Transgenic Artemisia (Wormwood)

  • S. Nin
  • A. Bennici
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 48)


Artemisia absinthium L. (wormwood), family Asteraceae, is a perennial under-shrub found in Central Europe, North America, and Asia. The plant presents the following characteristics: to 1 m high, stalks upright, abundant foliage; leaves silky pubescent on both sides, lowest petiolate, tripennate; heads in strong branched panicles, short-stalked, nodding; outer perianth oblong-linear, inner ovate with a broad membranous margin; disk flowers bisexual, the marginal flowers often female, flowering July-September, fruitlets without a pappus (Krussmann 1984). Propagation is mostly by division, but the plant, allogamous, is highly fertile.


Hairy Root Hairy Root Culture Agrobacterium Rhizogenes Plant Cell Tissue Organ Cult Tropane Alkaloid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abivardi C, Benzi G (1984) Test with the extracts of 21 medicinal plants for antifeedant activity against larvae of Pieris brassicae L. Bull Soc Entomol Suisse 57: 383–392Google Scholar
  2. Anderson FJ (1977) An illustrated hystory of the herbals. Columbia University Press, New YorkGoogle Scholar
  3. Banthorpe DV, Brown GD (1989) Two unexpected coumarin derivatives from tissue cultures of Compositae species. Phytochemistry 28: 3003–3007CrossRefGoogle Scholar
  4. Benjamin BD, Sipahimalani AT, Heble MR (1990) Tissue cultures of Artemisia pallens: organogenesis, terpenoid production. Plant Cell Tissue Organ Cult 21: 159–164CrossRefGoogle Scholar
  5. Benjamin BD, Roja G, Heble MR (1993) Agrobacterium rhizogenes mediated transformation of Rauvolfia serpentina: regeneration and alkaloid synthesis. Plant Cell Tissue Organ Cult 35: 253–257Google Scholar
  6. Berthomieu P, Jouanin L (1992) Transformation of rapid cycling cabbage (Brassica oleracea var. capitata) with Agrobacterium rhizogenes. Plant Cell Rep 11: 334–338CrossRefGoogle Scholar
  7. Birot A, Bouchez D, Casse-Debart F, Durand-Tardif M, Jouanin L, Pautot V, Robaglia C, Tepfer D, Tepfer M, Tourneur J, Vilaine F (1987) Studies and uses of the Ri plasmid of Agrobacterium rhizogenes. Plant Physiol Biochem 25: 323–335Google Scholar
  8. Chemesova II, Belenovskaya LM, Stukov AN (1987) Anti-tumor activity of flavonoids from some Artemisia species. Rastit Resur 23: 100–103Google Scholar
  9. Chialva F, Liddle PAP, Doglia G (1983) Chemotaxonomy of wormwood (Artemisia absinthium L.) I. Composition of the essential oil of several chemotypes. Z Lebensm Unters Forsch 176: 363–366Google Scholar
  10. David C, Chilton MD, Tempè J (1984) Conservation of T-DNA in plants regenerated from hairy root cultures. Bio/Technology 2: 73–76CrossRefGoogle Scholar
  11. Deans SG, Svoboda KP, Kennedy AI (1989) Biological activity of plant volatile oils and their constituents. Planta Med 55: 588CrossRefGoogle Scholar
  12. Failla MC, Maimone F, De Paolis A, Costantino P, Cardarelli M (1990) The non-conserved region of cucumopine-type Agrobacterium rhizogenes T-DNA is responsible for hairy root induction. Plant Mol Biol 15: 747–753PubMedCrossRefGoogle Scholar
  13. Flores H, Filner P (1985) In: Neumann K, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures, Springer, Berlin Heidelberg New York, pp 174–185CrossRefGoogle Scholar
  14. Gamborg OL, Moller RA, Ojina. K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50: 151–158PubMedCrossRefGoogle Scholar
  15. Hamill J, Parr A, Robins R, Rhodes M (1986) Secondary product formation by cultures of Beta vulgaris, Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep 5: 111–114CrossRefGoogle Scholar
  16. Hooykaas PJJ (1989) Transformation of plant cells via Agrobacterium. Plant Mol Biol 13: 327–336Google Scholar
  17. Inomata S, Yokoyama M, Gozu Y, Shimizu T, Yanagi M (1993) Growth pattern and ginsenoide production of Agrobacterium-transformed Panax ginseg roots. Plant Cell Rep 12: 681–686CrossRefGoogle Scholar
  18. Jaziri M, Shimomura K, Yoshimatsu K, Fauconnier M-L, Marlier M, Homès J (1995) Establishment of normal and transformed root cultures of Artemisia annua L. for artemisinin production. J Plant Physiol 145: 175–177CrossRefGoogle Scholar
  19. Jung G, Tepfer D (1987) Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production. Plant Sci 50: 145–151CrossRefGoogle Scholar
  20. Katavik V, Jelaska S, Bakran-Petricioli T, David C (1991) Host-tissue differences in transformation of pumpkin (Cucurbita pepo L.) by Agrobacterium rhizogenes. Plant Cell Tissure Organ Cult 24: 35–42CrossRefGoogle Scholar
  21. Kennedy AI, Deans SG, Svoboda KP, Gray AI, Waterman PG (1993) Volatile oil from normal and transformed root of Artemisia absinthium. Phytochemistry 32: 1449–1451CrossRefGoogle Scholar
  22. Khattak SG, Gilani SN, Ikram M (1985) Antypyretic studies on some indigenous Pakistani medicinal plants. J Ethnopharmacol 1: 45–51CrossRefGoogle Scholar
