In Vitro Culture of Wheat Ovules
Small globular embryos fail to survive after transfer to media which normally support the growth of those embryos which already possess differentiated organs. Efforts to culture the globular embryos of members of the Gramineae, with the exception of Hordeum vulgare, have been unsuccessful (Norstog 1967). Examples in the literature document that ovule culture can be much more successful than embryo culture. The method of in vitro culture of ovules has the following advantages: (1) It is much easier to culture the whole ovules instead of isolating single globular embryos, especially in small seeded species; (2) globular embryos are less damaged when ovules are transferred into the media, as they are not removed from their natural environment; (3) the globular embryos can still be supplied by endogenous and exogenous nutrients; (4) transfer of ovules enables the culture of hybrid globular embryos at early stages of embryogenesis, that is before they start to degenerate.
KeywordsSucrose Agar Agarose Glycine Thiamine
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