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Trees IV pp 359-373 | Cite as

Cephalotaxus harringtonia (Japanese Plumyew)

  • Enaksha R. M. Wickremesinhe
  • R. N. Arteca
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 35)

Abstract

The plumyew (Cephalotaxus Siebold and Zuccarini ex Endl. Family Cephalotaxaceae) was first reported in 1842 (Welch 1991). The native habitat for Cephalotaxus species is Southeast Asia; however, fossils have been found in North America and Europe (Rushforth 1987). Cephalotaxus are typically dioecious; however, there have been reports of monoecious plants (Ouden and Boom 1978). Branches are opposite or in whorls, branchlets are opposite, winter buds are ovoid and blunt with numerous imbricate persistent bud scales. The leaves are spirally arranged on ascending branches, spreading in two planes on side branches, pointed at the apex with a prominent midrib, and are dark green above with two broad glaucous bands below. Flowers are axillary, male flowers have globose heads in the axil of leaves, while female flowers are in the axil of scales at the base of branchlets. Seeds are ellipsoid, drupaceous, about 5 cm long and are initially green but become purplish as they mature. The seed coat is fleshy on the outside and hard inside (Ouden and Boom 1978). The foliage resembles the yew, needles are similar in shape and arranged in two rows; however, Cephalotaxus leaves are much longer and the fruiting habit is very different, clearly distinguishing Cephalotaxaceae from Taxaceae. Cephalotaxus differs from Torreya by having leaves which are soft and blunt rather than prickly pointed (van Gelderen 1986).

Keywords

Cell Suspension Culture Root Culture Callus Culture Woody Plant Medium Coconut Milk 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Delfel NE (1977) Antitumor alkaloid metabolism and distribution in Cephalotaxus harringtonia, Japanese plumyew: whole plant and tissue culture studies. Plant Physiol 59: 62 (Abstr)Google Scholar
  2. Delfel NE (1980) The effect of nutritional factors on alkaloid metabolism in Cephalotaxus harringtonia tissue cultures. Planta Med 39: 168–179CrossRefGoogle Scholar
  3. Delfel NE, Rothfus JA (1977) Antitumor alkaloids in callus cultures of Cephalotaxus harringtonia. Phytochemistry 16: 1595–1598CrossRefGoogle Scholar
  4. Delfel NE, Rothfus JA (1979) Production of homodeoxyharringtonine and other Cephalotaxus esters by tissue culture. US patent 4, 152, 214Google Scholar
  5. Delfel NE, Smith LJ (1980) The importance of culture conditions and medium composition on the growth of Cephalotaxus harringtonia tissue cultures. Planta Med 40: 237–244CrossRefGoogle Scholar
  6. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  7. Janick J, Whipkey A (1987) Induction of shoot proliferation in Cephalotaxus harringtonia. HortScience 22: 161 (Abstr)Google Scholar
  8. Janick J, Whipkey A, Kitto SL, Frett J (1994) Micropropagation of Cephalotaxus harringtonia. HortScience 29: 120–122Google Scholar
  9. Lloyd G, McCown B (1981) Commercially feasible micropropagation of Mountain Laurel, Kalmia latifolia, by use of shoot tip culture. Int Plant Prop Soc Proc 30: 421–427Google Scholar
  10. Misawa MH, Hayashi M, Takayama S (1983) Production of antineoplastic agents by plant tissue cultures. Planta Med 49: 115–119CrossRefGoogle Scholar
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  12. Ouden PD, Boom BK (1978) Manual of cultivated conifers. Martinus Nijhoff, The HagueCrossRefGoogle Scholar
  13. Powell RG, Weisleder D, Smith CR Jr (1972) Antitumor alkaloids from Cephalotaxus harringtonia: structure and activity. J Pharm Sci 61: 1227–1230PubMedCrossRefGoogle Scholar
  14. Rushforth K (1987) Conifers. Helm, LondonGoogle Scholar
  15. Tan CTC, Luks E, Bacha DM, Steinherz P, Steinherz L, Mondora A (1987) Phase I trial of homoharringtonine in children with refractory leukemia. Cancer Treat Rep 71: 1245–1248PubMedGoogle Scholar
  16. Van Gelderen DM (1986) Conifers. Timber Press, PortlandGoogle Scholar
  17. Welch HJ (1991) The conifer manual, vol. 34. Forestry sciences. Kluwer, DordrechtCrossRefGoogle Scholar
  18. Westgate P, Emery A, Hasegawa M, Heinstein P (1987) Growth of semicontinuous cultures of Cephalotaxus harringtonia with and without coconut water. In Vitro Cell Dev Biol 23: 47A (Abstr)CrossRefGoogle Scholar
  19. Westgate PJ, Emery AH, Hasegawa PM, Heinstein PF (1991) Growth of Cephalotaxus harringtonia plant cell cultures. Appl Microbiol Biotechnol 34: 798–803CrossRefGoogle Scholar
  20. Wickremesinhe ERM, Arteca RN (1991) Organogenesis from Cephalotaxus harringtonia callus: establishment of shoots and root cultures. HortScience 26: 479 (Abstr)Google Scholar
  21. Wickremesinhe ERM, Arteca RN (1993a) Establishment of fast-growing callus and root cultures of Cephalotaxus harringtonia. Plant Cell Rep 12: 80–83CrossRefGoogle Scholar
  22. Wickremesinhe ERM, Arteca RN (1993b) Taxus callus cultures: initiation, growth optimization, characterization and taxol production. Plant Cell Tissue Organ Cult 35: 181–193CrossRefGoogle Scholar
  23. Wickremesinhe ERM, Arteca RN (1996) HPLC separation of cephalotaxine, harringtonine and homoharringtonine from callus and root cultures of Cephalotaxus harringtonia. J Liquid Chromatogr (in press)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Enaksha R. M. Wickremesinhe
    • 1
    • 2
  • R. N. Arteca
    • 1
  1. 1.Department of HorticultureThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Centre Analytical Labs. Inc.State CollegeUSA

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