Wheat pp 250-261 | Cite as

Embryo Culture of Wheat — Regenerative Tissue Culture System

  • T. Shimada
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 13)


Embryo culture has often been applied to practical problems in plant breeding. For example, inviable embryos in vivo, resulting from unsuccessful crosses, can be grown in vitro in a nutrient medium. This topic has been frequently discussed (see Raghavan 1977; Norstog 1979). Young embryos of cereals can be cultured in vitro to obtain regenerative cultured tissues from which many roots and shoots can be initiated. Regenerative cultures have been obtained by utilizing immature embryos in maize (Green and Phillips 1975), oats (Cummings et al. 1976), sorghum (Gamborg et al. 1977; Thomas et al. 1977), barley (Dale and Deambrogio 1979), triticale (Sharma et al. 1980), and wheat (Shimada 1978; Gosch-Wackerle et al. 1979).


Somatic Embryogenesis Immature Embryo Embryo Culture Green Spot Shoot Primordia 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • T. Shimada
    • 1
  1. 1.Research Institute of Agricultural ResourcesIshikawa Agricultural CollegeIshikawaJapan 921

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