Characterisation of an Inducible/Repressible Gene Expression System in Tobacco BY-2 Cells
The identification of genes through sequencing programmes has created a great need for tools for the determination of gene function and interactions. Controllable expression of transgenes is an essential part of the armoury of available analytical approaches for the functional analysis of genes and pathways in plant biology. The ideal controllable system would function as a switch to activate and inactivate gene expression in response to a signal that has no other effect on the cells or plant. Moreover, it would have no background expression in the absence of induction, offer a high level of induced expression and be capable of rapid switch-off.
KeywordsGlucocorticoid Receptor Transgenic Tobacco Plant Gene Expression System TetR Protein Plant Transcription Factor
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