Wheat pp 403-415 | Cite as

Wheat Anther Culture: Effect of Temperature

  • B. Huang
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


Much effort has been made in the past to improve the efficiency of anther culture in wheat, and to apply the technique in breeding practice (Hu 1986; Henry and de Buyser 1986; Hu and Huang 1987). To date, anther culture has been the most efficient means of production of haploid and homozygous diploid in wheat, and, potentially, productivity of wheat anther culture can be manyfold higher than has been achieved so far. This chapter reviews some recent progress made in improving the efficiency of wheat (Triticum aestivum L.) anther culture, particularly by manipulating temperatures for pretreatment and anther culture, and speculates on modes of action of temperature treatment in wheat anther culture. Possible approaches to further optimize procedures of wheat anther culture and to better understand pollen development in vitro are discussed.


Anther Culture Culture Temperature Donor Plant Pollen Plant Cold Pretreatment 
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  1. Bajaj YPS (1977) In vitro induction of haploids in wheat (Triticum aestivum L.). Crop Improv 4:54–64 Bajaj YPS (1983) Cryopreservation of germplasm of cereals — progress and prospects. In: Proc 6th Int Wheat genetic Symp, Kyoto Univ, pp 565–574Google Scholar
  2. Bajaj YPS (1984) The regeneration of plants from frozen pollen embryos and zygotic embryos of wheat and rice. Theor Appl Genet 67: 525–528CrossRefGoogle Scholar
  3. Clapham DH (1973) Haploid Hordeum plants from anthers in vitro. Z Pflanzenzucht 69: 142–155Google Scholar
  4. Day A, Ellis THN (1984) Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell 39: 359–368PubMedCrossRefGoogle Scholar
  5. Day A, Ellis THN (1985) Deleted forms of plastid DNA in albino plants from cereal anther culture. Curr Genet 9: 671–678CrossRefGoogle Scholar
  6. Dunwell JM, Cornish M, DeCourcel AGL, Middlefell-Williams JE (1983) Induction and growth of `microspore-derived’ embryos of Brassica napus ssp. oleifera. J Exp Bot 34: 1768–1778CrossRefGoogle Scholar
  7. Henry Y, de Buyser J (1986) Wheat: production of haploids, performance of doubled haploids and yield trials. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2: Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 73–84Google Scholar
  8. Hu H (1986) Wheat: Improvement through anther culture. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2: Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 55–72Google Scholar
  9. Hu H, Huang B (1987) Application of pollen-derived plants to crop improvement. Int Rev Cytol 107: 293–313CrossRefGoogle Scholar
  10. Huang B (1985a) Influence of cold pretreatment on the production of pollen callus in anther cultures. Acta Bot Sin 27: 439–444Google Scholar
  11. Huang B (1985b) Albinism in pollen plants of Gramineae species (Review). Hereditas 7: 1–5Google Scholar
  12. Huang B (1987) Effects of incubation temperature on microspore callus production and plant regeneration in wheat anther cultures. Plant Cell Tissue Org Cult 9: 45–48CrossRefGoogle Scholar
  13. Huang B, Sunderland N (1982) Temperature stress pretreatment in barley anther culture. Ann Bot (London) 49: 77–88Google Scholar
  14. Keller WA, Armstrong KC (1978) High frequency production of microspore-derived plants from Brassica napus anther culture. Z Pflanzenzucht 80: 100–108Google Scholar
  15. Keller WA, Armstrong KC (1979) Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatment. Theor Appl Genet 55: 65–67CrossRefGoogle Scholar
  16. Keller WA, Armstrong KC, De LaRoche AI (1983) The production and utilization of microspore-derived haploids in Brassica crops. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum, New York, pp 169–183Google Scholar
  17. Lazar MD, Schaeffer GW, Baenzinger PS (1985) The physical environment in relation to high frequency callus and plantlet development in anther cultures of wheat (Triticum aestivum L.) cv. Chris. J Plant Physiol 121: 103–109Google Scholar
  18. Marsolais AA, Seguin-Swartz G, Kasha KJ (1984) The influence of anther cold pretreatments and donor plants genotypes on in vitro androgenesis in wheat (Triticum aestivum L.). Plant Cell Tissue Org Cult 3: 69–79CrossRefGoogle Scholar
  19. Nitsch C, Norreel B (1973) Effet d’un choc thermique sur le pouvior embryogene du pollen de Datura innoxia cultivé dans l’anthere ou isole de l’anthere. C R Acad Sci Paris Ser D 276: 303–306Google Scholar
  20. Ouyang JW, Shou SM, Jia SE (1983) The response of anther culture to culture temperature in Triticum aestivum. Theor Appt Genet 66: 101–109Google Scholar
  21. Ouyang JW, He DG, Feng GH, Jia SE (1987) The response of anther culture to culture temperature varies with growth conditions of anther-donor plants. Plant Sci 49: 145–148CrossRefGoogle Scholar
  22. Pan CL, Kao KH (1978) The production of wheat pollen embryo and the influence of some factors in its frequency of induction. In: Proc Symp Plant tissue culture, Peking. Science Press, Peking, pp 133–143Google Scholar
  23. Rashid A (1983) Pollen dimorphism in relation to pollen plant formation. Physiol Plant 58:544–548 Sangwan RS, Sangwan-Norreel BS (1987) Biochemical cytology of pollen embryogenesis. Int Rev Cytol 107: 221–272Google Scholar
  24. Sun CS, Wu SC, Wang CC, Chu CC (1979) The deficiency of soluble proteins and plastid ribosomal RNA in the albino pollen plantlets of rice. Theor Appl Genet 55: 193–197CrossRefGoogle Scholar
  25. Sunderland N, Huang B (1985) Barley anther culture— the switch of programme and albinism. Hereditas Suppl 3: 27–40Google Scholar
  26. Sunderland N, Huang B, Hills GJ (1984) Disposition of pollen in situ and its relevance to anther/pollen culture. J Exp Bot 35: 521–530CrossRefGoogle Scholar
  27. Wang CC, Sun CS, Chu CC (1977) Effects of culture factors in vitro on the production of albino pollen plantlets of rice. Acta Bot Sin 19: 190–199Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • B. Huang
    • 1
  1. 1.Allelix Crop TechnologiesMississaugaCanada

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