ghugale DD, Jagannathan V (1975) Tissue culture of maize, wheat, rice and sorghum, pt 4: Studies of organ differentiation in tissue culture of maize, wheat and rice. Indian J Exp Biol 13: 116–119
Mathias RJ, Fukui K (1987) The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus. Theor Appl Genet 71: 797–800
Mathias RJ, Simpson ES (1986) The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L. em. thell) callus. Plant Cell Tissue Organ Cult 7: 31–37
McHughen A (1983) Rapid regeneration of wheat in vitro. Ann Bot 51: 851–853
Morris PC, Maddock SE, Jones MGK, Bowles DJ (1986) Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Rep 5: 460–463
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nabors MW, Heyser JW, Pykes TA, Mott KJ (1983) Long duration, high-frequency plant regeneration from cereal tissue culture. Planta 157: 385–391
Norstog K (1970) Induction of embryo-like structures by kinetin in cultured barley embryos. Dev Biol 23: 665–670
O’Hara JF, Street HE (1978) Wheat callus culture: the initiation, growth and organogenesis of callus derived from various explant sources. Ann Bot (London) 42: 1029–1038
Ohnoutkova L, Novak FJ, Ohnoutka Z (1984) In vitro embryogenesis and plant regeneration of wheat (Triticum aestivum L.). In: Novak FJ, Havel L, Dolezel J (eds) Int Symp Plant tissue and cell culture application to crop improvement, Prague, pp 107–109
Orton TJ (1985) Somaclonal variation: theoretical and practical consideration In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum, New York, pp 427–468
Ozias-Akins P, Vasil IK (1982) Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): Evidence for somatic embryogenesis. Protoplasma 110: 95–105
Ozias-Akins P, Vasil IK (1983a) Proliferation of and plant regeneration from epiblast of Triticum aestivum (wheat; Gramineae) embryos. Am J Bot 70: 1092–1097
Ozias-Akins P, Vasil IK (1983b) Callus induction and growth from the mature embryo of Triticum aestivum (wheat). Protoplasma 115: 104–113
Ozias-Akins P, Vasil IK (1983c) Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117: 40–44
Papenfuss JM, Carman JG (1987) Enhanced regeneration from wheat callus cultures using dicamba and kinetin. Crop Sci 27: 588–593
Percival J (1921) The wheat plant. Duckworth, London
Purnhauser L, Medgyesty P, Czako M, Dix PJ, Marton L (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia viv. tissue cultures using the ethylene inhibitor AgNO.,. Plant Cell Rep 6: 1–4
Raina SK, Sathis P, Sarma KS (1987) Plant regeneration from in vitro cultures of anthers and mature seeds of rice (Oryza sativa L.) cv. Basmati-370. Plant Cell Rep 6: 43–45
Rajyalakshimi K, Dhir SK, Maheshwari N, Maheshwari SC (1988) Callusing and regeneration of plantlets via somatic embryogenesis from inflorescence cultures of Triticum aestivum L.: role of genotype and long-term retention of morphogenic potential. Plant Breed 101: 80–85
Ram NV, Nabors MW (1985) Plant regeneration from tissue cultures of Pokkali rice is promoted by optimizing callus to medium volume ratio and by a medium-conditioning factor produced by embryogenic callus. Plant Cell Tissue Organ Cult 4: 241–248
Regel Z (1987) Embryogenic callus induction and plant regeneration from cultured Hordeum volgare mature seeds. Plant Physiol Biochem 25: 43–48
Ryan SA. Scowcroft WR (1987) A somaclonal variant of wheat with additional ’3-amylase isozymes. Theor Appl Genet 73: 459–464
Sala C. Biasini MG. Morandi C, Nielsen E. Parisi B. Sala F (1985) Selection and nuclear DNA analysis of cell hybrids between Daucus carota and Oryza sativa. J Plant Physiol 118: 409–419
Schaeffer GW, Lazar MD, Baenziger PS (1984) Wheat. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2. MacMillan, New York, pp 108–136
Sears RG, Deckard EL (1982) Tissue culture variability in wheat: callus induction and plant regene-ration. Crop Sei 22: 546–556
Shimada T (1978) Plant regeneration from the callus induced from wheat embryos. Jpn J Genet 53: 371–374
