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In Vitro Transcription Systems from BY-2 Cells

  • Chapter
Tobacco BY-2 Cells

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 53))

Abstract

The in vitro transcription system is a powerful method to dissect molecular mechanisms of transcription of genes in question, usually performed in cellular or nuclear extracts containing transcription apparatuses with exogenous DNA templates and four nucleoside triphosphate (NTP) substrates. The first eukaryotic system was reported by Weil et al. (1979). They carried out in vitro transcription using a cytosolic extract and purified Pol II from HeLa cells. Manley et al. (1980) followed with in vitro assays using a whole cell extract from HeLa cells. In 1983, Dignam et al. (1983) established a standard procedure to prepare nuclear extracts from HeLa cells. Based on these efforts, many other in vitro systems have been established from different eukaryotic species, for example, yeast, mouse, and fruit fly. Since then, many significant discoveries have been made with these in vitro transcription systems.

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Authors and Affiliations

  1. Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, 467-8501, Nagoya, Japan

    Yasushi Yukawa & Masahiro Sugiura

Authors
  1. Yasushi Yukawa
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  2. Masahiro Sugiura
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Editors and Affiliations

  1. Graduate School of Science, Department of Biological Sciences, University of Tokyo, 7-3-1 Hongo, 113-0033, Bunkyo-ku, Tokyo, Japan

    Toshiyuki Nagata

  2. Graduate School of Science, Department of Integral Biosciences, University of Tokyo, 5-1-5 Kashiwanoha, 227-8562, Kashiwa-shi, Chiba, Japan

    Seiichiro Hasezawa

  3. Department of Plant Systems Biology, VIB/Ghent University, Technologiepark 927, 9052, Ghent, Belgium

    Dirk Inzé

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Yukawa, Y., Sugiura, M. (2004). In Vitro Transcription Systems from BY-2 Cells. In: Nagata, T., Hasezawa, S., Inzé, D. (eds) Tobacco BY-2 Cells. Biotechnology in Agriculture and Forestry, vol 53. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10572-6_18

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  • DOI: https://doi.org/10.1007/978-3-662-10572-6_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-07305-2

  • Online ISBN: 978-3-662-10572-6

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