Dynamic Behavior of Microtubules and Vacuoles at M/G1 Interface Observed in Living Tobacco BY-2 Cells

  • Fumi Kumagai
  • Arata Yoneda
  • Natsumaro Kutsuna
  • Seiichiro Hasezawa
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)


Plant cells expand mainly by water uptake into vacuoles. Although the turgor pressure of the cells is isotropic, most of the cells elongate anisotropically. This transformation of the isotropic force into the anisotropic growth is achieved by the establishment of “hoops” consisting of cellulose microfibrils (CMFs) in the cell walls. The cells can only elongate perpendicular to the newly organized CMFs, deposited at the innermost layer of the cell wall. The orientation of CMFs has been thought to be regulated by cortical microtubules (CMTs) under the cell cortex; from the observations that they run parallel to the CMFs, disturbance of CMTs resulted in aberrant cell elongation and the mutants with abnormal CMTs showed defects in elongation (for a recent review, see Baskin 2001). During the cell cycle progression, CMTs are observed only during interphase, and are then thoroughly destroyed during M phase. While CMTs are absent for about 2 h in BY-2 cells, are their any regulatory mechanisms to inhibit cell expansion into aberrant directions? And how do the daughter cells restore the next direction of expansion properly at the M/G1 interface?


Cell Cycle Progression Okadaic Acid Bright Spot Cell Plate Daughter Nucleus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Fumi Kumagai
    • 1
  • Arata Yoneda
    • 1
  • Natsumaro Kutsuna
    • 1
  • Seiichiro Hasezawa
    • 1
  1. 1.Department of Integrated Biosciences, Graduate School of Frontier SciencesThe University of TokyoChibaJapan

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