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Cell Wall Dynamics in Tobacco BY-2 Cells

  • Ryusuke Yokoyama
  • Daisuke Tanaka
  • Takeshi Fujino
  • Takao Itoh
  • Kazuhiko Nishitani
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 53)

Abstract

The plant cell wall is composed of several different classes of macromolecules, including polysaccharides, structural proteins and aromatic substances. These components are integrated into a kind of supermolecule by means of weak inter- and intra-molecular interactions, as well as by cross-linking with covalent bonds. Within this architecture are a wide variety of cell wall enzymes, most of which are involved in the construction, maintenance and restructuring of its own architecture. As a result of the actions of these enzymes, the cell wall undergoes drastic changes in its molecular architecture in such a way that allows controlled cell wall expansion and deformation, thereby playing crucial roles in plant growth and morphogenesis. In addition to the morphological roles, the plant cell wall plays a wide range of physiological functions, which include the defense system against pathogens, translocation of nutrients and transduction of chemical signals within plants.

Keywords

Plant Cell Wall Cell Wall Protein Cortical Microtubule Cell Wall Regeneration Xyloglucan Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79: 568–570PubMedCrossRefGoogle Scholar
  2. Campbell P, Braam J (1999) Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions. Trends Plant Sci 4: 361–382PubMedCrossRefGoogle Scholar
  3. de Marco A, Roubelakis-Angelakis KA (1996a) The complexity of enzymic control of hydrogen peroxide may affect the regeneration potential of plant protoplasts. Plant Physiol 110: 137–145PubMedGoogle Scholar
  4. de Marco A, Roubelakis-Angelakis KA (1996b) Hydrogen peroxide plays a bivalent role in the regeneration of protoplasts. J Plant Physiol 149: 109–114CrossRefGoogle Scholar
  5. de Marco A, Guzzardi P, Jamet E (1999) Isolation of tobacco isoperoxidases accumulated in cell-suspension culture medium and characterization of activities related to cell wall metabolism. Plant Physiol 120: 371–381PubMedCrossRefGoogle Scholar
  6. Fisher DD, Cyr RJ (1998) Extending the microtubule/microfibril paradigm. Cellulose synthesis is required for normal cortical microtubule alignment in elongating cells. Plant Physiol 116: 1043–1051Google Scholar
  7. Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ (1992) Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem J 282:821– 828Google Scholar
  8. Giddings TH Jr, Staehelin LA (1988) Spatial relationship between microtubules and plasma-membrane rosettes during the deposition of primary wall microfibrils in Costerium sp. Planta 173: 22–30CrossRefGoogle Scholar
  9. Granger CL, Cyr RJ (2000) Microtubule reorganization in tobacco BY-2 cells stably expressing GFP-MBD. Planta 210: 502–509PubMedCrossRefGoogle Scholar
  10. Granger CL, Cyr RJ (2001) Use of abnormal preprophase bands to decipher division plane determination. J Cell Science 114: 599–607PubMedGoogle Scholar
  11. Hasezawa S, Syono K (1983) Hormonal control of elongation of tobacco cells derived from protoplasts. Plant Cell Physiol 24: 127–132Google Scholar
  12. Hasezawa S, Nozaki H (1999) Role of cortical microtubules in the orientation of cellulose microfibril deposition in higher-plant cells. Protoplasma 209: 98–104PubMedCrossRefGoogle Scholar
  13. Hasezawa S, Hogetsu T, Syono K (1988) Rearrangement of cortical microtubules in elongating cells derived from tobacco protoplasts: a time-course observation by immunofluorescence microscopy. J Plant Physiol 133: 46–51CrossRefGoogle Scholar
