Abstract
The identification of genes through sequencing programmes has created a great need for tools for the determination of gene function and interactions. Controllable expression of transgenes is an essential part of the armoury of available analytical approaches for the functional analysis of genes and pathways in plant biology. The ideal controllable system would function as a switch to activate and inactivate gene expression in response to a signal that has no other effect on the cells or plant. Moreover, it would have no background expression in the absence of induction, offer a high level of induced expression and be capable of rapid switch-off.
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References
Ainley WM, Key JL (1990) Development of a heat shock inducible expression cassette for plants: characterization of parameters for its use in transient expression assays. Plant Mol Biol 14: 949–967
Aoyama T, Chua NH (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J 11: 605–612
Böhner S, Gatz C (2001) Characterisation of novel target promoters for the dexamethasoneinducible/tetracycline-repressible regulator TGV using luciferase and isopentenyl transferase as sensitive reporter genes. Mol Gen Genet 264: 860–870
Böhner S, Lenk I, Rieping M, Herold M, Gatz C (1999) Technical advance: transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expression. Plant J 19: 87–95
Caddick MX, Greenland AJ, Jepson I, Krause KP, Qu N, Riddell KV, Salter MG, Schuch W, Sonnewald U, Tomsett AB (1998) An ethanol inducible gene switch for plants used to manipulate carbon metabolism. Nature Biotechnol 16: 177–180
Criqui MC, Parmentier Y, Derevier A, Shen WH, Dong A, Genschik P (2000) Cell cycle-dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells. Plant J 24: 763–773
David KM, Perrot-Rechenmann C (2001) Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression. Plant Physiol 125: 1548–1553
Faiss M, Strnad M, Redig P, Doleal K, Hanu J, Van Onckelen H, Schmülling T (1996) Chemically induced expression of the rolC-encoded beta-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosidases in planta. Plant J 10: 33–46
Faiss M, Zalubilova J, Strnad M, Schmülling T (1997) Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants. Plant J 12: 401–415
Gatz C (1997) Chemical control of gene expression. Annu Rev Plant Physiol Plant Mol Biol 48:89– 108
Gatz C, Quail PH (1988) Tn10-encoded tet repressor can regulate an operator-containing plant promoter. Proc Natl Acad Sci USA 85: 1394–1397
Gatz C, Frohberg C, Wendenburg R (1992) Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants. Plant J 2: 397–404
Haseloff J, Siemering KR, Prasher DC, Hodge S (1997) Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94: 2122–2127
Kang HG, Fang Y, Singh KB (1999) A glucocorticoid-inducible transcription system causes severe growth defects in Arabidopsis and induces defense-related genes. Plant J 20: 127–133
Love J, Allen GC, Gatz C, Thompson WF (2002) Differential Top10 promoter regulation by six tetracycline analogues in plant cells. J Exp Bot 53: 1871–1877
Martinez A, Sparks C, Hart CA, Thompson J, Jepson I (1999) Ecdysone agonist inducible transcription in transgenic tobacco plants. Plant J 19: 97–106
Masgrau C, Altabella T, Farras R, Flores D, Thompson AJ, Besford RT, Tiburcio AF (1997) Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. Plant J 11: 465–473
Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the “Helâ cell in the cell biology of higher plants. Int Rev Cytol 132: 1–30
Nishihama R, Soyano T, Ishikawa M, Araki S, Tanaka H, Asada T, Irie K, Ito M, Terada M, Banno H, Yamazaki Y, Machida Y (2002) Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. Cell 109: 87–99
Rieping M, Fritz M, Prat S, Gatz C (1994) A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants. Plant Cell 6: 1087–1098
Röder FT, Schmülling T, Gatz C (1994) Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants. Mol Gen Genet 243: 32–38
Severin K, Schöffl F (1990) Heat-inducible hygromycin resistance in transgenic tobacco. Plant Mol Biol 15: 827–833
Weinmann P, Gossen M, Hillen W, Bujard H, Gatz C (1994) A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J 5: 559–569
Zuo J, Chua NH (2000) Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotechnol 11: 146–151
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Planchais, S., Kiuna, G., Armstrong, G., Murray, J.A.H. (2004). Characterisation of an Inducible/Repressible Gene Expression System in Tobacco BY-2 Cells. In: Nagata, T., Hasezawa, S., Inzé, D. (eds) Tobacco BY-2 Cells. Biotechnology in Agriculture and Forestry, vol 53. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10572-6_20
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DOI: https://doi.org/10.1007/978-3-662-10572-6_20
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-07305-2
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