Characterisation of an Inducible/Repressible Gene Expression System in Tobacco BY-2 Cells

  • Séverine Planchais
  • Gachao Kiuna
  • Graham Armstrong
  • James A. H. Murray
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)

Abstract

The identification of genes through sequencing programmes has created a great need for tools for the determination of gene function and interactions. Controllable expression of transgenes is an essential part of the armoury of available analytical approaches for the functional analysis of genes and pathways in plant biology. The ideal controllable system would function as a switch to activate and inactivate gene expression in response to a signal that has no other effect on the cells or plant. Moreover, it would have no background expression in the absence of induction, offer a high level of induced expression and be capable of rapid switch-off.

Keywords

Toxicity Depression Agar Arginine Dexamethasone 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ainley WM, Key JL (1990) Development of a heat shock inducible expression cassette for plants: characterization of parameters for its use in transient expression assays. Plant Mol Biol 14: 949–967PubMedCrossRefGoogle Scholar
  2. Aoyama T, Chua NH (1997) A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J 11: 605–612PubMedCrossRefGoogle Scholar
  3. Böhner S, Gatz C (2001) Characterisation of novel target promoters for the dexamethasoneinducible/tetracycline-repressible regulator TGV using luciferase and isopentenyl transferase as sensitive reporter genes. Mol Gen Genet 264: 860–870PubMedCrossRefGoogle Scholar
  4. Böhner S, Lenk I, Rieping M, Herold M, Gatz C (1999) Technical advance: transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expression. Plant J 19: 87–95PubMedCrossRefGoogle Scholar
  5. Caddick MX, Greenland AJ, Jepson I, Krause KP, Qu N, Riddell KV, Salter MG, Schuch W, Sonnewald U, Tomsett AB (1998) An ethanol inducible gene switch for plants used to manipulate carbon metabolism. Nature Biotechnol 16: 177–180CrossRefGoogle Scholar
  6. Criqui MC, Parmentier Y, Derevier A, Shen WH, Dong A, Genschik P (2000) Cell cycle-dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells. Plant J 24: 763–773PubMedCrossRefGoogle Scholar
  7. David KM, Perrot-Rechenmann C (2001) Characterization of a tobacco Bright Yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression. Plant Physiol 125: 1548–1553PubMedCrossRefGoogle Scholar
  8. Faiss M, Strnad M, Redig P, Doleal K, Hanu J, Van Onckelen H, Schmülling T (1996) Chemically induced expression of the rolC-encoded beta-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosidases in planta. Plant J 10: 33–46CrossRefGoogle Scholar
  9. Faiss M, Zalubilova J, Strnad M, Schmülling T (1997) Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants. Plant J 12: 401–415PubMedCrossRefGoogle Scholar
  10. Gatz C (1997) Chemical control of gene expression. Annu Rev Plant Physiol Plant Mol Biol 48:89– 108Google Scholar
  11. Gatz C, Quail PH (1988) Tn10-encoded tet repressor can regulate an operator-containing plant promoter. Proc Natl Acad Sci USA 85: 1394–1397PubMedCrossRefGoogle Scholar
  12. Gatz C, Frohberg C, Wendenburg R (1992) Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants. Plant J 2: 397–404PubMedGoogle Scholar
  13. Haseloff J, Siemering KR, Prasher DC, Hodge S (1997) Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94: 2122–2127PubMedCrossRefGoogle Scholar
  14. Kang HG, Fang Y, Singh KB (1999) A glucocorticoid-inducible transcription system causes severe growth defects in Arabidopsis and induces defense-related genes. Plant J 20: 127–133PubMedCrossRefGoogle Scholar
  15. Love J, Allen GC, Gatz C, Thompson WF (2002) Differential Top10 promoter regulation by six tetracycline analogues in plant cells. J Exp Bot 53: 1871–1877PubMedCrossRefGoogle Scholar
  16. Martinez A, Sparks C, Hart CA, Thompson J, Jepson I (1999) Ecdysone agonist inducible transcription in transgenic tobacco plants. Plant J 19: 97–106PubMedCrossRefGoogle Scholar
  17. Masgrau C, Altabella T, Farras R, Flores D, Thompson AJ, Besford RT, Tiburcio AF (1997) Inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. Plant J 11: 465–473PubMedCrossRefGoogle Scholar
  18. Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the “Helâ cell in the cell biology of higher plants. Int Rev Cytol 132: 1–30CrossRefGoogle Scholar
  19. Nishihama R, Soyano T, Ishikawa M, Araki S, Tanaka H, Asada T, Irie K, Ito M, Terada M, Banno H, Yamazaki Y, Machida Y (2002) Expansion of the cell plate in plant cytokinesis requires a kinesin-like protein/MAPKKK complex. Cell 109: 87–99PubMedCrossRefGoogle Scholar
  20. Rieping M, Fritz M, Prat S, Gatz C (1994) A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants. Plant Cell 6: 1087–1098PubMedGoogle Scholar
  21. Röder FT, Schmülling T, Gatz C (1994) Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants. Mol Gen Genet 243: 32–38PubMedCrossRefGoogle Scholar
  22. Severin K, Schöffl F (1990) Heat-inducible hygromycin resistance in transgenic tobacco. Plant Mol Biol 15: 827–833PubMedCrossRefGoogle Scholar
  23. Weinmann P, Gossen M, Hillen W, Bujard H, Gatz C (1994) A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J 5: 559–569PubMedCrossRefGoogle Scholar
  24. Zuo J, Chua NH (2000) Chemical-inducible systems for regulated expression of plant genes. Curr Opin Biotechnol 11: 146–151PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Séverine Planchais
    • 1
  • Gachao Kiuna
    • 1
  • Graham Armstrong
    • 1
  • James A. H. Murray
    • 1
  1. 1.Institute of BiotechnologyUniversity of CambridgeCambridgeUK

Personalised recommendations