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Tobacco BY-2 Cells as an Ideal Material for Biochemical Studies of Plant Cytoskeletal Proteins

  • Seiji Sonobe
  • Etsuo Yokota
  • Teruo Shimmen
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)

Abstract

Microtubules (MTs) and actin filaments (AFs) are the major cytoskeletons in plant cells. The former functions in cellular morphogenesis and cell division, while the latter functions in intracellular transport including cytoplasmic streaming and positioning of organelles. To accomplish such functions, cytoskeletons are organized into a variety of ordered structures, and to organize such structures, a variety of regulatory proteins are thought to be involved. Therefore, identification and characterization of all regulatory proteins are necessary to understand the mechanism of cellular functions performed by cytoskeletons. For this purpose, tobacco BY-2 cells have significantly contributed to physiological and biochemical approaches (Nagata et al. 1992; Shibaoka et al. 1995; Sonobe 1996). Here, we will introduce plant cytoskeletal components including microtubule-associated proteins (MAPs) and actin binding proteins (ABPs), which have been found biochemically by our group using tobacco BY-2 cells.

Keywords

Pollen Tube Cytoplasmic Streaming Preprophase Band Cytoplasmic Strand Characean Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Seiji Sonobe
    • 1
  • Etsuo Yokota
    • 1
  • Teruo Shimmen
    • 1
  1. 1.Department of Life Science, Graduate School of Science, Himeji Institute of TechnologyHarima Science Park City, HyogoJapan

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