Shimada T, Yamada Y (1979) Wheat plant regenerated from embryo cell cultures. Jpn J Genet 54: 379–385
Street HE, Withers LA (1974) The anatomy of embryogenesis in culture. In: Street HE (ed) Tissue culture and plant science 1974. Academic Press, London, pp 71–100
Swift JG, O’Brien TP (1970) Vascularization of the scutellum of wheat. Aust Bot 18: 45–53
Thomas E. King PJ, Potrykus I (1977) Shoot and embryo-like structure formation from cultured tissues of Sorghum bicolor. Naturwissenschaften 64: 587
Thomas E, Brettell R, Potrykus I, Wernicke W (1980) Cereal tissue culture. In: Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Blackwell, Oxford, pp 41–49
Thomas MR, Scott KJ (1985) Plant regeneration by somatic embryogenesis from callus initiated from immature embryos and immature inflorescences of Hordeum vulgare. J Plant Physiol 121: 159–169
Tomes DT (1985) Cell culture, somatic embryogenesis: plant regeneration in maize, rice, sorghum and millet. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff/Junk, Dordrecht Boston Lancaster, pp 175–203
Vasil IK (1987) Developing cell and tissue culture system for improvement of cereal and grass crops. J Plant Physiol 128: 193–218
Vasil V, Vasil IK (1981) Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum and P. americanum X P. purpureum hybrid. Am J Bot 68: 864–872
Vasil V, Vasil IK (1982) Characterisation of an embryogenic cell suspension culture derived from inflorescences of Pennisetum americanum (pearl millet, Gramineae ). Am J Bot 69: 1441–1449
Vasil V, Vasil IK (1984) Isolation and culture of embryogenic protoplasts of cereals and grasses. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. vol 1. Academic Press, Orlando. pp 389–403
Vasil V, Lu CY, Vasil IK (1983) Proliferation and plant regeneration from the nodal region of Zea mays maize Gramineae embryos. Am J Bot 70: 951–954
Wang MS, Zapata J, De Castro DC (1987) Plant regeneration through embryogenesis from mature seed and young inflorescence of wild rice (Oryza perennis Moench). Plant Cell Rep 6: 294–296
Wernicke W, Milkovits L (1984) Developmental gradients in wheat leaves — response of leaf segments in different genotypes cultured in vitro. J Plant Physiol 115: 49–58
Wernicke W, Milkovits L (1986) The regeneration potential of wheat shoot meristems in the presence and absence of 2,4-dichlorophenoxyacetic acid. Protoplasma 131: 131–141
Wernicke W, Milkovits L (1987) Effect of auxin on the mitotic cell cycle in cultured leaf segments at different stages of development in wheat. Physiol Plant 69: 16–22
Wernicke W, Potrykus I, Thomas E (1982) Morphogenesis from cultured leaf tissue of Sorghum bicolor — the morphogenic pathways. Protoplasma 111: 53–62
Wernicke W,GorstJ, Milkovits L (1986) The ambiguous role of 2,4-dichlorophenoxyaceticacid in wheat tissue culture. Physiol Plant 68: 597–602
Wilke-Douglas W, Perani L, Radke S, Boossert M (1986) The application of recombinant DNA technology toward crop improvement. Physiol Plant 68: 560–565
Williams EG, Maheswaran G (1986) Somatic embryogenesis: factors influencing coordinated behaviour of cells as an embryogenic group. Ann Bot (London) 57: 443–462
Yurkova GN, Levenko BA, Novozhilov O (1981) Induction of plant regeneration in wheat tissue culture. Biochem Physiol Pflanzen 176: 236–243
Zamora AB, Scott KJ (1983) Callus formation and plant regeneration from wheat leaves. Plant Sci Lett 29: 183–189
Zhang Li, Seilleur P (1987) A simple and fast method to obtain high frequency of plant regeneration from mature and immature wheat embryos. Bull Rech Agron Gembloux 22: 187–197
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Scott, K.J., He, D.G., Yang, Y.M. (1990). Somatic Embryogenesis in Wheat. In: Bajaj, Y.P.S. (eds) Wheat. Biotechnology in Agriculture and Forestry, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10933-5_3
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