  14. Hogetsu T, Shibaoka H, Shimokoriyama M (1974). Involvement of cellulose synthesis in actions of gibberellin and kinetin on cell expansion. 2,6-dichlorobenzonitrile as a new cellulose-synthesis inhibitor. Plant Cell Physiol 15: 389–393Google Scholar
  15. Hong Z, Delauney AJ, Verma DPS (200 1) A cell plate-specific callose synthase and its interaction with phragmoplastin. Plant Cell 13: 755–768Google Scholar
  16. Ishikawa H, Evans ML (1995) Specialized zones of development in root. Plant Physiol 109: 725–727PubMedGoogle Scholar
  17. Ito H, Nishitani K (1999) Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer. Plant Cell Physiol 40: 1172–1176CrossRefGoogle Scholar
  18. Jacobs T (1997) Why do plant cells divide? Plant Cell 9: 1021–1029Google Scholar
  19. Kakimoto T, Shibaoka H (1988) Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells. Protoplasma Suppl 2: 95–103CrossRefGoogle Scholar
  20. Kakimoto T, Shibaoka H (1992) Synthesis of polysaccharides in phragmoplasts isolated from tobacco BY-2 cells. Plant Cell Physiol 33: 353–361Google Scholar
  21. Kobayashi M, Ohno K, Matoh T (1997) Boron nutrition of cultured tobacco BY-2 cells. II.Google Scholar
  22. Characterization of the boron-polysaccharide complex. Plant Cell Physiol 38:676–683Google Scholar
  23. Kost B, Spielhofer P, Chua NH. (1998) A GFP-mouse talin fusion protein labels plant actinGoogle Scholar
  24. filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes. Plant J 16:393–401Google Scholar
  25. Kumagai F, Yoneda A, Tomida T, Sano T, Nagata T, Hasezawa S (200 1) Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells. Plant Cell Physiol 42: 723–732Google Scholar
  26. Link BM, Cosgrove DJ (1998) Acid-growth response and alpha-expansins in suspension cultures of Bright Yellow 2 tobacco. Plant Physiol 118: 907–916PubMedCrossRefGoogle Scholar
  27. Marc J, Granger CL, Brincat J, Fisher DD, Kao T, McCubbin AG, Cyr RJ (1998) A GFP-MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells. Plant Cell 10: 1927–1940PubMedGoogle Scholar
  28. Mathur J, Koncz C (1998) In: Martínez-Zapater JM, Salinas J (eds) Arabidopsis protocols, methods in molecular biology, vol 82, Human Press, Totowa, NJ, pp 267–276Google Scholar
  29. Matoh T, Ishigaki K, Mizutani M, Matsunaga W, Takabe K (1992) Boron nutrition of cultured tobacco BY-2 cells. I. Requirement for and intracellular localization of boron and selection of cells tolerate low levels of boron. Plant Cell Physiol 33: 1135–1141Google Scholar
  30. Matoh T, Takasaki M, Kobayashi M, Takabe K (2000) Boron nutrition of cultured tobacco BY-2 cells. III. Characterization of the boron-rhamnogalacturonan II complex in cells acclimated to low levels of boron. Plant Cell Physiol 41: 363–366Google Scholar
  31. Matsuoka K, Nakamura K (1991) Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. Proc Natl Acad Sci USA 88: 834–838PubMedCrossRefGoogle Scholar
  32. Moore PJ, Staehelin LA (1988) Immunogold localization of the cell wall-matrix polysaccharides rhamnogalacturonan I and xyloglucan during cell expansion and cytokinesis in Trifolium pratense L.; implication for secretory pathways. Planta 178: 353–366Google Scholar
  33. Nagata T, Okada K, Kawada T, Takebe I (1981) Delivery of tobacco mosaic virus RNA into plant protoplast mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet 184: 161–165Google Scholar
  34. Nagata T, Okada K, Takebe I (1982) Mitotic protoplasts and their infection with tobacco mosaic virus RNA encapsulated in liposomes. Plant Cell Rep 1: 250–252CrossRefGoogle Scholar
  35. Nagata T, Okada K, Kawada T, Takebe I (1987) Cauliflower mosaic virus 35S promoter directs S phase specific expression in plant cells. Mol Gen Genet 207: 242–244CrossRefGoogle Scholar