  23. Knobloch K, Pauli A, Iberl B, Weis N, Weigand H (1988) Mode of action of essential oil components on whole cells of bacteria and fungi in plate test. In: Schreier P (ed) Bioflavour 1987. De Gruyter, Berlin, pp 287–299Google Scholar
  24. Krussmann G (1984) Manual of cultivated broad-leaved trees and shrubs, vol I. BT Batsford, LondonGoogle Scholar
  25. Manders G, Otoni WC, d’Utra Vaz FB, Blackhall NW, Power JB, Davey MR (1994) Transformation of passion fruit (Passiflora edulis fv. flavicarpa Degener.) using Agrobacterium tumefaciens. Plant Cell Rep 13: 697–702CrossRefGoogle Scholar
  26. Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  27. Mano Y, Nebeshima S, Matsui C, Ohkawa H (1986) Production of tropan alkaloids by hairy root cultures of Scopolia japonica. Agric Biol Chem 50: 2715–2722CrossRefGoogle Scholar
  28. Mulder-krieger T, Verpoorte R, Barheim Svendsen A, Scheffer JJC (1988) Production of essential oils and flavours in plant cell and tissue cultures. A review. Plant Cell Tissue Organ Cult 13: 85–154CrossRefGoogle Scholar
  29. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  30. Nabeshima S, Mano Y, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Symbiosis 2: 11–18Google Scholar
  31. Nguyen C, Bourgaud F, Forlot P, Guckert A (1992) Establishment of hairy root cultures of Psoralea species. Plant Cell Rep 11: 424–427CrossRefGoogle Scholar
  32. Nin S, Schiff S, Bennici A, Magherini R (1994) In vitro propagation of Artemisia absinthium L. Adv Hort Sci 8: 145–147Google Scholar
  33. Nin S, Arfaioli P, Bosetto M (1995) Quantitative determination of some essential oil components of selected Artemisia absinthium plants. J Essent Oil Res 7: 271–277CrossRefGoogle Scholar
  34. Nin S, Morosi E, Schiff S, Bennici A (1996) Callus cultures of Artemisia absinthium L.: initiation, growth optimization and organogenesis. Plant Cell Tissue Organ Cult 45: 67–72CrossRefGoogle Scholar
  35. Nin S, Bennici A, Roselli G, Mariotti D, Schiff S, Magherini R (1997) Agrobacterium-mediated transformation of Artemisia absinthium L. (wormwood) and production of secondary metabolites. Plant Cell Rep 16: 725–730Google Scholar
  36. Oksman-Caldentey K-M, Sevôn N, Vanhala L, Hiltunen R (1994) Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus. Plant Cell Tissue Organ Cult 38: 263–272CrossRefGoogle Scholar
  37. Oliveira MM, Miguel CM, Raquel MH (1996) Transformation studies in woody fruit species. Plant Tissue Cult Biotechnol 2: 76–93Google Scholar
  38. Paniego NB, Giulietti AM (1994) Artemisia annua L.: dedifferentiated and differentiated cultures. Plant Cell Tissue Organ Cult 36: 163–168Google Scholar
  39. Pestchanker LJ, Kurina M, Giulietti AM, Giordano OS (1989) Production of dihydroleucodin from callus lines of Artemisia douglasiana Besser. Biotechnol Lett 11: 803–806CrossRefGoogle Scholar
  40. Rao VSN, Menezes AMS, Gadelha MGT (1988) Antifertility screening of some indigenous plants of Brazil. Fitoterapia 59: 17–20Google Scholar
  41. Rhodes MJC, Parr AJ, Giulietti A, Aird ELH (1994) Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures. Plant Cell Tissue Organ Cult 38: 143–151CrossRefGoogle Scholar
  42. Siemens J, Schieder 0 (1996) Transgenic plants: genetic transformation-recent developments and the state of the art. Plant Tissue Cult Biotechnol 2: 66–75Google Scholar
  43. Smith AE, Secoy DM (1981) Plants used for agricultural pest control in western Europe before 1850. Chem Indus 3: 12–17Google Scholar
  44. Spano L, Mariotti D, Pezzotti M, Damiani F, Arcioni S (1987) Hairy root transformation in alfalfa (Medicago sativa L.). Theor Appl Genet 73: 523–530CrossRefGoogle Scholar
  45. Takafumi Y, Tsutuma F (1987) Saponin production by cultures of Panax gingseng transformed with Agrobacterium rhizogenes. Plant Cell Rep 6:449–453 Google Scholar
  46. Tepfer D, Metzger L, Prost R (1989) Use of roots transformed by Agrobacterium rhizogenes in rhizosphere research: applications in studies of cadmium assimilation from sewage sludges. Plant Mol Biol 13: 295–302PubMedCrossRefGoogle Scholar
  47. Whipkey A, Simon JE, Charles DJ, Janick J (1992) In vitro production of artemisinin from Artemisia annua L. J. Herbs Spices Med Plants 1: 15–25CrossRefGoogle Scholar
  48. Willmitzer L, Sanchez-Serrano J, Buschfeld E, Schell J (1982) DNA from Agrobacterium rhizogenes is transferred to and expressed in axenic hairy root plant tissue. Mol Gen Genet 186: 16–22CrossRefGoogle Scholar
  49. Yashphe J, Feuerstein I, Barel S, Segal R (1987) The antibacterial and antispasmodic activity of Artemisia herba alba Asso. Int J Crude Drug Res 2:89–96 Google Scholar
  50. Zafar MM, Hamard ME, Hameed A (1990) Screening of Artemisia absinthium for antimalarial effects on Plasmodium berghei in mice: a preliminary report. J Ethnopharmacol 30:223–226 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • S. Nin
    • 1
  • A. Bennici
    • 1
  1. 1.Department of Plant BiologyUniversity of FlorenceFlorenceItaly

Personalised recommendations