Zusammenfassung
Mit EDTA antikoaguliertes Blut wird zunächst bei niedriger g-Zahl zentrifugiert und das überstehende plättchenreiche Plasma (PRP) von den Erythrozyten getrennt. Das PRP wird dann bei höherer g-Zahl erneut zentrifugiert, um die Thrombozyten zu sedimentieren. Wiederholtes Abnehmen des Überstandes, Resuspendieren der Thrombozyten in isotonischer Kochsalzlösung (NaCl) und erneutes Zentrifugieren, („Waschen der Thrombozyten”) bewirken eine fast vollständige Entfernung der Plasmabestandteile aus der Suspension.
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© 1988 Springer-Verlag Berlin Heidelberg
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Kiefel, V., Santoso, S. (1988). Nachweis von thrombozytären Antigenen und Antikörpern. In: Mueller-Eckhardt, C. (eds) Transfusionsmedizin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10601-3_34
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DOI: https://doi.org/10.1007/978-3-662-10601-3_34
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