  36. Nagata T, Nemoto Y, Hasezawa S (1992) Tobacco BY-2 cell line as the “Hela” cell in the cell biology of higher plants. Int Rev Cytol 132: 1–30CrossRefGoogle Scholar
  37. Nakagawa N, Sakurai N (1998) Increase in the amount of celA1 protein in tobacco BY-2 cells by a cellulose biosynthesis inhibitor, 2,4-dichlorobenzonitrile. Plant Cell Physiol 39: 779–785PubMedCrossRefGoogle Scholar
  38. Nebenfuhr A, Gallagher LA, Dunahay TG, Frohlick JA, Mazurkiewicz AM, Meehl JB, Staehelin, LA (1999). Stop-and-go movements of plant Golgi stacks are mediated by the acto-myosin system. Plant Physiol 121: 1127–1141PubMedCrossRefGoogle Scholar
  39. Nebenfuehr A, Frohlick JA, Staehelin LA (2000) Redistribution of Golgi stacks and other organelles during mitosis and cytokinesis in plant cells. Plant Physiol 124: 135–151CrossRefGoogle Scholar
  40. Nishitani K (1997) The role of endoxyloglucan transferase in the organization of plant cell walls. Int Rev Cytol 173: 157–206PubMedCrossRefGoogle Scholar
  41. Nishitani K (1998) Construction and restructuring of the cellulose-xyloglucan framework in the apoplast as mediated by the xyloglucan-related family–a hypothetical scheme. J Plant Res 111: 159–166CrossRefGoogle Scholar
  42. Nishitani K (2002) Genome-based approach to study the mechanism by which cell-wall type is defined and constructed by means of collaborative actions of wall-related enzymes. J Plant Res 115: 303–307PubMedCrossRefGoogle Scholar
  43. Nishitani K, Tominaga R (1992) Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J Biol Chem 267: 21058–21064PubMedGoogle Scholar
  44. Rempel HC, Nelson LM (1995) Analysis of conditions for Agrobacterium-mediated transformation of tobacco cells in suspension. Transgenic Res 4: 199–207CrossRefGoogle Scholar
  45. Sabba RP, Durso NA, Vaughn C (1999) Structural and immunocytochemical characterization of the walls of dichlobenil-habituated BY-2 tobacco cells. Int J Plant Sci 160: 275–290CrossRefGoogle Scholar
  46. Shedletzky E, Shmuel M, Delmer DP, Lamport DTA (1990) Adaptation and growth of tomato cells on the herbicide 2,6-dichlorobenzonitrile leads to production of unique cell walls virtually lacking a cellulose-xyloglucan network. Plant Physiol 94: 980–987PubMedCrossRefGoogle Scholar
  47. Shedletzky E, Shmuel M, Trainin T, Kalman S, Delmer D (1992) Cell wall structure in cells adapted to growth on the cellulose-synthesis inhibitor 2,6-dichlorobenzonitrile. Plant Physiol 100: 120–130PubMedCrossRefGoogle Scholar
  48. Sun J, Kawazu T, Kimura T, Karita S, Sakka K, Ohmiya K (1997) High expression of the xylanase B gene from Clostridium sterorarium in tobacco cells. J Ferment Bioeng 84: 219–223CrossRefGoogle Scholar
  49. Warington K (1923) The effect of boric acid and borax on the broad bean and certain other plants. Ann Bot 37: 629–672Google Scholar
  50. Williamson RE (1991) Orientation of cortical microtubules in interphase plant cells. Int Rev Cytol 129: 135–206CrossRefGoogle Scholar
  51. Yokoyama R, Nishitani K (2001) Endoxyloglucan transferase is localized both in the cell plate and in the secretory pathway destined for the apoplast in tobacco cells. Plant Cell Physiol 42: 292–300PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2004

Authors and Affiliations

  • Ryusuke Yokoyama
    • 1
  • Daisuke Tanaka
    • 1
  • Takeshi Fujino
    • 2
  • Takao Itoh
    • 2
  • Kazuhiko Nishitani
    • 1
  1. 1.Biological Institute, Graduate School of SciencesTohoku UniversitySendaiJapan
  2. 2.Wood Research InstituteKyoto UniversityJapan

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