Improvements of the Molecular Toolbox for Cell Cycle Studies in Tobacco BY-2 Cells
The cell cycle is a highly ordered process consisting of four consecutive phases, G1, S (DNA replication), G2, and M (mitosis) that lead to the formation of two daughter cells. During G1 phase, the cell monitors its own environment and size, before entering the replication phase. Once committed, the nuclear DNA is duplicated and the cell goes into a state of apparent inactivity, called G2 phase. The G2 phase can be regarded as a safety gap whereby the cell ensures that DNA replication has been completed before mitosis starts. By cytological analysis various stages are distinguished within the M phase, including nuclear division or karyokinesis and cytoplasmic division, also referred to as cytokinesis. Traditionally, mitosis is divided into subsequent stages designated prophase, prometaphase, metaphase, anaphase, and telophase. Additionally, a sixth stage can be discerned that takes place at the end of mitosis, during which cytokinesis occurs.
KeywordsCortical Microtubule loxP Site Destination Vector Preprophase Band Cell Cycle Study
Unable to display preview. Download preview PDF.
- Bachem CWB, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RGF (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745– 753Google Scholar
- Durrant WE, Rowland O, Piedras P, Hammond-Kosack KE, Jones JDG (2000) cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. Plant Cell 12: 963–977Google Scholar
- Persson S, Love J, Tsou P-L, Robertson D, Thompson WF, Boss WF (2002) When a day makes a difference. Interpreting data from endoplasmic reticulum-targeted green fluorescent protein fusions in cells grown in suspension culture. Plant Physiol 128: 341–344Google Scholar
- Qin L, Overmars H, Helder J, Popeijus H, Rouppe van der Voort J, Groenink W, van Koert P, Schots A, Bakker J, Smant G (2000) An efficient cDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato cyst nematode Globodera rostochiensis. Mol Plant-Microbe Interact 13: 830–836PubMedCrossRefGoogle Scholar
- Renaudin J-P, Doonan JH, Freeman D, Hashimoto J, Hirt H, Inzé D, Jacobs T, Kouchi H, Rouzé P, Sauter M, Savouré A, Sorrell DA, Sundaresan V, Murray JAH (1996) Plant cyclins: a unified nomenclature for plant A-, B- and D-type cyclins based on sequence organization. Plant Mol Biol 32: 1003–1018Google Scholar
- Scott A, Wyatt S, Tsou P-L, Robertson D, Allen NS (1999) Model system for plant cell biology: GFP imaging in living onion epidermal cells. Biotechniques 26: 1125–1132Google Scholar
- Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2000) Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep 1:287– 292Google Scholar
- van der Biezen EA, Juwana H, Parker JE, Jones JDG (2000) cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana. Mol Plant-Microbe Interact 13: 895–898Google Scholar
- van der Meijden CMJ, Lapointe DS, Luong MX, Peric-Hupkes D, Cho B, Stein JL, van Wijnen AJ, Stein GS (2002) Gene profiling of cell cycle progression through S-phase reveals sequential expression of genes required for DNA replication and nucleosome assembly. Cancer Res 62: 3233–3243PubMedGoogle Scholar
- Weld R, Heinemann J, Eady C (2001) Transient GFP expression in Nicotiana plumbaginifolia suspension cells: the role of gene silencing, cell death and T-DNA loss. Plant Mol Biol 45:377– 385Google Scholar